Effect of concanavalin A on the activity of membrane-bound and detergent-solubilized Mg2+-ATPase

Ohtsu, Yoshiaki; Tsuchida, Kenji; Suzuki, Yu; Ohnishi, Takayuki

doi:10.1016/0005-2736(82)90239-5

Biochimica et Biophysica Acta (BBA) - Biomembranes

1982

DOI: 10.1016/0005-2736(82)90239-5

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Effect of concanavalin A on the activity of membrane-bound and detergent-solubilized Mg2+-ATPase

Yoshiaki Ohtsu

Kenji Tsuchida

Yu Suzuki

et al.

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1984

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Cited by 5 publications

(1 citation statement)

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“…Also, two independent groups found (9,10) to final concentrations of 1 mM, 1% (vol/vol), and 6 mM, respectively. Incubations at 37°C for 72 hr caused extensive precipitation with poor yields of CF1 after repurification.…”

mentioning

confidence: 97%

Partial deglycosylation of chloroplast coupling factor 1 (CF1) prevents the reconstitution of photophosphorylation.

Maione¹,

Jagendorf²

1984

Proc. Natl. Acad. Sci. U.S.A.

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Treatment of spinach chloroplast coupling factor 1 (CF1) with a mixture of glycosidases resulted in the removal of =75% of the carbohydrate associated with the enzyme. The ATPase of CF1 was not activated by this treatment nor were its heat-activated or methanol-dependent ATPase activities inhibited. The deglycosylated enzyme, however, was unable to catalyze photophosphorylation when recoupled with CF,-depleted thylakoids. The glycosidase-treated protein competed with native CF1 for specific binding sites on the depleted membranes and was able to reconstitute proton uptake in uncoupled thylakoids. The inhibition of photophosphorylation could not be overcome with added 6 subunit. We conclude that deglycosylated CF1 was unaffected in its ability to bind to the membrane sector of the chloroplast proton-pumping ATPase (CFO) but was altered in some property essential for photophosphorylation but not ATPase activity.

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mentioning

confidence: 97%

Partial deglycosylation of chloroplast coupling factor 1 (CF1) prevents the reconstitution of photophosphorylation.

Maione¹,

Jagendorf²

1984

Proc. Natl. Acad. Sci. U.S.A.

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Partial purification and characterization of rabbit-kidney brush-border (Ca2+ or Mg2+)-dependent adenosine triphosphatase

Mörtl

Busse

Bartel

et al. 1984

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Characterization of Mg2+-ATPase from slow-twitch muscle membranes

Szyja

Wrzosek

Korczak

et al. 1987

International Journal of Biochemistry

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Effect of concanavalin A on the activity of membrane-bound and detergent-solubilized Mg2+-ATPase

Cited by 5 publications

References 13 publications

Partial deglycosylation of chloroplast coupling factor 1 (CF1) prevents the reconstitution of photophosphorylation.

Partial deglycosylation of chloroplast coupling factor 1 (CF1) prevents the reconstitution of photophosphorylation.

Partial purification and characterization of rabbit-kidney brush-border (Ca2+ or Mg2+)-dependent adenosine triphosphatase

Characterization of Mg2+-ATPase from slow-twitch muscle membranes

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