Effect of Consumed Carbon to Nitrogen Ratio on Mycelial Morphology and Arachidonic Acid Production in Cultures of Mortierella alpina.

Production of microbial oil with high oleic acid content by Trichosporon capitatum

“…For example, oleic acid content went up from 69.5% to 84.0% when C/N molar ratio increased from 82 to 147 whereas linoleic acid content declined from 18.2% to 4.0%. Obviously, this observation supports the previous report that C/N molar ratio affects the fatty acid composition of microbial lipid greatly [26]. It has been reported that divalent metal ions could bind to malic enzyme, which has been recognized as a key enzyme for lipid accumulation, thus influencing the structural integrity of the enzyme [27].…”

“…Temperature affects not only cell growth but also lipid formation [26]. As depicted in Table 3, biomass increased with increasing temperature up to 28°C and further rise in temperature resulted in a decline in biomass.…”

“…A poor lipid yield was achieved on sucrose, while xylose and lactose were unfavorable carbon sources for lipid production with T. capitatum AS 2.1385. Since the carbon-tonitrogen (C/N) molar ratio plays a crucial role in the lipid production of oleaginous microorganisms [26], the effect of C/N molar ratio on the fermentation was further examined (Table 1). Both the biomass and the lipid content increased slowly with increasing C/N molar ratio from 82 to 98, followed by a rapid rise up to 147, at which the lipid yield reached the maximum (5.2 g/L).…”

Fermentation Characteristics of Mortierella alpina in Response to Different Nitrogen Sources

“…Lipid accumulation is only favored by a fixed range of carbon to nitrogen (C/N) ratio, and C/N ratio also influences ARA production in the culture of M. alpina [26]. The optimal C/N ratio for ARA production was around 15-20 in a culture of M. alpina CBS 754.68 [27]. When the C/N ratio was higher than 20, cell growth was inhibited and ARA decreased due to nitrogen limitation [13].…”

Enhancing arachidonic acid production by Mortierella alpina ME-1 using improved mycelium aging technology

“…As a precursor of prostaglandins, thromboxane, prostacyclin, and leucotrienes, it owns various physiological functions [1,2] and has been found wide application in medicine, pharmacology, cosmetics, food industry, agriculture, and other fields [3]. Production of ARA by cultivation of Mortierella fungi, which are thought to be the most prominent ARA source [4], has been widely reported [5][6][7][8], focusing on the optimization of cell morphology, dissolved oxygen concentration, and medium compositions.…”