Effect of Concanavalin A on the Functional Activity of Hemolytic Antibody

Abstract

Concanavalin A (Con A), either in solution or insolubilized by covalent binding to Sepharose 4B, can inhibit the ability of fluid phase 19S, but not 7S, anti-Forssman antibody to sensitize sheep red cells (E) toward lysis by excess guinea pig complement. The efficiency of 19S antibody is unaffected when E are treated with Con A before sensitization or when antibody sensitized cells (EA) are exposed to the lectin before complement is added. Although whole complement activity is retained on a solumn of Con A-Sepharose, cell bound lectin did not act as a complement fixing antibody. Consistent with this result, there was no difference in the amount of C1 fixed by E and E-Con A, or by EA and EA-Con A.

Deficient Serum Bactericidal Activity Against Escherichia Coli in Patients with Cirrhosis of the Liver

“…J.) (16). IgG from the second peak was separated from contaminating IgA by use of DEAE-Sephadex (Phannacia Fine Chemicals) (17).…”