Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos

Lonergan, P.; Fair, Trudee; Corcoran, D; Evans, A.C.O.

doi:10.1016/j.theriogenology.2005.09.028

Cited by 209 publications

(143 citation statements)

References 84 publications

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“…However, the drawbacks to using serum include variability in composition between batches, its undefined nature and the risk of contamination. In addition, the presence of serum during culture alters gene expression and tolerance to cryopreservation, when compared to in vivo embryos [for review see Lonergan et al 2006). Similar results are found in mice (Khosla et al, 2001).…”

Section: Introductionmentioning

confidence: 88%

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

et al. 2015

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In vitro production (IVP) of bovine embryos is not only of great economic importance to the cattle industry, but is also an important model for studying embryo development. The aim of this study was to evaluate the histone modification, H3R26me2 during pre-implantation development of IVP bovine embryos cultured with or without serum supplementation and how these in vitro treatments compared to in vivo embryos at the morula stage. After in vitro maturation and fertilization, bovine embryos were cultured with either 0 or 2.5% fetal bovine serum (FBS). Development was evaluated and embryos were collected and fixed at different stages during development (2-, 4-, 8-, 16-cell, morula and blastocyst). Fixed embryos were then used for immunofluorescence utilizing an antibody for H3R26me2. Images of stained embryos were analyzed as a percentage of total DNA. Embryos cultured with 2.5% FBS developed to blastocysts at a greater rate than 0%FBS groups (34.85±5.43% vs. 23.38±2.93%; P<0.05). Levels of H3R26me2 changed for both groups over development. In the 0%FBS group, the greatest amount of H3R26me2 staining was at the 4-cell (P<0.05), 16-cell (P<0.05) and morula (P<0.05) stages. In the 2.5%FBS group, only 4-cell stage embryos were significantly higher than all other stages (P<0.01). Morula stage in vivo embryos had similar levels as the 0%FBS group, and both were significantly higher than the 2.5%FBS group. These results suggest that the histone modification H3R26me2 is regulated during development of pre-implantation bovine embryos, and that culture conditions greatly alter this regulation.

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Section: Introductionmentioning

confidence: 88%

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

et al. 2015

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“…Warming and survival cultures were made in three batches. Different superscripts within columns differ significantly ( a,b P < 0.03; c,d,e P < 0.05; f ,g,h P < 0.02) 6.0 ± 6.9 e 5.9 ± 7.1 h a review, see Lonergan et al 2006;Lonergan 2007;Lonergan and Fair 2008). In the present study, ATRA increased the development of Day 8 blastocysts compared with LG treatment and control, although significant differences between ATRA and LG treatment groups were already evident on Day 7.…”

Section: Discussionmentioning

confidence: 99%

127 Development and Quality of Bovine Morulae Cultured in Serum-Free Medium With Retinoic Receptor Specific Agonists

Gómez¹,

Caamaño²,

Muñoz³

et al. 2008

Reprod. Fertil. Dev.

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Abstract.Retinoids regulate development and differentiation of the bovine blastocyst in vitro, although the underlying mechanisms remain to be clarified. A challenge in reproductive biotechnology is the identification of pathways that regulate early embryonic development and their influence on blastocyst differentiation, apoptosis and survival to cryopreservation as traits of embryo quality. The present paper analyses the effects of short-term exposure (24 h) to retinoids on in vitroproduced bovine morulae. Immature cumulus oocyte complexes were in vitro matured and fertilised. Presumptive zygotes were subsequently cultured in modified synthetic oviduct fluid up to Day 6, in which morulae were randomly allocated to the different experimental groups. The treatments consisted of 0.1 µm LG100268 (LG; a retinoid X receptor agonist), 0.7 µm all-trans retinoic acid (ATRA; a retinoic acid receptor agonist) or no additives. Day 8 blastocyst development was increased in the ATRA-treated group compared with the LG and untreated embryos. In Day 7 embryos, the number of total cells and cells allocated to the trophectoderm were higher in the ATRA-treated group compared with untreated embryos. Apoptosis in the inner cell mass increased after LG treatment, whereas ATRA had no effect. After vitrification and warming, survival and hatching rates of Day 7 blastocysts did not change with retinoid treatment. Within the LGtreated and untreated blastocyst groups, survival and hatching rates were higher for Day 7 than Day 8 embryos; however, Day 8 blastocysts treated with ATRA showed improved hatching rates. In conclusion, treatment of morulae with ATRA in serum-free medium improves embryo development and quality without increasing the incidence of apoptosis and necrosis.

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“…The development potential of embryos obtained in vitro is lower in comparison with embryos obtained in vivo due to suboptimal conditions during oocyte maturation, their subsequent fertilization, and embryonic development (Wright and Ellington, 1995;Young et al, 1998;Lonergan et al, 2006;Metwally and Ledger, 2011).…”

Section: Development Potential Of Embryos Obtained In Vitromentioning

confidence: 99%

Obtaining farm animal embryos <i>in vitro</i>

Duszewska¹,

Rapala²,

Trzeciak³

et al. 2012

J. Anim. Feed Sci.

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This paper reviews the basic knowledge about obtaining farm animal embryos in vitro with special focus on differences among species and application of this procedure in the future. In vitro production of farm animal embryos consists of in vitro maturation (IVM) of oocytes, in vitro fertilization (IVF) of matured oocytes, and in vitro culture (IVC) of embryos. Oocytes can be collected from live animals (by laparotomy, laparoscopy, Ovum Pick Up) or from slaughtered ones (by puncture, sectioning). Usually immature oocytes are isolated, and during IVM they reach maturity. Matured oocytes are cultured with sperm (IVF), leading to the formation of zygotes. In the case of fertilization problems (horse, pig), intracytoplasmic sperm injection is used. The zygotes are usually cultured (IVC) to the morula and blastocyst stages. These embryos can be transferred to recipients or frozen/vitrified. Offspring have been obtained after transfer of cattle, sheep, goat, pig and horse embryos. This procedure can be used in animal breeding, biotechnology, medicine, and basic research.

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Effect of culture environment on gene expression and developmental characteristics in IVF-derived embryos

Cited by 209 publications

References 84 publications

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

Supplementation of fetal bovine serum alters histone modification H3R26me2 during preimplantation development of in vitro produced bovine embryos

127 Development and Quality of Bovine Morulae Cultured in Serum-Free Medium With Retinoic Receptor Specific Agonists

Obtaining farm animal embryos <i>in vitro</i>

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