Fabrication and characterization of Cu(OH)2/CuO nanowires as a novel sensitivity enhancer of the luminol–H2O2chemiluminescence system: determination of cysteine in human plasma
Abstract:Cu(OH)2/CuO nanowires as a sensitivity enhancer in luminol–H2O2 chemiluminescence system and its application for the determination of cysteine.
“…The E g value of the CuO NPs was found to be 3.3 eV in this study. The feature of the plot indicated that it had a direct transition due to the linear absorption at the end of the plot [50]. The higher band gap energy of the CuO NPs than the reported value (1.9-2.1 eV) could be related to the quantum confinement effect that is the band gap increase with a reduction in particle size [51].…”
In recent years, the green chemistry based-approach for the synthesis of nanoparticles has shown tremendous promise as an alternative to the costly and environmentally unfriendly chemically synthesized nanoparticles. In this study, copper oxide nanoparticles (CuO NPs) were synthesized through a green approach using the water extract of papaya (Carica papaya L.) peel biowaste as reducing as well as stabilizing agents, and copper (II) nitrate trihydrate salt as a precursor. The structural properties, crystallinity, purity, morphology, and the chemical composition of as-synthesized CuO NPs were analyzed using different analytical methods. The analytical results revealed that the synthesized CuO was observed as spherical-like in particles with measured sizes ranging from 85–140 nm and has monoclinic crystalline phase with good purity. The Fourier transform infrared (FTIR) spectroscopic results confirmed the formation of the Cu-O bond through the involvement of the potential functional groups of biomolecules in papaya peel extract. Regarding photocatalytic activity, the green-synthesized CuO NPs were employed as a photocatalyst for the degradation of palm oil mill effluent (POME) beneath the ultraviolet (UV) light and results showed 66% degradation of the POME was achieved after 3 h exposure to UV irradiation. The phytotoxicity experiment using mung bean (Vigna radiata L.) seed also showed a reduction of toxicity after photodegradation.
“…The E g value of the CuO NPs was found to be 3.3 eV in this study. The feature of the plot indicated that it had a direct transition due to the linear absorption at the end of the plot [50]. The higher band gap energy of the CuO NPs than the reported value (1.9-2.1 eV) could be related to the quantum confinement effect that is the band gap increase with a reduction in particle size [51].…”
In recent years, the green chemistry based-approach for the synthesis of nanoparticles has shown tremendous promise as an alternative to the costly and environmentally unfriendly chemically synthesized nanoparticles. In this study, copper oxide nanoparticles (CuO NPs) were synthesized through a green approach using the water extract of papaya (Carica papaya L.) peel biowaste as reducing as well as stabilizing agents, and copper (II) nitrate trihydrate salt as a precursor. The structural properties, crystallinity, purity, morphology, and the chemical composition of as-synthesized CuO NPs were analyzed using different analytical methods. The analytical results revealed that the synthesized CuO was observed as spherical-like in particles with measured sizes ranging from 85–140 nm and has monoclinic crystalline phase with good purity. The Fourier transform infrared (FTIR) spectroscopic results confirmed the formation of the Cu-O bond through the involvement of the potential functional groups of biomolecules in papaya peel extract. Regarding photocatalytic activity, the green-synthesized CuO NPs were employed as a photocatalyst for the degradation of palm oil mill effluent (POME) beneath the ultraviolet (UV) light and results showed 66% degradation of the POME was achieved after 3 h exposure to UV irradiation. The phytotoxicity experiment using mung bean (Vigna radiata L.) seed also showed a reduction of toxicity after photodegradation.
