Effect of Cryopreservation on Sperm Quality and Fertility

Lemma, Alemayehu

doi:10.5772/16563

Cited by 61 publications

(68 citation statements)

References 91 publications

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“…In general, semen cryopreservation protocols use freezing curves ranging from 10 to 100°C/min, resulting in good survival rates after thawing. Moreover, the freezing rate depends on the method of processing as well as of storage (Lemma 2011). The rate of temperature drop was found doi: 10.17221/23/2016-CJAS to be most critical over the specific temperature range of 0-5°C when motility was evaluated later.…”

Section: Discussionmentioning

confidence: 99%

“…The extent of spermatozoa damage caused by cold shock depends on temperature, however on the rate of temperature decrease as well. Generally, the higher the cooling rate, the more serious the sperm damage (Lemma 2011) determining the subsequent post-thaw spermatozoa motility (Andrabi 2007). Thus the result of sperm cryopreservation is also affected by the type and course of freezing curve .…”

Section: Introductionmentioning

confidence: 99%

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Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

Czech J. Anim. Sci.

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ABSTRACT:The objective was to determine effects of equilibration length and freezing curve type as well as their interactions on motility and live spermatozoa proportion in bull sperm after thawing. The ejaculates of 6 sires were repeatedly collected. Fresh semen was diluted with one extender and divided into 3 groups equilibrated for 30, 120, and 240 min. Subsequently, half straws of each group were frozen using standard 3-phase or 2-phase freezing curve differing in the rate of temperature decrease. Th e spermatozoa motility (M) was evaluated immediately after thawing and at 30, 60, 90, and 120 min of thermodynamic test (TDT). Live spermatozoa proportion was evaluated after thawing and at the end of TDT. Average of spermatozoa motility (AM), decrease of spermatozoa motility (MD), average proportion of live spermatozoa (ALS), and decrease of live spermatozoa proportion (DLS) through the TDT were calculated. Significant inter-sire differences in AM (0.45-17.0%; P < 0.05-0.01), MD (0.76-12.57%; P < 0.05-0.01), and ALS (0.99-23.8%; P < 0.01) were detected. The longest equilibration ensured the highest M during TDT and AM (+2.72 and +4.58%; P < 0.05-0.01), however higher MD (+4.06%; P < 0.01) compared to standard length as well. Straws freezed using 2-phase curve achieved higher M through TDT, AM (+7.3%; P < 0.01) as well as ALS (+11.77%; P < 0.01). The 2-phase curve presented higher M compared to the 3-phase freezing curve within all equilibration lengths. Significant differences in AM, MD,; P < 0.05-0.01) between equilibration length vs freezing curve interactions were determined. Results document the importance of equilibration length, freezing curve, and their interaction effect on live spermatozoa proportion and sperm motility after thawing as well as necessity of individual conditions for bulls semen processing and insemination doses production.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Doležalová¹,

Stádník²,

Biniová³

et al. 2016

Czech J. Anim. Sci.

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“…On the other side, crystallization is archenemy for this parameter. Therefore, it is necessary to evaluate the semen in terms of plasma and acrosomal membrane integrity in addition to motility (22). In researches (1, 2, 4, 25), PMAI of cryopreserved bull semen was found at around 60%.…”

Section: Discussionmentioning

confidence: 99%

Effects of boron added bull semen extender on post-thaw spermatological parameters

Tirpan¹,

Gürler²,

Olğaç³

et al. 2018

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this research, post-thaw effects of bull semen extenders including different amounts of boron (sodium pentaborate) on total motility (TMOT), progressive motility (PMOT), kinetic parameters of spermatozoa (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plasma membrane and acrosome integrity (PMAI) and DNA fragmentation index (DFI) were evaluated. Eight ejaculates were examined from four different bulls. Each ejaculate was split in to five aliquots and extended with standard Tris extender (Control) and 0.4 g boron with 0.5 g glucose (Group 1) 0.5; 0.7; 0.9 g boron without glucose (Group 2, Group 3 and Group 4 respectively) were used as experimental groups. After thawing TMOT, PMOT, kinetic traits of spermatozoa, PMAI and DFI were determined. According to motility and progressive motility (66.04%, 50.79%), Group 1 had higher values than the others, but differences between groups were not statistically significant (P>0.05). The highest values of VSL, LIN, STR and WOB were determined (28.15 µm/s, 42.98%, 71.69%, 59.71%) in Group 1 (P<0.05). While other kinetic traits of spermatozoa were higher numerically in Group 1 than the other groups, differences between groups were not statistically significant (P>0.05). The highest PMAI value was determined as 60.84% in Group 1 (P>0.05). The DFI rates were found similar in all control and experimental groups (P>0.05). In conclusion, it was observed that the boron (sodium pentaborate) has positive effects on post-thaw movement and structural traits. In addition, boron has no detrimental effect on DNA fragmentation index in semen freezing. Thus, boron could be used as an alternative semen extender compound.Keywords: Bull semen, CASA, flow-cytometry, frozen semen, sodium pentaborate. Boğa

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“…It is universally accepted that cryopreservation is also known to be detrimental to sperm function and fertility even with the most up to date techniques (Lemma, 2011). Generally, sperm viability is decreased by 50%, whereas fertilizing capacity is affected by a factor of sevenfold after cryopreservation (Lessard et al, 2000).…”

Section: Semen Qualitymentioning

confidence: 99%

Effect of long term storage in LN2 on bacterial load and preservability of semen in Murrah bulls

Meena

Raina

Bhakat

et al. 2015

IJAR

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Successful preservation of semen for longer period can be achieved by maintaining the viability of spermatozoa from its collection till its use for insemination. Therefore, we perceived the idea to determine the effect of long term storage of cryopreserved buffalo semen on sperm quality, bacterial load and fertility. Twenty years semen samples (1981)(1982)(1983)(1984)(1985)(1986)(1987)(1988)(1989)(1990)(1991)(1992)(1993)(1994)(1995)(1996)(1997)(1998)(1999)(2000) from twenty bulls and the data on conception rates of these bulls were collected from record room at ABRC, ICAR-NDRI, Karnal. The data was analyzed using least square analysis. The differences in individual motility percent in the semen between estimated initially at the time of freezing and estimates after storage (at the time of evaluation) were 6.49, 14.09, 13.13 and 6.02 percent, respectively. Even after long term storage (up to 20 years) there were little changes in the sperm motility percent. Changes in non-eosinophilic sperm count, sperm abnormalities, HOST and acrosome status, were less up to 20 years storage of semen in liquid nitrogen and the differences were non-significant between different years of storage semen in liquid nitrogen. In similar fashion the microbial load in semen was decreased with the increased storage period of semen in liquid nitrogen, but the differences were non significant. The differences in sperm motility percent between 37 0 C and room temperature were not significant, but the temperature and incubation period significantly (P<0.01) influenced motility percent estimates. Whereas, temperature-incubation period interaction was not found to be significant. The conception rate and sperm motility was highly and positively correlated (r=0.67 and 0.55) with sperm oocyte attachment. It can be concluded that there was little change occurred in semen quality even after twenty years of storage in liquid nitrogen without affecting fertility of semen.

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Effect of Cryopreservation on Sperm Quality and Fertility

Cited by 61 publications

References 91 publications

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

Effects of boron added bull semen extender on post-thaw spermatological parameters

Effect of long term storage in LN2 on bacterial load and preservability of semen in Murrah bulls

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