Effect of Cultivar on the Protection of Cardiomyocytes from Oxidative Stress by Essential Oils and Aqueous Extracts of Basil (Ocimum basilicum L.)

Danesi, Francesca; Elementi, Simona; Neri, Roberta; Maranesi, Magda; D’Antuono, Luigi Filippo; Bordoni, Alessandra

doi:10.1021/jf8018547

Cited by 39 publications

(30 citation statements)

References 30 publications

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“…The genus Ocimum L. includes approximately 150 species, possessing a great variation in plant morphology and biology, essential oil content, and chemical composition (Danesi et al, 2008). Ocimum basilicum, popular known as Basil or Sweet Basil, is a common herb that belongs to Lamiaceae family.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of basil extract ( Ocimum basilicum L.) on oxidative, anti-genotoxic and anti-inflammatory effects in human leukocytes cell cultures exposed to challenging agents

Güez

Souza

Fischer

et al. 2017

Braz. J. Pharm. Sci.

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Ocimum is one of the most important genera of the Lamiaceae family. Several studies about basil and its popular use reveal many characteristics of the herb, including its use as antioxidant, anti-aging, antiinflammatory, anti-carcinogenic, anti-microbial, and cardiovascular agents, among others. In this paper, we evaluated genotoxic, oxidative, and anti-inflammatory parameters from the extract of Ocimum basilicum in different concentrations, using human leukocytes cultures exposed to challenging agents. Our results confirm that the O. basilicum extract acts as an antioxidant and effectively reverts or subjugates the effects of high oxidizing agents such as hydrogen peroxide. These actions are attributed to its composition, which is rich in polyphenols and flavonoids as well as compounds such as rosmarinic acid, all of which have well-known antioxidant activity. We also show that our basil extract presents anti-inflammatory properties, the mechanism of which is a composed interaction between the inhibition of pro-inflammatory mediator and the stimulation of anti-inflammatory cytokines. Although pharmacodynamics studies are necessary to evaluate the activities in vivo, our results demonstrated that basil could act as an antioxidant and anti-inflammatory and a possible alternative for medicinal treatment. Uniterms:Ocimum basilicum/evaluation. Ocimum basilicum/genotoxic effects. Ocimum basilicum/ anti-inflammatory properties. Medicinal plants/study.

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Section: Introductionmentioning

confidence: 99%

Evaluation of basil extract ( Ocimum basilicum L.) on oxidative, anti-genotoxic and anti-inflammatory effects in human leukocytes cell cultures exposed to challenging agents

Güez

Souza

Fischer

et al. 2017

Braz. J. Pharm. Sci.

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“…It is well-known that some plants, especially those belonging to the Lamiaceae family, possess a wide range of biological and pharmacological activities (Danesi et al, 2008). O. basilicum has been cultivated from ancient times both as an ornamental plant and as a major essential oil crop.…”

Section: Discussionmentioning

confidence: 99%

Pollination following grafting introduces efficiently Ocimum basilicum L. genes into Nicotiana tabacum L.

Wei

Yang

Wei

2013

Span. j. agric. res.

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Tobacco is an important cash crop in the world. However, the genetic basis is comparatively narrow among the modern Nicotiana tabacum cultivars, limiting its potential for quality improvement. To introduce genes conferring desirable chemical constituents from medicinal plants, a distant hybridization test was conducted between N. tabacum and Ocimum basilicum L. Seedlings of wild type Nicotiana sylvestris and N. tabacum cultivar 78-04 respectively acted as rootstock and scion. During the flowering season, hand pollination between 78-04 as pistillate parent and O. basilicum as pollen parent was carried out under 22-25°C temperature and 70-80% of relative humidity in the greenhouse. Seed sets of 55% were obtained in 78-04, and about 400 seeds per capsule were produced. But both non-grafted and self-grafted 78-04 plants rarely resulted in fruits by hand pollination and those obtained were without seed. Similar results were obtained in different material combination. The interfamilial F1 hybrids acquired showed distinct variation with various morphological characteristics, and their hybrid nature was confirmed by isozyme and random amplified polymorphic DNA (RAPD) analyses. This result indicated that pollination following grafting can facilitate gene exchange and recombination at the interfamilial level and efficiently overcome barriers of sexual incompatibility between N. tabacum and O. basilicum. Our research not only extends the genetic basis of tobacco but also will provide valuable germplasm for improvement of varieties.

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“…The results were expressed as micrograms of gallic acid equivalents (GAE) per mg of extract. Total antioxidant capacity (TAC) was evaluated using the method of Re et al [17], as previously described [18]. Briefly, TAC was measured as the quenching of the absorbance at 734 nm (DU730 spectrophotometer; Beckman Coulter Inc., CA, USA).…”

Section: Analysis Of Plant Extractsmentioning

confidence: 99%

“…The investigation conforms to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication 85-23, revised 1996). Cells were isolated and cultured as previously described [18]. At seeding, the culture medium was replaced with a fresh one containing two different concentrations of kale extract solution (10 or 20 μL•mL −1 medium), or 20 μM TC.…”

Section: Cell Culturementioning

confidence: 99%

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The Agronomic Techniques as Determinants of the Phenolic Content and the Biological Antioxidant Effect of Palm-Tree Kale

Danesi¹,

Valli²,

Elementi³

et al. 2014

FNS

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The content of phytochemicals in vegetables is strongly affected by genetic, agronomic, and environmental factors, and by transportation and storage conditions, potentially affecting the sensory and putative health-promoting properties. In this study the impact of different agricultural techniques on the phenolics content and antioxidant activity of extracts from a traditional Italian kale landrace, called palm-tree kale (Brassica oleracea L., ssp. acephala DC, var. sabellica L.) was evaluated. Furthermore, the antioxidant effects of the different extracts were assessed in a biological system using primary cultures of neonatal rat cardiomyocytes. The final aim was to evidence whether and how the agronomic practices can affect the antioxidant properties of kale not only in vitro but also in cells. Results herein reported underline the influence of the crop production strategies in establishing the potential health benefits of a vegetable. This research approach could be useful for the selection of production techniques that are able to provide edible vegetables with higher functional activity, and evidences the need to study the food domain as a whole with the nutrition domain, and to integrate all results in order to have an overall "foodomics" vision allowing the improvement of health and well-being.

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Effect of Cultivar on the Protection of Cardiomyocytes from Oxidative Stress by Essential Oils and Aqueous Extracts of Basil (Ocimum basilicum L.)

Cited by 39 publications

References 30 publications

Evaluation of basil extract ( Ocimum basilicum L.) on oxidative, anti-genotoxic and anti-inflammatory effects in human leukocytes cell cultures exposed to challenging agents

Evaluation of basil extract ( Ocimum basilicum L.) on oxidative, anti-genotoxic and anti-inflammatory effects in human leukocytes cell cultures exposed to challenging agents

Pollination following grafting introduces efficiently Ocimum basilicum L. genes into Nicotiana tabacum L.

The Agronomic Techniques as Determinants of the Phenolic Content and the Biological Antioxidant Effect of Palm-Tree Kale

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