Effect of colloidal β‐cyclodextrins–Fe₃O₄ magnetic nanoparticles on the chemiluminescence enhancement of luminol–Ag(III) complex for rapid and sensitive determination of cysteine in human serum

Abstract

Colloidals solution of Fe3 O4 magnetic nanoparticles (MNPs), capped with β-cyclodextrins (β-CD) as inclusion complexes, were found to enhance the chemiluminescence (CL) intensity of the luminol-diperiodatoargentate(III) (DPA) system. On injection of cysteine into the luminol-DPA-β-CD-Fe3 O4 MNPs inclusion complexes system, the CL intensity is strongly enhanced. The enhanced CL signal is ascribed to the catalytic effect of Fe3 O4 MNPs capped with β-CD, which is assumed to stabilize the CL intermediate. Based on these findings, a rapid and sensitive assay was developed for the determination of cysteine in human serum. The effects of analytical variables on the CL signal were studied and optimized. Under the optimum conditions, the CL intensity was directly proportional to the concentration of cysteine in the range 8.0 × 10(-9) -1.0 × 10(-6) mol/L. The detection limit was 2.8 × 10(-9) mol/L (3 S(b) /m) and the relative standard deviation (RSD) for 10 replicate determinations of 1.0 × 10(-7) mol/L cysteine was 3.5%. The proposed method was applied to the sensitive determination of cysteine in human serum samples, and compared with the Ellman method with satisfactory results.

Sensitive determination of norepinephrine, epinephrine, dopamine and 5‐hydroxytryptamine by coupling HPLC with [Ag(HIO6)2]5−–luminol chemiluminescence detection

“…(Balikungeri et al, 1977). The Ag(III) complex was characterized by UV-visible spectra on a model 8453 spectrophotometer (Agilent, USA), exhibiting two absorb peaks at 252 and 362 nm, in agreement with the values reported earlier (Rezaei et al, 2012;Masse and Simon, 1982). The Ag(III) complex was used freshly daily.…”

“…Some studies have demonstrated that the oxidation of luminol by the Ag(III) complex can generate CL in alkaline, which has been used to quantify 6-mercaptopurine (Sun et al, 2013), dopamine (Xu et al, 2011), 10-hydroxycamptothecin , cysteine (Rezaei et al, 2012) and cortisol (Shi et al, 2009) by a flow-injection CL method in blood samples and pharmaceutical preparations. Some studies have demonstrated that the oxidation of luminol by the Ag(III) complex can generate CL in alkaline, which has been used to quantify 6-mercaptopurine (Sun et al, 2013), dopamine (Xu et al, 2011), 10-hydroxycamptothecin , cysteine (Rezaei et al, 2012) and cortisol (Shi et al, 2009) by a flow-injection CL method in blood samples and pharmaceutical preparations.…”

“…The Ag(III) complex ([Ag(HIO 6 ) 2 ] 5À ), with a square-planar coordination geometry around the metal center, is a powerful oxidizing agent. Some studies have demonstrated that the oxidation of luminol by the Ag(III) complex can generate CL in alkaline, which has been used to quantify 6-mercaptopurine (Sun et al, 2013), dopamine (Xu et al, 2011), 10-hydroxycamptothecin , cysteine (Rezaei et al, 2012) and cortisol (Shi et al, 2009) by a flow-injection CL method in blood samples and pharmaceutical preparations. The above methods could only detect a single compound.…”

Fabrication and characterization of Cu(OH)2/CuO nanowires as a novel sensitivity enhancer of the luminol–H2O2chemiluminescence system: determination of cysteine in human plasma

“…However, direct determination of cysteine in plasma was not successful. Therefore, according to Rezaei et al, 60 a deproteination process was carried out by using acetone and the standard addition was carried out by spiking known amounts of cysteine standard solution to plasma samples. For the release of cysteine which is bound to protein in plasma, 10.0 mL b-mercaptoethanol was added to 2.0 mL of the plasma.…”

One‐step synthesis of cationic gold nanoclusters with high catalytic activity on luminol chemiluminescence reaction

“…have been reported to enhance CL reactions. [ 15–25 ] Metal nanoclusters (NCs) are composed of several to dozens of metal atoms, and commonly have an ultra‐small size of < 2 nm. [ 26 ] Compared with metal nanoparticles, metal nanoclusters are smaller in size and have a larger specific surface area.…”