Effect of Culture Media and Growth Hormones on Callus Induction in Aquilaria 
 malaccensis Lam., a Medicinally and Commercially Important Tree Species 
 of North East India

Saikia, Moitreyee; Shrivastava, Karuna; Singh, S. S.

doi:10.3923/ajbs.2013.96.105

Cited by 11 publications

(5 citation statements)

References 4 publications

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“…Das et al (2015 revealed that callus was initiated and developed in B5 medium supplemented with hormonal combination of 2,4-D and kinetin, whereas other combinations using GA3, indole-3-acetic acid, IBA, and 6-Benzylaminopurine in B5 medium resulted in no callus initiation even after 80 days. Additionally, Dalila et al (2013) recorded callus induction of >60%; and Saikia et al (2013) recorded 70%-73% callus induction using the combination of 2,4-D and kinetin supporting our findings. To sum up, the hormonal combination of 2,4-D and kinetin in Gamborg's B5 medium is recommended to generate fine quality callus mass along with high production percentage.…”

Section: Callus Inductionsupporting

confidence: 90%

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Gauchan¹,

Bhuju²,

Lamichhane³

et al. 2020

J Appl Pharm Sci

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Taxus wallichiana, an endangered Taxus species native to Nepal, is a source of Taxol (paclitaxel), an anticancer drug, which if produced via plant cell culture in vitro not only reduces the pressure on natural habitat but also allows continuous production throughout the seasons. Gamborg's B5 medium was the culture medium to develop callus and suspension culture. Quantification of Taxol was carried out via high performance liquid chromatography (HPLC) using C18 column, water-acetonitrile mobile phase, and 1 ml/minute flowrate at 227 nm wavelength. Genetic variation among population was determined using the POPGEN32, version 1.31. Best callus response was in medium with 1 mg/l 2,4-D for both stem and needle explants. For cell suspension culture, callus from needle explants showed better response compared to stem. Fructose and sucrose proved to be the best carbon sources. Cell count increased as carbon concentration increased. 2,4-D at low concentration (<2 mg/l) and naphthaleneacetic acid at high concentration (>5 mg/l) showed higher cell growth rate. Inoculum volume was directly proportional to cell count. Extra addition of macronutrients had inhibitory effect on cell count, but the effect was reversed for MgSO 4 . Using HPLC, for bark, the highest Taxol yield was 0.057 mg/g distilled water (DW), while for needle was 0.056 mg/g DW. For suspension cultured HPLC samples, Taxol yield ranged at 0.004%. Size range of amplified Deoxyribonucleic acids was between 200 and 2,000 bp. Thus, this investigation generated a concrete data for Taxus population of Nepal providing efficient protocols for establishment of callus and cell suspension culture with the aim of producing Taxol in in vitro conditions.

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Section: Callus Inductionsupporting

confidence: 90%

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Gauchan¹,

Bhuju²,

Lamichhane³

et al. 2020

J Appl Pharm Sci

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“…The present findings revealed that the supplementation of BA at an optimum concentration with 2,4-D is required to produce calli or cell culture with viable cell morphology. Meanwhile, Saikia et al [31] reported that the hormonal combination of 2,4-D and kinetin was found to be effective in producing optimum callus induction in Aquilaria malaccensis i.e. 70-73 %.…”

Section: Resultsmentioning

confidence: 99%

Purification, Fractionation and Characterization of Anthocyanin from in vitro Culture of Bridelia retusa (L.) Spreng.

Aswathy¹,

Saied²,

Lawarence³

et al. 2018

pharmaceutical-sciences

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Anthocyanins are water-soluble secondary metabolites, belonging to flavonoids, polyphenolic molecules containing 15 C chains with an aromatic ring, and one or more sugar groups attached at diverse positions to form hydroxylated basic structure. They occur mainly as glycosides of anthocyanidins. Six anthocyanidins are common in leaves, stems, roots, flowers and fruit such as pelargonidin, cyanidin, peonidin, delphinidin, petunidin and malvidin. Due to dietary antioxidant properties, many works have been attempted to analyse the properties of anthocyanins extracts from many plant species.In vitro cell cultures are capable of synthesizing and accumulating diverse phytochemicals with medicinal or nutritional value. Alkaloids, saponins, anthraquinones, polyphenols and terpenes have been synthesized via in vitro culture. Among them anthocyanins have significance as natural dye and as antioxidant [1] . Characterization of promoter sequences, regulatory genes and transcription factors involved in anthocyanin synthesis facilitates the in vitro synthesis effectively [2] . Genetic engineering of anthocyanin

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“…Salah satu teknik dalam kultur in vitro yang sering digunakan untuk mikropropagasi adalah induksi kalus yang mampu menghasilkan sel-sel yang bersifat meristematik (Efferth, 2019). Adapun protokol yang secara efisien mampu menginduksi kalus dengan zat pengatur tumbuh (ZPT) pada spesies Gyrinops walla dan Aquilaria malaccensis telah dilaporkan oleh Munasinghe et al, (2017) dan Saikia et al, (2013) dengan menggunakan eksplan daun. Hanya saja, pertumbuhan dan perkembangan kalus yang diperoleh dilaporkan masih sangat lambat.…”

Section: Pendahuluanunclassified

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

Marisa

Hidayati

Sasongko

et al. 2021

JBT

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Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This plant has been threatened by overexploitation which leads on decreasing in the natural population. This research aimed to induce in vitro callus formation to support the sustainable utilization of G. versteegii, which in the end, may support plant productivity. Callus induction was investigated using cotyledon and cultured on MS medium supplemented with several combination of plant growth regulators. The observations were done for the number of explants turned into callus and the duration for the first callus formation. The results showed that combination of 3 mg/L NAA + 0.5 mg/L BAP was recorded as the best combination for callus induction (63.63%). Callus with friable structure and bright color are obtained within nine days of incubation and showed the characteristic of embryonic callus. This result is expected to give a significant opportunity to conserve the natural population of G. versteegii.

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Effect of Culture Media and Growth Hormones on Callus Induction in Aquilaria malaccensis Lam., a Medicinally and Commercially Important Tree Species of North East India

Cited by 11 publications

References 4 publications

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Establishment of regenerative callus, cell suspension system, and molecular characterization of Taxus wallichiana Zucc. for the in vitro production of Taxol

Purification, Fractionation and Characterization of Anthocyanin from in vitro Culture of Bridelia retusa (L.) Spreng.

Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke

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