Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos

Oh, Hyun Ju; Lee, Tae Hee; Lee, Ji Hyun; Oh, Seoung Wug; Lee, Ju Hyun; Kim, Jin Wook; Kim, Se Woon; Lee, Byeong Chun

doi:10.1016/j.theriogenology.2013.09.018

Cited by 9 publications

(5 citation statements)

References 43 publications

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“…A total of 120 oocytes matured in vivo were recovered from female donors by flushing their oviducts, and the ASCs developed from Snuppy were injected into the perivitelline space of 112 enucleated oocytes, of which 97 couplets were fused and activated. In keeping with our previous report that dog ASCs cultured with Dulbecco’s Modified Eagle Medium (DMEM) increased the fusion rate of dog to cow interspecies SCNT 18 , ASCs cultured with DMEM produced an improved mean fusion rate of 86.6% (range 75.0% –100.0%) from our previously achieved mean rate of 67.2% (range 46.7–85.0%, unpublished data) in dog to dog SCNT. A total 94 reconstructed SCNT embryos were transferred to the oviducts of seven naturally synchronized recipient dogs.…”

Section: Resultssupporting

confidence: 88%

Birth of clones of the world’s first cloned dog

Kim

Setyawan

et al. 2017

Sci Rep

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Animal cloning has gained popularity as a method to produce genetically identical animals or superior animals for research or industrial uses. However, the long-standing question of whether a cloned animal undergoes an accelerated aging process is yet to be answered. As a step towards answering this question, we compared longevity and health of Snuppy, the world’s first cloned dog, and its somatic cell donor, Tai, a male Afghan hound. Briefly, both Snuppy and Tai were generally healthy until both developed cancer to which they succumbed at the ages of 10 and 12 years, respectively. The longevity of both the donor and the cloned dog was close to the median lifespan of Afghan hounds which is reported to be 11.9 years. Here, we report creation of 4 clones using adipose-derived mesenchymal stem cells from Snuppy as donor cells. Clinical and molecular follow-up of these reclones over their lives will provide us with a unique opportunity to study the health and longevity of cloned animals compared with their cell donors.

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Section: Resultssupporting

confidence: 88%

Birth of clones of the world’s first cloned dog

Kim

Setyawan

et al. 2017

Sci Rep

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“…It seems the methylation status of donor cells tend to be remained in SCNT embryos even after several cell divisions in embryos. In support of this, in blastocysts derived from treated MSCs in which the DNMT1 expression was downregulated, DNMT1 expression was lower than cloned blastocysts derived from untreated MSCs (Kim et al, 2014). Therefore, in cattle cloned embryos there is a tendency to preserve the DNA methylation patterns inherited from their donor cells (Bourc'his et al, 2001).…”

Section: Igf2 Igf2rmentioning

confidence: 86%

The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes

Nazari¹,

Shirazi²,

Shams‐Esfandabadi³

et al. 2016

Int. J. Dev. Biol.

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Nuclear reprogramming of a differentiated cell in somatic cell nuclear transfer (SCNT) is a major concern in cloning procedures. Indeed, the nucleus of the donor cell often fails to express the genes which are a prerequisite for normal early embryo development. This study was aimed to evaluate the developmental competence and the expression pattern of some reprogramming related genes in bovine cloned embryos reconstructed with amniotic membrane stem cells (AMSCs) in comparison with those reconstructed with mesenchymal stem cells (MSCs) and adult fibroblasts (AF) as well as with in vitro fertilized (IVF) oocytes. In vitro matured abattoir-derived oocytes were considered as recipients and a hand-made cloning technique was employed for oocyte enucleation and nuclear transfer (NT) procedures. The expression pattern of genes involved in self-renewal and pluripotency (POU5F1, SOX2, NANOG), imprinting (IGF2, IGF2R), DNA methylation (DNMT1, DNMT3A), histone deacetylation (HDAC2), and apoptosis (BAX, BCL2) were evaluated in NT and IVF derived embryos. Despite the insignificant difference in cleavage rate between reconstructed and IVF oocytes, the blastocyst rate in the IVF group was higher than that of other groups. Among reconstructed oocytes, a higher blastocysts rate was observed in MSC-NT and AMSCs-NT derived embryos that were significantly higher than AF-NT derived ones. There were more similarities in the expression pattern of pluripotency and epigenetic modification genes between MSC-NT and IVF derived blastocysts compared with other groups. In conclusion, considering developmental competence, AMSCs, as alternative donors in SCNT procedure, like MSCs, were prone to have more advantage compared with AF.

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“…In order to separate cumulus cells and immature oocytes, the COCs were denuded by vortexing in 0.1% (w/v) hyaluronidase. After vortexing, the denuded oocytes were stored at −80°C before use for RNA extraction and cumulus cells were cultured up to passage 2 in RCMEP (Stem Cell Research Center, Biostar, Seoul, Republic of Korea) containing 0.2 mmol/L ascorbic acid, 0.09 mmol/L calcium, 5 ng/mL EGF, and 5% fetal bovine serum (FBS) [25]. When the samples had reached about 100% confluency, they were harvested and centrifuged at 1500 rpm for 2 min.…”

Section: Collection Of Immature Oocytes and Cumulus Cellsmentioning

confidence: 99%