Effect of copper exposure on gene expression profiles in Chlamydomonas reinhardtii based on microarray analysis

Jamers, An; Ven, Karlijn van der; Moens, Lotte; Robbens, Johan; Potters, Geert; Guisez, Yves; Blust, Ronny; Coen, Wim De

doi:10.1016/j.aquatox.2006.09.002

Cited by 79 publications

(48 citation statements)

References 31 publications

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“…Finally, our study demonstrates that valuable results can be obtained through a large scale heterologous microarray approach when homologous chips are not available. Possibility of cross species cDNA hybridization at subdivision or closer levels could be a beneficial tool for revealing gene expression in various marine organisms of other taxa starting from the chips already available in molluscs (Jenny et al, 2007), algae (Jamers et al, 2006) and crustaceans (Wang et al, 2006). Table 1 The 15 most abundant gene ontology categories associated with the 1,278 analyzed genes Table 2 The four most highly represented biological processes (GO) associated with the differentially regulated gene.…”

Section: Resultsmentioning

confidence: 99%

“…There is indeed a consensus that regulation of gene expression is a fundamental mechanism underlying the complex processes of cell differentiation and morphogenesis during ontogenesis of an organism. Recently, microarrays have dramatically accelerated many types of investigations in marine organisms, been especially used for comparative genomic hybridization (Fish; Moriya et al, 2004 and2007) and for monitoring the expression levels of thousands of genes simultaneously in order to study the effects of certain treatments (Algae; Jamers et al, 2006), diseases (Crustaceans; Wang et al, 2006) and transgenesis manipulation (Mori et al, 2007) on transcriptome. Over the last two decades, studies based on whole-body or tissue-specific microarray approaches have investigated gene expression profiles during embryogenesis in several species (Drosophila, White et al, 1999;Xenopus, Baldessari et al, 2005;mouse, Tanaka et al, 2000) including fish (zebrafish, Ton et al, 2002;sea bream, Sarropoulou et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Gene Expression Patterns During the Larval Development of European Sea Bass (Dicentrarchus Labrax) by Microarray Analysis

et al. 2008

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Abstract:During the larval period, marine teleosts undergo very fast growth and dramatic changes in morphology, metabolism, and behavior to accomplish their metamorphosis into juvenile fish. Regulation of gene expression is widely thought to be a key mechanism underlying the management of the biological processes required for harmonious development over this phase of life. To provide an overall analysis of gene expression in the whole body during sea bass larval development, we monitored the expression of 6,626 distinct genes at 10 different points in time between 7 and 43 days post-hatching (dph) by using heterologous hybridization of a rainbow trout cDNA microarray. The differentially expressed genes (n = 485) could be grouped into two categories: genes that were generally up-expressed early, between 7 and 23 dph, and genes up-expressed between 25 and 43 dph. Interestingly, among the genes regulated during the larval period, those related to organogenesis, energy pathways, biosynthesis, and digestion were over-represented compared with total set of analyzed genes. We discuss the quantitative regulation of whole-body contents of these specific transcripts with regard to the ontogenesis and maturation of essential functions that take place over larval development. Our study is the first utilization of a transcriptomic approach in sea bass and reveals dynamic changes in gene expression patterns in relation to marine finfish larval development.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Gene Expression Patterns During the Larval Development of European Sea Bass (Dicentrarchus Labrax) by Microarray Analysis

et al. 2008

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“…An induction of CrGPX1 expression was observed under sulfur starvation (Zhang et al 2004) and CrGPX3 and CrGPX4 transcript levels were shown to be upregulated during manganese deficiency probably due to the resulting oxidative stress (Allen et al 2007). Similarly, the mRNA levels of CrGPX5/GPXH, which appear to be the most highly expressed GPX on the basis of EST abundance (Table 2), were increased in Chlamydomonas cells exposed to copper stress (Jamers et al 2006).…”

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confidence: 93%

“…In Arabidopsis, the accumulation of PRX-Q mRNA is increased under high light or oxidative stress and decreased by salt stress, ascorbate, or under low light (Horling et al , 2003. The transcript levels of Chlamydomonas PRX-Q, CrPRX6, was reported to be induced in Chlamydomonas cells exposed to copper stress ( Jamers et al 2006) but also during sulfur starvation (Zhang et al 2004). …”

