Effect of cold plasma on periodontal wound healing—an in vitro study

Abstract

Our study demonstrates the application of CAP in the treatment of oral pathologies suggesting a promising future treatment approach.

The beneficial effect of cold atmospheric plasma on parameters of molecules and cell function involved in wound healing in human osteoblast-like cells in vitro

“…Similar findings concerning an elevated gene expression after CAP treatment were also observed in gingival fibroblasts and periodontal ligament cells [4,38]. CAP treatment of MG63 cells also significantly upregulated Ki67 and PCNA, indicating a process of active cell proliferation.…”

“…The ambient air gases-mainly nitrogen, but also oxygen and argon, are converted into CAP: the CAP can be used at 5 levels of intensity, modulating the high voltage (3-18 kV). MG63 cells were exposed to CAP treatment for 60 s as previously described [4]. Optimal conditions were selected after preliminary experiments.…”

“…Various effects of CAP on gene regulation have been observed in different cell types such as keratinocytes or gingival fibroblasts [37,38]. Additionally, we have recently shown CAP effects on periodontal cells in vitro [4]. Apart from these soft tissue cells other resident oral cell types should be analysed.…”

“…The healing post-operative process after oral surgery interventions include the repair and regeneration of soft and hard tissues [ 1 – 3 ]. In own previous studies it was demonstrated that cold atmospheric plasma (CAP) could positively influence periodontal wound healing by change of critical molecules at transcriptional level, increase of cell viability and wound closure rate in human periodontal ligament cells (hPDL) [ 4 ]. The healing of hard tissue is a major step for the entire regeneration of an affected area, forming its stabilizing scaffold.…”

Oralchirurgie: Mediatoren der Wundheilung

“…B. TGF-β1 und der insulinähnliche Wachstumsfaktor-1 (Insulin-like growth factor 1, IGF-1), in humanen parodontalen Ligamentzellen (PDL) nachweisen, die sich durch positive Stimulation der In-vitro-Wundfüllrate, Zellproliferation und Adhäsion, Synthese von Wachstumsfaktoren und Kollagen sowie Mineralisierung auszeichnen [19]. Als relativ neue Anwendungsmöglichkeit ist zudem die Kaltplasmatherapie (cold atmospheric plasma, CAP) in den Fokus gerückt, die bei chronischen Wunden anwendbar ist und möglicherweise die Wundheilung positiv beeinflussen kann [20].…”