Effect of CPP-ACP paste on tooth mineralization: an FE-SEM study

Abstract

Milk and milk products, such as cheese, have been shown to exhibit anticariogenic properties in human and animal models. CPP-ACP shows an anti-caries effect by suppressing demineralization, enhancing remineralization, or possibly a combination of both. The purpose of this study was to evaluate the effect of CPP-ACP paste on demineralization by observing the treated tooth surface using an FE-SEM. The specimens were prepared by cutting enamel and dentin of bovine teeth into blocks. A few specimens were stored in 0.1 M lactic acid buffer solution for 10 min and then in artificial saliva (negative control). The remaining specimens were stored in a 10 times-diluted solution of CPP-ACP paste or a placebo paste containing no CPP-ACP for 10 min, followed by 10 min immersion in a demineralizing solution (pH = 4.75, Ca) twice a day before storage in artificial saliva. After treatment of the specimens for 3, 7, 21 and 28 days, they were fixed in 2.5% glutaraldehyde in cacodylate buffer solution, dehydrated in ascending grades of tert-butyl alcohol, and then transferred to a critical-point dryer. The surfaces were coated with a thin film of Au in a vacuum evaporator, and were observed under field emission-scanning electron microscopy (FE-SEM). The SEM observations revealed different morphological features brought about by the various storage conditions. Demineralization of the enamel and dentin surfaces was more pronounced with the longer test period in the control and negative control specimens. On the other hand, enamel and dentin specimens treated with CPP-ACP paste revealed slight changes in their morphological features. From the morphological observations of the enamel and dentin surfaces, it could be considered that the CPP-ACP paste might prevent demineralization of the tooth structure.

Efeito do uso de agentes clareadores com cálcio na microdureza do esmalte dental

“…Além disso, o tipo de cálcio na composição do agente clareador testado (gluconato de cálcio) não é o mesmo CPP-ACP encontrado na maioria dos estudos 28,29 . Em um estudo que usou CPP-ACP diluído no agente clareador, foi encontrada diminuição na microdureza do esmalte 30 .…”

Comparison of the newer preventive therapies on remineralisation of enamel in vitro

“…This suggests the preventive role of CPP-ACP in enamel demineralisation (Yamaguchi et al, 2007). Ferrazzano et al (2007), used the weight loss of the samples to determine demineralisation in their study.…”

“…A study using sonic velocity to quantify bovine enamel mineralisation reported that using a 1:10 dilution of Tooth Mousse enhances the mineralisation of enamel under acidic conditions (0.1 M buffered lactic acid) for 10 minutes twice a day for 10 days (Yamaguchi et al, 2007).…”

Role of Casein Phosphopeptide–amorphous Calcium Phosphate (Cpp–acp) in Prevention of Dental Caries: A Review.

“…Enamel microabrasion together with prolonged use of CPP-ACP based paste is useful for treating white spot enamel lesions. 21 Method of application of topical cream: It can be applied directly with clean finger onto the teeth, smeared over all surfaces, and left in place to slowly dissolve overnight. Any material that is swallowed is completely safe and will contribute toward dietary calcium.…”