Effect of Curcumin on Cisplatin- and Oxaliplatin-Induced Oxidative Stress in Human Embryonic Kidney (HEK) 293 Cells

Abstract

Generation of reactive oxygen species (ROS) is involved in the nephrotoxicity of platinum anticancer drugs. This study involved incubation of human embryonic kidney (HEK) 293 cells in cell culture media supplemented with cisplatin or oxaliplatin in the presence or absence of curcumin, a well-studied antioxidant. Thereafter several indices of oxidative stress have been measured, which included glutathione (GSH), total antioxidant capacity (TAC), and antioxidant enzymes [(superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidases (GPX)]. The impact of platinum drugs on cells viability, lipid peroxidation, and lactate dehydrogenase leakage was also examined. The results show that at both acute (60 min) and chronic (24 h) durations of incubation, cisplatin and oxaliplatin induced oxidative stress as evidenced by significant inhibition of the activities of SOD, CAT, and GPX enzymes as well as significant reduction of the concentrations of GSH and TAC. Curcumin ameliorated the oxidative stress induced by these insults by significantly restoring the measured oxidative indices. Our findings provide evidence that curcumin significantly ameliorates oxidative stress induced by both cisplatin and oxaliplatin in HEK cells.

Acute effects of diesel exhaust particles and cisplatin on oxidative stress in cultured human kidney (HEK 293) cells, and the influence of curcumin thereon

“…Therefore, these concentrations were used to further elaborate the CP and DEP combined treatment in further experiments. Previous work from our research group reported that curcumin has antioxidant properties and protects against cytotoxicity in HEK-293 cells (Waly et al, 2011). Data presented in Fig.…”

“…(Waly et al, 2011(Waly et al, , 2012. However, the possible protective effect of curcumin on DEP, with or without CP, on HEK-293 toxicity has not been, as far as we are aware, investigated so far.…”

Effect of Curcumin on Oxidative Stress in a Model of Turpentine Induced Acute Experimental Inflammation

“…In an acute kidney injury model, oral administration of Curcumin (200 mg kg -1 per day for a week) significantly increased MDA concentration, nitric oxide and carbonylated proteins content in the rat kidney with bilateral renal ischemia, followed by reperfusion (Waly et al, 2011). On the other hand, Curcumin administration improved renal function on mice models (evaluated by blood urea nitrogen and albuminuria), which was associated with a decrease in inflammatory markers and matrix proteins (Trujullo et al, 2013).…”

“…On the other hand, Curcumin administration improved renal function on mice models (evaluated by blood urea nitrogen and albuminuria), which was associated with a decrease in inflammatory markers and matrix proteins (Trujullo et al, 2013). Curcumin significantly improved oxidative stress by reducing TAC and GSH levels, as well as inhibiting the activity of CAT, GPX enzymes and SOD in human embryonic kidney cells (Waly et al, 2011).…”

Down-regulation of thymic stromal lymphopoietin by curcumin

“…1) is the main constituent of the spice turmeric (Curcuma longa) [7,33]. A number of studies have reported that curcumin has antidepressant, antioxidant, antihyperglycemic, antitumor, and anti-inflammatory activities [5,10,19,29,32]. However, the effect of curcumin on the production of TSLP has not yet been clarified.…”