Effect of Cryopreservation Conditions on Phenotypic Profile and Immunomodulating Activity of Fetal Liver Cells

Leonova, L. A.; Bondarovich, Nikolay A.; Ostankov, Maksim V.; Goltsev, A. M.

doi:10.15407/cryo29.03.266

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…Відомо, що ступінь впливу факторів кріоконсервування на біологічний об'єкт визначається умовами реалізації процесу заморожування-відігрівання [97]. Незважаючи на те що кріоконсервування і ліофілізація є ‚агресивними‛ відносно структурних і функціональних харак-теристик клітин, ці процеси залишаються до теперішнього часу найбільш оптимальними методичними підходами до їх зберігання.…”

Section: обговоренняunclassified

Cord Blood as a Corrector of Hematological and Cytomorphological Characteristics of Lymphohemopoietic Organs in Experimental Atopic Dermatitis

Koval,

Bondarovych,

Goltsev

2023

Innov Biosyst Bioeng

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Background. Atopic dermatitis (AD) is an inflammatory skin disease caused by a shift in the interaction between cells of the immune system and the skin. Steroid anti-inflammatory drugs used to treat AD often lead to numerous side effects. Therefore, the development of new natural multifunctional preparations with immunomodulatory activity is of great importance. Objective. To determine the changes in indices of the leukocytes cluster of peripheral blood and determine the peculiarities of cytomorphological changes in the spleen and lymph nodes (LN) of rats with induced AD before and after administration of cryopreserved (cHCBL) and lyophilized (lHCBL) forms of human cord blood leukoconcentrate. Methods. The experiments were conducted using 6-month-old Wistar rats. AD was induced by topically applying a 5% alcohol-acetone solution of dinitrochlorobenzene (DNCB) to the skin of the rats' back for 21 days. Each form of the preparation (cHCBL and lHCBL) was administered intraperitoneally in a 0.5 ml dose containing 5´106 cells, one day after the final DNCB treatment. The following parameters were assessed in the blood: leukocyte count and the neutrophil-to-lymphocyte ratio (N/L index). Сytomorphological characteristics were determined in the spleen and inguinal LN. Results. In AD, specific changes in the cytomorphological characteristics of the lymphohematopoietic complex (LHС) structures, namely the spleen and LN, were observed alongside alterations in the leukocyte cluster indices. The most notable changes in the spleen included the dominance of the white pulp over the red pulp and the prevalence of follicles of stages II and III of development in the LN. Animals with AD displayed pronounced leukocytosis and an increased N/L index in their blood. The study demonstrates the positive corrective effects of cHCBL and lHCBL on the morphological characteristics of LHC and the specified blood parameters. Conclusions. The effectiveness of the cHCBL and lHCBL use for correcting indices of leukocytes cluster of peripheral blood and the structural organization of lymphohemopoietic organs in the development of AD have been proven.

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Section: обговоренняunclassified

Cord Blood as a Corrector of Hematological and Cytomorphological Characteristics of Lymphohemopoietic Organs in Experimental Atopic Dermatitis

Koval,

Bondarovych,

Goltsev

2023

Innov Biosyst Bioeng

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Modern approach to the treatment of atopic dermatitis with preserved fetal liver cells (experimental study)

Leonova¹,

Bondarovych²,

Ostankov³

et al. 2021

PUMTS

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One of the urgent problems of modern dermatology is atopic dermatitis (AD), which has multifactorial pathogenesis, the significant prevalence of the disease, the increased frequency of the complicated course, the lack of radical methods of therapy. The expediency to use cryopreserved fetal liver cells (cFLCs) for the treatment of AD is proved by a wide range of produced by them biologically active substances with immunomodulatory and anti-inflammatory activity. Disclosure of the mechanisms of the therapeutic action of biotherapeutic drugs in AD provides for the determination of the state of the cellular and humoral links of the immune system (IS). In this regard, the aim of the work was to assess the effectiveness of cFLCs injection by characteristic clinical and immunological parameters in rats with AD. The results of the study in rats with AD revealed disorders in the IS, manifested in a decrease in the total number of T-lymphocytes and their subpopulations in the spleen, in an increase in the level of circulating immune complexes and a number of immunoglobulins in the blood serum, and in a decrease in the phagocytic activity of the peritoneal cavity cells. Therapy with cFLCs, in contrast to the standard treatment with prednisolone, significantly improves the therapeutic effect, which is demonstrated by the restoration of the parameters of the cellular and humoral links of the immune system in animals with AD. The amplifying effect of the combined use of cFLCs and prednisolone on a number of parameters of the immune system in AD was shown.

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Effect of Cryopreservation Conditions on Phenotypic Profile and Immunomodulating Activity of Fetal Liver Cells

Cited by 2 publications

References 14 publications

Cord Blood as a Corrector of Hematological and Cytomorphological Characteristics of Lymphohemopoietic Organs in Experimental Atopic Dermatitis

Cord Blood as a Corrector of Hematological and Cytomorphological Characteristics of Lymphohemopoietic Organs in Experimental Atopic Dermatitis

Modern approach to the treatment of atopic dermatitis with preserved fetal liver cells (experimental study)

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