Effect of Cold Atmospheric Plasma (CAP) on Osteogenic Differentiation Potential of Human Osteoblasts

Abstract

Bone regeneration after oral and maxillofacial surgery is a long-term process, which involves various mechanisms. Recently, cold atmospheric plasma (CAP) has become known to accelerate wound healing and have an antimicrobial effect. Since the use of CAP in dentistry is not yet established, the aim of the present study was to investigate the effect of CAP on human calvaria osteoblasts (HCO). HCO were treated with CAP for different durations of time and distances to the cells. Cell proliferation was determined by MTT assay and cell toxicity by LDH assay. Additionally, RT-qPCR was used to investigate effects on osteogenic markers, such as alkaline phosphatase (ALP), bone morphogenic protein (BMP)2, collagen (COL)1A1, osteonectin (SPARC), osteoprotegerin (OPG), osterix (OSX), receptor activator of NF-κB (RANK), RANK Ligand (RANKL), and Runt-related transcription factor (RUNX)2. There were small differences in cell proliferation and LDH release regarding treatment duration and distance to the cells. However, an increase in the expression of RANK and RANKL was observed at longer treatment times. Additionally, CAP caused a significant increase in mRNA expression of genes relevant to osteogenesis. In conclusion, CAP has a stimulating effect on osteoblasts and may thus represent a potential therapeutic approach in the regeneration of hard tissue defects.

Modulation of Inflammatory Responses by a Non-Invasive Physical Plasma Jet during Gingival Wound Healing

“…In this study, we focused on the direct application of NIPP as a simple, inexpensive and non-harmful method for improving wound healing. As we have already demonstrated a wound-healing and mineralisation-promoting effect for hard tissue in previous studies [ 31 , 75 ], NIPP could be used by dentists for different procedures. For example, the antimicrobial effect would allow an application in the field of immediate implantation.…”

Anti‐osteoporosis effects of mammalian lignans and their precursors from flaxseed and safflower seed using zebrafish model

“…Runx2 and SP7 are expressed in the early and late stages of osteoblast differentiation, respectively, and are essential for bone formation. They have been used as important biomarkers for evaluating the stimulating effects of various factors and reagents on osteoblasts (Eggers et al., 2022; Li, Zhou, et al., 2021). SDG and TCL significantly upregulated the expression of Runx2 and SP7 ( p < 0.001 vs. ALX group).…”

Opinions from five oral specialists on 3D printing in the challenge of customized oral healthcare. What can plasma technology bring?

“…[26,27] Thus, the materials used in 3D printing could be activated by plasma and plasma technology could render these biomaterials bioactive. [28,29] The modification of the gas or the parameters of the plasma induces the modification of the physicochemical species produced and therefore of the properties of the plasma. The wettability, the surface state, or even surface energy can be modified.…”

“…[ 26,27 ] Thus, the materials used in 3D printing could be activated by plasma and plasma technology could render these biomaterials bioactive. [ 28,29 ]…”