Effect of cryopreservation on the properties of human endometrial stromal cells used in embryo co-culture systems

Abstract

Purpose Along with comparative investigation of the decidualization potential and IL-6 secretion by fresh and frozen ESCs, we also aimed to evaluate the effectiveness of co-culture systems based on fresh or frozen ESCs in terms of clinical pregnancy rates. Methods Outcome analysis of a total of 215 IVF cycles with co-culture with fresh or frozen ESCs was performed. Endometrial tissue was obtained from 17 healthy donors. Concentrations of secreted prolactin, IGFBP-1, and IL-6 in conditioned media from cultured fresh and frozen ESCs (decidualized or not) were measured using ELISA or ECLIA. Results Embryo co-culture with frozen ESCs resulted in a much lower pregnancy rate compared to the alternative system using fresh ESCs. Furthermore, cultivated frozen ESCs showed considerably decreased release of prolactin, IGFBP-1, and IL-6 compared to fresh ESCs, indicating that cryopreservation negatively affects their decidualization potential and cytokine production.Conclusions Altogether, this data illustrates the need for optimization and improvement of the existing autologous endometrial co-culture systems.

Increasing of malignancy of breast cancer cells after cryopreservation: molecular detection and activation of angiogenesis after CAM-xenotransplantation

“…As reported, cryopreservation adversely affected the decidualization potential and cytokine production of human endometrial stromal cells [4]. The activity of xenobiotic metabolizing enzymes and responsiveness to enzyme-inducing agents reduced in cryopreserved human hepatocytes compared with that in freshly isolated cells [5].…”

Autologous endometrial cell co-culture improves human embryo development to high-quality blastocysts: a randomized controlled trial

“…The establishment of autocrine and paracrine communications and cell-tocell interactions between the endometrial feeder cells and the embryo should contribute to the detoxification of the culture medium, facilitating blastocyst development and improving the implantation rate (Bochev et al, 2016;De los Santos et al, 1996;Guérin et al, 2001;Mercader et al, 2006;Simón et al, 1999). Many teams concentrating their studies more specifically on patients with IVF failure such as poor ovarian reserve, history of poor-quality embryos or repeated implantation failure, demonstrated the benefits of AECC in in-vitro treatments (Eyheremendy et al, 2010;Spandorfer et al, 2002aSpandorfer et al, ,2004.…”