Effect of colchicine on microtubules in the melanophores of Fundulus heteroclitus

Wikswo, Muriel A.; Novales, Ronald R.

doi:10.1016/s0022-5320(72)90063-9

Cited by 53 publications

(18 citation statements)

References 39 publications

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“…Even though observations of cytoskeletal participation in pigment translocation of different chromatophores are not always consistent, it is generally accepted that the cytoskeleton is somehow involved. All three of the known cytoskeletal components, the microtubules, actin filaments and intermediate filaments, have been implicated in pigment translocation in chromatophores; the directness and degree of their involvement appear to vary, depending on the type of chromatophore under investigation (erythrophore, melanophore, or xanthophore) and the Several observations support the notion that pigment granules use microtubules as intracellular tracks [Wikswo and Novales, 1972;Murphy and Tilney, 1974;McNiven et al, 1984], and that microtubule-dependent motors provide the motive force in pigment movement [Beckerle and Porter, 1982;Clark and Rosenbaum, 1982;Rodionov et al, 1991]. The involvement of other cytoskeletal elements is more controversial.…”

Section: Introductionmentioning

confidence: 87%

Characterization of a novel filament system in goldfish xanthophores

Wang

Chen

Fong

et al. 1997

Cell Motil. Cytoskeleton

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Section: Introductionmentioning

confidence: 87%

Characterization of a novel filament system in goldfish xanthophores

Wang

Chen

Fong

et al. 1997

Cell Motil. Cytoskeleton

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“…The main function of these large cells, which have a well-developed radial system of microtubules (13), is a fast and synchronous redistribution of hundreds of pigment granules that is completely dependent on integrity of cytoplasmic microtubules (14,15) and ATP as an energy source (16)(17)(18)(19). As in many other eukaryotic cells, the plus ends of microtubules in melanophores are at the cell periphery, and the minus ends are associated with the centrosome (20).…”

mentioning

confidence: 99%

Kinesin is responsible for centrifugal movement of pigment granules in melanophores.

Rodionov

Gyoeva

Gelfand

1991

Proc. Natl. Acad. Sci. U.S.A.

164

111

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Kinesin is a mechanochemical ATPase that induces translocation of latex beads along microtubules and microtubule gliding on a glass surface. This protein is thought to be a motor for the movement of membranous organelles in cells. Recently Hollenbeck and Swanson [Hollenbeck, P. J. & Swanson, J. A. (1990) Nature (London) 346, [864][865][866] showed that kinesin is involved in the positioning of tubular Iysosomes in macrophages. However, the role of this protein in the movement of organelles was not yet clear. We used a polyclonal antibody against the kinesin heavy chain that inhibited kinesindependent microtubule gliding in vitro to study the role of kinesin in the movement of pigment granules in melanophores of the teleost black tetra (Gymnocorymbus ternetzi). Microinjection of the antibody into cultured melanophores did not produce any specific effect on the aggregation o pigment granules in melanophores, but it did result in a strong dosedependent inhibition of the dispersion. Immunoblotting of melanophore extracts showed that the kinesin antibody reacted in these cells with a single protein component with a molecular mass of 135 kDa. Thus, kinesin is responsible for the movement of pigment granules from the center to the periphery of the melanophore.

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“…This situation is true of neuron axons (e.g. 24,25), of the heliozoan axopodia (22,26), and of the suctorian tentacles (4,23) and, to a lesser extent, it is true in the processes of melanophores (5,12,19,28,29) and chromatophores (21). It could be argued that the proximal association between the microtubules and particles in these cells is more a result of the small diameter of the cell process than of an actual physical linkage between the two.…”

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confidence: 99%

Evidence for firm linkages between microtubules and membrane-bounded vesicles.

Allen¹

1975

The Journal of Cell Biology

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Direct evidence is presented in support of the widely held idea that membrane-bounded vesicles can bind firmly to microtubules. This is shown in P. caudatum which contains ribbons of straight microtubules located in open cytoplasm and uniquely associated with the disk-shaped vesicles. These vesicles frequently lie flat against the face of the ribbons at a constant distance of 30-40 nm. Under certain conditions the ribbons are compressed into zigzag pattern, but the vesicles continue to maintain their 30-40 nm spacing with the tubules and The author's interpretation of this phenomena is that the vesicles and the microtubules are strongly bound together. This interaction appears to be via a filamentous material rather than bridges.

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Effect of colchicine on microtubules in the melanophores of Fundulus heteroclitus

Cited by 53 publications

References 39 publications

Characterization of a novel filament system in goldfish xanthophores

Characterization of a novel filament system in goldfish xanthophores

Kinesin is responsible for centrifugal movement of pigment granules in melanophores.

Evidence for firm linkages between microtubules and membrane-bounded vesicles.

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