“…(iii) When H 2 O 2 is added to the system, G4/MOFzyme would react with H 2 O 2 to produce a highly reactive hydroxyl radical OH · followed by the reaction with luminol anion and HO 2 – to facilitate the formation of luminol radicals and O 2 · – . Finally, luminol radicals react with O 2 · – to generate strong CL emission, which is beneficial for the accurate detection of target miRNAs and long-lasting CL imaging. , …”
Section: Resultsmentioning
confidence: 99%
“…(iii) When H 2 O 2 is added to the system, G4/MOFzyme would react with − to generate strong CL emission, which is beneficial for the accurate detection of target miRNAs and long-lasting CL imaging. 74,75 Applications to CL Printing. Due to the excellent catalytic ability of the G4/MOFzyme, we sought to apply it to other fields.…”
Here,
we report a double-amplified sensing platform for ultrasensitive
chemiluminescence (CL) miRNA detection in real patients’ blood
in which a hemin-bridged metal–organic framework (MOF) is employed
as a functional interface to boost the payload and catalysis of G-quadruplex
(G4) DNAzymes. Hemin is here used as the organic ligand for the MOF
synthesis, which endows the MOF with an intrinsic peroxidase-like
catalytic activity. Most importantly, the MOF surface provides a large
amount of binding sites for polymeric G4 DNAzymes that are produced
by miRNA-triggered rolling circle amplification reactions, and meanwhile,
the interfaced G4 DNAzymes on MOFs (G4/MOFzymes) display an about
100-fold higher catalytic activity than those in solution. By using
the G4/MOFzyme catalysts in the luminol/H2O2 CL system, the amplification detection of two acute myocardial infarction
(AMI)-related miRNAs (low to 1 fM seen with naked eyes) is achieved
in human serum with a smartphone as a portable imaging detector, which
provides a facile methodology for point-of-care (POC) diagnosis of
AMI. Compared with previous smartphone-based counterparts not requiring
sophisticated equipment, this new facile methodology shows both 6
orders of magnitude higher sensitivity and an ∼50-fold longer
duration for CL miRNA imaging. These unique features allow our developed
G4/MOFzymes to be further employed as a novel luminescent ink for
printing commonly used patterns.
“…The features of peaks at 587, 1000 and 3576 cm −1 are related to the C-O stretching vibration and C-O-H bending vibration, as well as O-H stretching vibration proving the successful synthesis of coated Cu(OH) 2 with Fe 3 O 4 @SiO 2 . In addition, 1500 cm −1 belongs to H-O-H bending vibrations of H 2 O adsorbed onto the FSCNWs lattice (figure 5) [35,46,47]. Figure 5FT-IR spectra of Fe 3 O 4 @SiO 2 /Cu(OH) 2 .…”
In this study, green synthesis of modified Cu(OH)
2
nanowires by Fe
3
O
4
@SiO
2
core–shell nanospheres was easily performed via chemical reduction. In other words, the direct coating of Cu(OH)
2
on Fe
3
O
4
@SiO
2
was successfully realized without the extra complicated procedures. Various concentrations of synthesized nanocomposites were tested on pathogenic and nosocomial bacteria. In this study, the structural information and characterization of Fe
3
O
4
@SiO
2
/Cu(OH)
2
nanowires (FSCNWs) were obtained using FE-SEM, FT-IR, EDX and X-ray diffraction. This nanocomposite can effectively kill important infectious bacteria, including
Staphylococcus aureus
,
Escherichia coli
,
Staphylococcus saprophyticus
,
Pseudomonas aeruginosa
and
Klebsiella pneumoniae
. Studies have shown that FSCNW nanocomposites affect common antibiotic-resistant bacteria. This result confirms the function of FSCNW as an effective, beneficial and environmentally friendly antibacterial agent that can used in a wide range of applications in medicine. FSCNWs can be separated conveniently from bacteria-containing solutions using a magnet. Compared with nanocomposites based on other metals such as silver and gold, the use of FSCNWs in water treatment has been recommended because of the precursor of copper for its low price and less toxicity. In addition to its special properties such as mild reaction conditions, green synthesis methods, admissible magnetic properties, easy separation, high antibacterial activity and beneficial efficiency.
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