Effect of Coliphage Entrapment on its Interaction with the Host Bacterium

Fattouh, A Faiza; Aassar, A Samy El; E, Farag; Hassan, Wail

doi:10.3923/biotech.2003.94.100

Cited by 2 publications

(5 citation statements)

References 17 publications

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“…(36) In the present study, VPS1 and APS2 assigned to order caudovirales as they have long tail and double stranded DNA. Our results were in accordance with that reported by Fattouh et al, (16) and Pereira et al, (10) where the tailed phages predominated other morphotypes. VPS1, APS2 and APS3 were found to belong to family Siphoviridae, Myoviridae and Podoviridae, respectively as was proposed by Murphy et al (37) APS3 belonged to family Podoviridae.…”

Section: Parahaemolyticus V Alginolyticus Andsupporting

confidence: 94%

“…In the current work, APS3 was entrapped into calcium alginate beads and its efficiency in controlling the growth rate of A. hydrophila was studied. The entrapped phage particles were better in its effect than the free phage (significant at p<0.05) which was in agreement with that reported by Fattouh et al (16) This could be attributed to the hydrophobicity of calcium alginate beads entrapping the phage, retaining the integrity of the beads and preventing their degradation over the course of experiment and thus allowing more surface of contact between the phage and its host as was explained by Kim et al (26) In the present study, the efficiency of the entrapped phage particles in reducing the growth rate of A. hydrophila was better than the free phage only at the first cycle and decreased upon reuse. The same was reported by Flood and Ashbolt, (49) which was inconsistent with that reported by Fattouh et al, (16) who recorded the phage activity for six successive cycles.…”

Section: Parahaemolyticus V Alginolyticus Andsupporting

confidence: 92%

“…(15) Immobilized phage could be particularly useful to create antimicrobial surfaces against pathogenic bacteria and was used in different applications. (16)(17)(18)(19) The aim of the present study was to estimate the counts of different groups of pathogenic bacteria such as Vibrio spp.…”

Section: Although Bacteriophages Werementioning

confidence: 99%

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Isolation and Characterization of some Bacteriophages and their Associated Bacteria in Sea Food: Phage-Host Interaction

Hassan

2011

Journal of High Institute of Public Health

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Background: Economic loss due to diseases is a major problem in aquaculture. A number of bacterial illnesses may arise from the consumption of sea food that has been contaminated at source or during the processing. This study aims to isolate and characterize some bacteriophages specific for the most common sea food pathogens in addition to their associated bacteria. The work also extended to study the host-phage interaction and the potential use of phages as antibacterial agents to control sea food infecting pathogens. Materials and methods: Estimation of Vibrio spp and Aeromonas spp. in addition to their associated phages in different sources of sea food such as fish (Tilapia sp.), clam (Tapes decussatns) and crab (Callinectes sapidus) was carried out. Results: Crab exhibited the highest counts (200 CFU/g) of Vibrio spp., while the highest counts of Vibriophages (1500 PFU/g) were detected in clam. Fish harbored the highest counts (30 CFU/g) and (3000 PFU/g) of Aeromonas spp. and Aeromonas phages, respectively. Three different phages (VPS1, APS2 and APS3) in addition to the isolated bacteria were characterized. Results showed that VPS1, APS2 and APS3 were different, which was confirmed by molecular characterization using RAPD-PCR and the protein profile. APS3 was entrapped into calcium alginate beads and tested as antibacterial agent against Aeromonas hydrophila. Efficiency of APS3 was superior to the free particles (p<0.05) and realized 2.7 fold decrease in bacterial growth rate. Phage seeded beads were recycled for 7 successive cycles. Their activity was reduced up on reuse. Recommendations: This study revealed that phages remain an excellent potential tool for control of bacterial pathogens. Further research and manipulation of the isolated phages could produce novel effective biocontrol agents of sea food infectious diseases in marine aquaculture systems

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Section: Parahaemolyticus V Alginolyticus Andsupporting

confidence: 94%

Section: Parahaemolyticus V Alginolyticus Andsupporting

confidence: 92%

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Isolation and Characterization of some Bacteriophages and their Associated Bacteria in Sea Food: Phage-Host Interaction

Hassan

2011

Journal of High Institute of Public Health

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“…Quantitatively, 20% of the φX174 or T4 infective viral particles in initial contact with the (PAH–HA) hydrogel were entrapped after 24 h, and the loading rates (evaluated in the linear, short-term part of the curves displayed in Figure a) were (0.62 ± 0.21) × 10 6 and (0.68 ± 0.22) × 10 6 viruses/h for φX174 and T4, respectively. Such invariance in the amount of entrapped viral particles with respect to phage geometry was also reported for H387 ( Siphoviridae ) and A511 phages ( Myoviridae ) and other coliphages loaded into alginate and polysaccharidic hydrogels. , In these previous studies, however, bacteria were immobilized into or at the hydrogel surface prior to phage production and incorporation in the matrix, whereas, here, the hydrogel surface was exposed to suspensions of viruses produced beforehand (Scheme ).…”

Section: Results and Discussionmentioning

confidence: 53%

“…Such invariance in the amount of entrapped viral particles with respect to phage geometry was also reported for H387 (Siphoviridae) and A511 phages (Myoviridae) and other coliphages loaded into alginate and polysaccharidic hydrogels. 58,59 In these previous studies, however, bacteria were immobilized into or at the hydrogel surface prior to phage production and incorporation in the matrix, whereas, here, the hydrogel surface was exposed to suspensions of viruses produced beforehand (Scheme 1).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Impacts of Mechanical Stiffness of Bacteriophage-Loaded Hydrogels on Their Antibacterial Activity

Francius

Cervulle

Clément

et al. 2021

ACS Appl. Bio Mater.

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The elaboration of novel and efficient hydrogel-based materials with antimicrobial properties requires a refined control of their defining physicochemical features, which includes mechanical stiffness, so as to properly mediate their antibacterial activity. In this work, we design hydrogels consisting of polyelectrolyte multilayer films for the loading of T4 and φX174 bacteria-killing viruses, also called bacteriophages. We investigate the anti-adhesion and bactericidal performances of this biomaterial against Escherichia coli, with a specific focus on the effects of chemical cross-linking of the hydrogel matrix which, in turn, mediates the hydrogel stiffness. Depending on the latter and on phage replication features, it is found that the hydrogels loaded with the bacteria-killing viruses make it possible both contactkilling (targeted bacteria are those adhered at the hydrogel surface) and release killing (planktonic bacteria are the targets) with ca. 20% to 80% efficiency after only 4 hours incubation at 25°C, as compared to cases where hydrogels are free of viruses. We further demonstrate the lack of dependence of virus diffusion within the hydrogel and of the maximal viral storage capacity on the hydrogel mechanical properties. In addition to the evidenced bacteriolytic activity of the phages loaded in the hydrogels, the antimicrobial property of the phages-loaded materials is shown to be partly controlled by the chemistry of the hydrogel skeleton and, more specifically, by the mobility of the peripheral free polycationic components, known for their ability to weaken and permeabilize membranes of bacteria, the latter then becoming 'easier' targets for the viruses.

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Effect of Coliphage Entrapment on its Interaction with the Host Bacterium

Cited by 2 publications

References 17 publications

Isolation and Characterization of some Bacteriophages and their Associated Bacteria in Sea Food: Phage-Host Interaction

Isolation and Characterization of some Bacteriophages and their Associated Bacteria in Sea Food: Phage-Host Interaction

Impacts of Mechanical Stiffness of Bacteriophage-Loaded Hydrogels on Their Antibacterial Activity

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