Section: Expression Of Peroxiredoxinsmentioning

confidence: 99%

The Peroxiredoxin and Glutathione Peroxidase Families in Chlamydomonas reinhardtii

et al. 2008

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Thiol/selenol peroxidases are ubiquitous nonheme peroxidases. They are divided into two major subfamilies: peroxiredoxins (PRXs) and glutathione peroxidases (GPXs). PRXs are present in diverse subcellular compartments and divided into four types: 2-cys PRX, 1-cys PRX, PRX-Q , and type II PRX (PRXII). In mammals, most GPXs are selenoenzymes containing a highly reactive selenocysteine in their active site while yeast and land plants are devoid of selenoproteins but contain nonselenium GPXs. The presence of a chloroplastic 2-cys PRX, a nonselenium GPX, and two selenium-dependent GPXs has been reported in the unicellular green alga Chlamydomonas reinhardtii. The availability of the Chlamydomonas genome sequence offers the opportunity to complete our knowledge on thiol/selenol peroxidases in this organism. In this article, Chlamydomonas PRX and GPX families are presented and compared to their counterparts in Arabidopsis, human, yeast, and Synechocystis sp. A summary of the current knowledge on each family of peroxidases, especially in photosynthetic organisms, phylogenetic analyses, and investigations of the putative subcellular localization of each protein and its relative expression level, on the basis of EST data, are presented. We show that Chlamydomonas PRX and GPX families share some similarities with other photosynthetic organisms but also with human cells. The data are discussed in view of recent results suggesting that these enzymes are important scavengers of reactive oxygen species (ROS) and reactive nitrogen species (RNS) but also play a role in ROS signaling. L IFE in an oxygen-rich environment has to deal with the danger of oxidative stress. During normal cell metabolism, reactive oxygen species (ROS) and reactive nitrogen species (RNS) are constantly produced, essentially by respiratory and photosynthetic electron transfer chains. These highly reactive molecules can react with many cell components and damage DNA, proteins, and lipids. Thus, their concentration has to be strictly controlled. For this purpose, aerobic organisms are equipped with nonenzymatic (ascorbate, glutathione, tocopherol, and carotenoid) or enzymatic (catalase, ascorbate peroxidase, superoxide dismutase, glutathione peroxidase, and peroxiredoxin) antioxidant systems to remove ROS from the cells. To control their concentrations, ROS and RNS have to be sensed. It has been established in many organisms that ROS, and especially hydrogen peroxide, are signaling molecules that diffuse across membranes and induce specific signal transduction pathways. Furthermore, ROS can also be produced on purpose by cells in response to several stimuli to function as second messengers inside the cell. Finally, ROS and RNS can control enzyme activities by triggering several post-translational modifications such as disulfide bond formation, thiol oxidation to sulfenic/sulfinic/sulfonic acid, glutathionylation, nitrosylation, or carbonylation.Thiol/selenol peroxidases have emerged, during recent years, as important scavengers of ROS/RNS but they ...

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“…html). At this time, a number of microarrays have been used to interrogate changes in response to environmental factors (Ledford et al, 2004(Ledford et al, , 2007Jamers et al, 2006;Mus et al, 2007;Nguyen et al, 2008;Simon et al, 2008;Yamano et al, 2008;Mustroph et al, 2010). These microarrays could not cover all genes in the genome, but more recently, massively parallel cDNA sequencing approaches were applied to C. reinhardtii, overcoming the shortcomings of microarrays (González-Ballester et al, 2010).…”

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confidence: 99%

Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism

et al. 2010

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Like many microalgae, Chlamydomonas reinhardtii forms lipid droplets rich in triacylglycerols when nutrient deprived. To begin studying the mechanisms underlying this process, nitrogen (N) deprivation was used to induce triacylglycerol accumulation and changes in developmental programs such as gametogenesis. Comparative global analysis of transcripts under induced and noninduced conditions was applied as a first approach to studying molecular changes that promote or accompany triacylglycerol accumulation in cells encountering a new nutrient environment. Towards this goal, high-throughput sequencing technology was employed to generate large numbers of expressed sequence tags of eight biologically independent libraries, four for each condition, N replete and N deprived, allowing a statistically sound comparison of expression levels under the two tested conditions. As expected, N deprivation activated a subset of control genes involved in gametogenesis while down-regulating protein biosynthesis. Genes for components of photosynthesis were also down-regulated, with the exception of the PSBS gene. N deprivation led to a marked redirection of metabolism: the primary carbon source, acetate, was no longer converted to cell building blocks by the glyoxylate cycle and gluconeogenesis but funneled directly into fatty acid biosynthesis. Additional fatty acids may be produced by membrane remodeling, a process that is suggested by the changes observed in transcript abundance of putative lipase genes. Inferences on metabolism based on transcriptional analysis are indirect, but biochemical experiments supported some of these deductions. The data provided here represent a rich source for the exploration of the mechanism of oil accumulation in microalgae.

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Effect of copper exposure on gene expression profiles in Chlamydomonas reinhardtii based on microarray analysis

Cited by 79 publications

References 31 publications

Gene Expression Patterns During the Larval Development of European Sea Bass (Dicentrarchus Labrax) by Microarray Analysis

Gene Expression Patterns During the Larval Development of European Sea Bass (Dicentrarchus Labrax) by Microarray Analysis

The Peroxiredoxin and Glutathione Peroxidase Families in Chlamydomonas reinhardtii

Changes in Transcript Abundance in Chlamydomonas reinhardtii following Nitrogen Deprivation Predict Diversion of Metabolism

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