Effect of Cryopreserved Placental Explants on Female Reproductive System Under Normal and Pathological Conditions (Experimental study)

Prokopіuk, Volodymyr; Grischenko, Olga V.; Prokopyuk, Oleksandra; Shevchenko, Nadiia; Falko, Oksana V.; Сторчак, А. В.; Schedrov, Andrii O.

doi:10.15407/cryo27.03.250

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…The effectiveness of MIBP used in obstetricgynecological pathology is explained by focusing the placenta biological effect to the processes of pregnancy and the female body (through the synthesis of primarily chorionic gonadotropin, alphafetoprotein and progesterone [2]), and the specifics of the institutions that used the products. The results obtained are consistent with research data on the biological effects of placental derivatives on experimental obstetric-gynecological pathology in both in vitro and in vivo models [7,[10][11][12].…”

Section: Resultssupporting

confidence: 89%

“…Thus, during pregnancy, after MIBP implantation, the functioning of the reproductive system changes first. The results obtained are consistent with research data, which also indicate the effectiveness of placenta and stem cell conditioned media, most notably in hormonal, dystrophic and autoimmune disorders of the reproductive system [7,10,11].…”

Section: Discussionsupporting

confidence: 90%

“…This has led to an increased interest in regenerative medicine, stem cells and their derivatives. The publications describe both clinical and experimental use of placenta derivatives in various fields of medicine: in therapy, surgery, endocrinology, obstetrics and gynecology, ophthalmology, neurology, dermatology, urology, orthopedics, traumatology and others [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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Experience in Clinical Application of Cryopreserved Placental Derivatives: Cells, Tissue, Membranes, Extract, and Cord Blood Serum

Prokopіuk

Karpenko

Shevchenko

et al. 2020

Innov Biosyst Bioeng

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Background. The human placenta is a promising source of biomaterial for regenerative medicine. This is primarily due to the availability of a sufficient amount of material, low immunogenicity, a large number of stem cells, and high proliferative cell potential. The effectiveness of stem cells, their lysates, and conditioned media in various pathological conditions has been proven in many studies. Most studies are experimental, or are in different phases of clinical trials. At the same time, in Ukraine there is a wealth of experience of clinical using the cryopreserved placenta medico-immunobiological preparations, which can be the basis for further application of placental material. Objective. We aim to perform a critical analysis of data on the effectiveness and prospects of application of the cryopreserved placenta preparations (cells, tissues, membranes, extract and cord blood serum) in clinical practice. Methods. The results of clinical application of 2,579 medical immunobiological Platex and Cryocell placenta preparations were analyzed. An attention was paid to the effectiveness, course of concomitant, comorbid pathology in patients, as well as the presence of negative responses and complications. The number of the preparations used and the one of the patients according to the nosological forms were counted. Results. The experience of application of medical immunobiological preparations of placental origin in obstetric-gynecological, therapeutic, neurological and endocrinological pathologies is analyzed in the work. The obtained data are compared with the research results in the corresponding model experiments of in vitro and in vivo systems. The positive effect of placental preparations on the course of miscarriage, climacteric syndrome, infertility, diabetes mellitus, coronary heart disease, multiple sclerosis, amyotrophic lateral sclerosis, trophic ulcers has been determined. Contraindications for placenta preparations are some malignancies and an infectious disease process without proper pathogen elimination. Conclusions. Cryopreserved placenta preparations are effective when used in obstetric, gynecological, neurological, endocrinological and therapeutic practice. Their effect is primarily observed in diseases, which are accompanied by autoimmune reactions, hormonal disorders, dysplastic or degenerative processes. The restricted application of placenta preparations is infectious disease process without proper pathogen elimination.

