Effect of culture conditions and induction strategies on production of human interleukin-6 by a recombinant Aspergillus nidulans strain

Yadwad, V. B.; Wilson, S.; Ward, Owen P.

doi:10.1016/s0953-7562(96)80169-9

Cited by 17 publications

(7 citation statements)

References 26 publications

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“…The exhaustion of glucose in the medium seems to be the trigger for production of proteases. The results are consistent with the report that human interleukin-6 secreted by recombinant Aspergillus nidulans was degraded by extracellular proteases after the cell concentration reached its maximum at 48 h of fermentation [23].…”

Section: Protease Activity In Ph Controlled Fermentationssupporting

confidence: 92%

Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity

O’Donnell

Wang

et al. 2001

Biochemical Engineering Journal

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Section: Protease Activity In Ph Controlled Fermentationssupporting

confidence: 92%

Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity

O’Donnell

Wang

et al. 2001

Biochemical Engineering Journal

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“…Years later, Yadwad and colleagues (1996) 51 produced approximately 54 mg/L of interleukin-6 in an air-lift fermenter with a recombinant strain of A. nidulans and a medium supplemented with salts, fructose, and threonine.…”

Section: Upstream Processing On Biopharmaceuticals Productionmentioning

confidence: 99%

Biopharmaceuticals from microorganisms: from production to purification

Jozala

Geraldes

Tundisi

et al. 2016

Brazilian Journal of Microbiology

154

105

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The use of biopharmaceuticals dates from the 19th century and within 5–10 years, up to 50% of all drugs in development will be biopharmaceuticals. In the 1980s, the biopharmaceutical industry experienced a significant growth in the production and approval of recombinant proteins such as interferons (IFN α, β, and γ) and growth hormones. The production of biopharmaceuticals, known as bioprocess, involves a wide range of techniques. In this review, we discuss the technology involved in the bioprocess and describe the available strategies and main advances in microbial fermentation and purification process to obtain biopharmaceuticals.

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“…This suggests that most of this protease activity is released from the mycelium as a result of cell lysis. After maximum biomass and recombinant hIL-6 activity were observed in a recombinant A nidulans fermentation, Yadwad et al (1996) observed parallel dramatic reductions in biomass and hIL-6 activity, which were attributed to mycelial shearing and protease release. Less protease activity was found in fungal culture supernatants where the cell morphology was filamentous, whereas high protease activity was associated with supernatants where mycelia had a pelleted morphology.…”

Section: Discussionmentioning

confidence: 88%

Culture-Based Strategies for Reduction of Protease Activity in Filtrates from Aspergillus niger NRRL-3

Ahamed

Singh

Ward

2005

World J Microbiol Biotechnol

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While Aspergillus strains are also being considered as potential hosts for production of extracellular heterologous proteins, the proteases produced by the host are highly problematic in that they typically modify and degrade the recombinant proteins. Culture-based approaches for minimization of protease activity in culture supernatants of Aspergillus niger NRRL-3 included reduction or elimination of peptide nitrogen in the medium, preferential use of a defined salts medium rather than a non-peptide nitrogen medium containing yeast-nitrogen base, supplementation of the medium with carboxymethylcellulose and cultivation at pH 6.5 rather than 7.5. In general, increased proteolytic activity was observed after maximum biomass was observed and biomass was declining suggesting the majority of protease activity was released by cell lysis. Carboxymethylcellulose shifted mycelial morphology from pelleted to filamentous. Mycelium lysis in the centre of pellets, with resultant release of intracellular proteases, would explain why filamentous cultures exhibited much lower proteolytic activity than pelleted cultures.

show abstract

Effect of culture conditions and induction strategies on production of human interleukin-6 by a recombinant Aspergillus nidulans strain

Cited by 17 publications

References 26 publications

Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity

Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity

Biopharmaceuticals from microorganisms: from production to purification

Culture-Based Strategies for Reduction of Protease Activity in Filtrates from Aspergillus niger NRRL-3

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