Effect of curcumin on gelatinase A (MMP-2) activity in B16F10 melanoma cells

Banerji, Aniruddha; Chakrabarti, Jayati; Mitra, Aparna; Chatterjee, Amitava

doi:10.1016/j.canlet.2004.02.007

Cited by 78 publications

(48 citation statements)

References 32 publications

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“…By degrading the ECM, MMP-2 and -9 make the metastasis of tumor cells possible. A previous study established that curcumin inhibited the expression and activity of MMP-2/-9 in tumor cells (17)(18)(19)(20). In the present study, we observed that curcumin inhibited the expression and activity of MMP-2/-9 in EC cells.…”

Section: Discussionsupporting

confidence: 67%

Curcumin suppresses migration and invasion of human endometrial carcinoma cells

et al. 2015

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Abstract. Curcumin, a widely used Chinese herbal medicine, has historically been used in anti-cancer therapies. However, the anti-metastatic effect and molecular mechanism of curcumin in endometrial carcinoma (EC) are still poorly understood. The purpose of this study was to detect the anti-metastatic effects of curcumin and the associated mechanism(s) in EC. Based on assays carried out in EC cell lines, it was observed that curcumin inhibited EC cell migration and invasion in vitro. Furthermore, following treatment with curcumin for 24 h, there was a decrease in the expression levels of matrix metalloproteinase (MMP)-2 and -9 as well as proteinase activity in EC cells. Moreover, curcumin treatment significantly decreased the levels of the phosphorylated form of extracellular signalregulated kinase (ERK) 1/2. MEK1 overexpression partially blocked the anti-metastatic effects of curcumin. Combined treatment with ERK inhibitor U0126 and curcumin resulted in a synergistic reduction in MMP-2/-9 expression; the invasive capabilities of HEC-1B cells were also inhibited. In conclusion, curcumin inhibits tumor cell migration and invasion by reducing the expression and activity of MMP-2/9 via the suppression of the ERK signaling pathway, suggesting that curcumin is a potential therapeutic agent for EC.

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Section: Discussionsupporting

confidence: 67%

Curcumin suppresses migration and invasion of human endometrial carcinoma cells

et al. 2015

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“…These modifications contributed to cell detachment followed by cell death. These effects in tumor cells treated with curcumin are triggered mainly by downregulation of focal adhesion kinase (FAK) co-related to detachment-dependent apoptosis (anoikis) [34] or by integrin α6β4 inhibition which is correlated with tumor cell proliferation and anchorage-independent growth. This integrin mobilizes lamellipodia and filopodia, besides it interacts with growth factor receptors, leading to interaction with the actin cytoskeleton [35].…”

Section: Discussionmentioning

confidence: 99%

Bcl-2 family proteins and cytoskeleton changes involved in DM-1 cytotoxic effect on melanoma cells

et al. 2013

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Melanoma is one of the most aggressive types of skin cancer and its incidence rate is still increasing. All existing treatments are minimally effective. Consequently, new therapeutic agents for melanoma treatment should be developed. The DM-1 compound is a curcumin analog that possesses several curcumin characteristics, such as antiproliferative, antitumor and antimetastatic properties. The aim of this study was to evaluate the different signaling pathways involved in the cytotoxic effect of DM-1 on melanoma cells.The apoptotic process and cytoskeletal changes were evaluated by immunoblotting and immunofluorescence, respectively, in melanoma cells.After DM-1 treatment, SK-MEL-5 melanoma cells showed actin filaments disorganization with spicules formation throughout the cytoskeleton and significant reduction of focal adhesion as well as they were present only at cell extremities, conferring a poor connection between the cell and the substrate. Besides this, there was significant filopodium retraction and loss of typical cytoskeleton scaffold. These modifications contributed to cell detachment followed by cell death.Furthermore, DM-1-induced apoptosis was triggered by multiple Bcl-2 proteins involved in both the extrinsic and the intrinsic apoptotic pathways. SK-MEL-5 cells showed a death mechanism mainly by Bcl-2/Bax ratio decrease, whereas A375 cells presented apoptosis induction by Mcl-1 and Bcl-xL downregulation. In SK-MEL-5 and A375 melanoma cells, there were a significant increase in the active form of caspase 9 and the inactive form of the effector caspase 3 was decreased in both cell lines. Expression of cleaved Parp was increased after DM-1 treatment in these melanoma cell lines, demonstrating 2 that the apoptotic process occurred. Altogether, these data elucidate the cellular and molecular mechanisms involved in the cytotoxicity induced by the antitumor agent DM-1 in melanoma cells.

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“…Consistent with this, our study shows more efficient tumor cell extravasation into timp-3-deficient lung. B16 melanoma cells produce significant levels of MMP-2 and MT1-MMP, which have been shown to localize at the tumor-stroma border and enhance their invasive potential (Banerji et al, 2004;Hofmann et al, 2005). The MMP-2 activation seen at 48 h, which is the period of B16F10 cell extravasation, may be particularly important in facilitating extravasation of these cells across the basement membrane in timp-3 À/À lung.…”

Section: Discussionmentioning

confidence: 99%

Enhanced metastatic dissemination to multiple organs by melanoma and lymphoma cells in timp-3−/− mice

et al. 2006

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Identifying versatile inhibitors of metastasis that operate in multiple sites against distinct cancer cell types is important for designing novel therapeutics for metastasis. We show that multiple tissues of timp-3 À/À mice are more susceptible to metastatic colonization. Overall, a 5-14-fold increase in liver and kidney colonization occurred by EL-4 lymphoma cells, and a twofold increase upon targeting B16F10 melanoma cells to the bone or lung of timp-3 À/À mice. There was a general lack of macrophage or neutrophil localization to metastases in the liver, kidney and lung, and of osteoclasts to bone in both genotypes. Analysis of lung showed that proliferation or angiogenesis were unaltered within the metastatic colonies. Lung-trap assays revealed that initial tumor cell trapping was similar in the lung vasculature of timp-3 À/À and wild-type mice. However, more tumor cells were found in timp-3 À/À lungs at 48 and 96 h after tumor cell injection indicating more efficient extravasation and initial proliferation. Activation of pro-MMP-2 was greater in timp-3 À/À lungs at these time points. These data demonstrate TIMP-3 functions to inhibit metastatic dissemination of diverse cancer cells to multiple organs. TIMP-3 regulates MMP-2 activation to limit tumor cell extravasation and subsequent colonization of the lung, without augmenting inflammatory cell response.

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Effect of curcumin on gelatinase A (MMP-2) activity in B16F10 melanoma cells

Cited by 78 publications

References 32 publications

Curcumin suppresses migration and invasion of human endometrial carcinoma cells

Curcumin suppresses migration and invasion of human endometrial carcinoma cells

Bcl-2 family proteins and cytoskeleton changes involved in DM-1 cytotoxic effect on melanoma cells

Enhanced metastatic dissemination to multiple organs by melanoma and lymphoma cells in timp-3−/− mice

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