Effect of culture media on antheridial configuration in Phytophthora boehmeriae

Abstract

The position of the antheridium on the oogonium of Phytophthora boehmeriae was greatly affected by culture media. Agar medium containing lecithin and basal salts and corn grain agar favored the production of paragynous antheridia, while corn meal agar, lima bean agar, vegetable juice agar, and tomato agar were conducive to formation of amphigynous antheridia. The effect of culture media on antheridial configuration was found to be due to nutrient concentration. A high concentration of nutrients in the media was favored the formation of amphigynous antheridia, and a low concentration of nutrients favored paragynous antheridia.Key words: antheridial configuration, oogonium, nutrients, Phytophthora boehmeriae.

Phenotypic variation in a clonal lineage of two Phytophthora cinnamomi populations from Western Australia

“…Variation in these characters was low compared to paragyny proportions or growth on PDA. However, as commented earlier, comparisons among studies are difficult as nutrition affects oospore and oogonia size (Chang, Shepherd & Pratt 1974, Zentmyer, Klure & Pond 1979 and paragyny proportion (Gao et al 1998). These examples emphasize that comparison of organ measurements needs to be approached with caution unless the methods used are well defined.…”

Phylogenetic relationships of Phytophthora species based on ribosomal ITS I DNA sequence analysis with emphasis on Waterhouse groups V and VI

“…In the study by Hu$ berli, Tommerup & St Hardy (1997) paragynous antheridia were found to be widespread among Australian and Papua New Guinean isolates of the heterothallic species P. cinnamomi. In addition, in P. boehmeriae, which was originally described as having only amphigynous antheridia, paragynous antheridia were produced predominantly on certain culture media (Gao et al 1998).…”

Phosphite impact on the in vitro production and viability of selfed oospores by Phytophthora cinnamomi

“…1990). It has been shown for Phytophthora boehmeriae that medium nutrient conditions influence whether amphigynous or paragynous antheridia will predominate (G ao et al. 1998).…”

“…(2003) showed the presence of both paragynous and amphigynous antheridia in A1 × A2 mating type pairings of P. cinnamomi , although proportions of oospores with paragynous antheridia were mostly low (0.2–10%). This may have been due to the use of a high‐nutrient medium which has been found to favour amphigynous antheridia in P. boehmeriae (G ao et al. 1998).…”