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Section: Resultssupporting

confidence: 89%

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

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Experience in Clinical Application of Cryopreserved Placental Derivatives: Cells, Tissue, Membranes, Extract, and Cord Blood Serum

Prokopіuk

Karpenko

Shevchenko

et al. 2020

Innov Biosyst Bioeng

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Influence of Cryopreserved and Freshly Isolated Placental Explants and Cells - Conditioned Media on Murine Uterine and Ovarian Organotypic Cultures

Prokopіuk

2018

Probl Cryobiol Cryomed

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Вплив середовищ, кондиційованих кріоконсервованими та свіжовиділеними експлантами та клітинами плаценти, на органотипові культури маток та яєчників мишейРеферат: У сучасній репродуктології широко застосовуються методи клітинної та тканинної терапії, перспективними обʼєктами якої є похідні плаценти, що впливають безпосередньо на жіночу репродуктивну систему та ланки її регуляції. Механізми такої взаємодії залишаються остаточно нез'ясованими. У роботі досліджено вплив середовищ, кондиційованих кріоконсервованими і нативними клітиними та експлантами плаценти, на органотипові культури яєчників, маток мишей та культуру фібробластів. Культури оцінено за допомогою морфологічних методів та метаболічних тестів. Показано, що кондиційовані середовища стимулюють метаболічну активність культур маток та пригнічують метаболічну активність культур яєчників. Відсутність реакції фібробластів на ці середовища свідчить про вплив гуморальних факторів плацентарного походження безпосередньо на генеративні елементи яєчника та компоненти ендометрію без залучення елементів сполучної тканини. Встановлено, що властивості середовищ, кондиційованих свіжовиділеними та кріоконсервованими клітинами та експлантатами плаценти, значуще не відрізнялися.Ключові слова: плацента, мезенхімальні стовбурові клітини, яєчник, матка, фібробласти, культивування.Реферат: В современной репродуктологии широко используются методы клеточной и тканевой терапии, перспективными объектами которой являются производные плаценты, которые влияют непосредственно на женскую половую систему и уровни ее регуляции. Механизмы такого взаимодействия остаются окончательно невыясненными. В работе изучено влияние сред, кондиционированных криоконсервированными, свежевыделенными клетками и эксплантами плаценты, на изолированные культуры яичников, маток мышей и культуру фибробластов. Культуры оценены с помощью морфологических методов и метаболических тестов. Показано, что кондиционированные среды стимулируют метаболическую активность культур маток и угнетают метаболическую активность культур яичников. Отсутствие реакции фибробластов на эти среды свидетельствует о влиянии гуморальных факторов плацентарного происхождения непосредственно на генеративные элементы яичников и эндометрий без задействования элементов соединительной ткани. Установлено, что свойства сред, кондиционированных нативными, криоконсервированными клетками и эксплантами плаценты, значимо не отличались.Ключые слова: плацента, мезенхимальные стволовые клетки, яичник, матка, фибробласты, культивирование. Abstract: Contemporary reproductive medicine involves the methods of cell and tissue therapy, which among others involve the placental products, affecting mainly female reproductive system and its regulation levels. The mechanisms of this interaction have still remained unclear. Here the influence of the media, conditioned with cryopreserved and fresh placental cells and explants on isolated murine ovarian, uterine organotypic cultures and fibroblast culture was studied. The cultures were evaluated using morphological meth...

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Effect of Cryopreserved Placental Explants on the Ovary Restoration After Torsion Treatment

Prokopіuk

Loginova

Prokopiuk

et al. 2018

WOMAB

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Effect of Cryopreserved Placental Explants on Female Reproductive System Under Normal and Pathological Conditions (Experimental study)

Cited by 6 publications

References 18 publications

Experience in Clinical Application of Cryopreserved Placental Derivatives: Cells, Tissue, Membranes, Extract, and Cord Blood Serum

Experience in Clinical Application of Cryopreserved Placental Derivatives: Cells, Tissue, Membranes, Extract, and Cord Blood Serum

Influence of Cryopreserved and Freshly Isolated Placental Explants and Cells - Conditioned Media on Murine Uterine and Ovarian Organotypic Cultures

Effect of Cryopreserved Placental Explants on the Ovary Restoration After Torsion Treatment

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