Effect of Cryopreservation on Rat Epididymis - A Histological Analysis

Kedam, Thyaga Raju; Katepogu, Kamala

doi:10.4172/2254-609x.100025

Cited by 2 publications

References 30 publications

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Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Lima

Soares

Stalker

et al. 2021

Zygote

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Summary This study aimed to describe the viability of domestic feline spermatozoa after epididymal tail vitrification. For this, 10 pairs of testis–epididymis complexes were used. The epididymal tails were vitrified using the solid-surface vitrification (SSV) method, in which two vitrification media containing ethylene glycol (EG) 40% or glycerol (GLY) 40% were tested. Vitrification with the presence of EG resulted in better results for all sperm motility parameters (motility, vigour and CASA) compared with GLY (P < 0.05). There were no statistical differences for sperm viability and acrosome integrity, plasma membrane integrity, or overall health of morphologically normal sperm before or after vitrification among experimental groups. In conclusion, epididymal tail vitrification appears to be a suitable method for long-term storage of cat sperm, especially if the procedure is performed with EG as the cryoprotectant.

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Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Lima

Soares

Stalker

et al. 2021

Zygote

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Biobanking and Use of Epididymal Sperm – An Alternative for Wildlife Germplasm Conservation

Rodrigues Silva,

Maria da Silva,

Parente dos Santos

et al. 2024

Veterinary Medicine and Science

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Wildlife has been strongly threatened both by climate changes, which directly impact their habitats, and by human action, which promotes deforestation or unrestrained poaching. While in situ conservation strategies such as habitat preservation are often the best way to preserve biodiversity, other rescue strategies such as germplasm conservation are also necessary to facilitate the continuation of natural processes of evolution. Thus, the recovery of spermatozoa from the tail of the epididymis of endangered or valuable individuals appears as an option for the preservation of genetic material, mainly in those individuals that die suddenly, or as an alternative for those species in which techniques for ejaculate collection have not yet been established. In this chapter, the basic concepts of epididymal physiology in some wild mammals will be demonstrated. Next, the recovery techniques and analyzes that allow the characterization of this gamete will be presented, as well as the different methods proposed for the refrigeration, freezing, and applications of epididymal spermatozoa in different species. Then, important concepts will be discussed regarding how this technology can be added to the efforts for the formation of biobanks for the conservation of wild mammals with biologically valuable genotypes.

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Effect of Cryopreservation on Rat Epididymis - A Histological Analysis

Cited by 2 publications

References 30 publications

Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Epididymal tail solid-surface vitrification as an effective method for domestic cat sperm cryobanking

Biobanking and Use of Epididymal Sperm – An Alternative for Wildlife Germplasm Conservation

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