Effect of curcumin on the expression of Caspase-3 and Bcl-2 in the spleen of diabetic rats

Hashish, Hagar A; Kamal, Rania

doi:10.4103/1596-2393.158923

Cited by 19 publications

(16 citation statements)

References 20 publications

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“…There was significant increase in the expression of the apoptotic marker cleaved caspase-3. Similar results in previous studies was reported as well as decreased expression of the antiapoptotic marker BCl-2 [20] . Reactive oxygen species (ROS) in many target organs that are produced secondary to diabetes can lead to considerable cellular damage and apoptosis when insufficient cytoprotective molecules are available as previously studied as in kidney and liver [28] .…”

Section: Discussionsupporting

confidence: 92%

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A Comparative Study between Ginger and Echinacea Possible Effect on the Albino Rat Spleen of Experimentally Induced Diabetes

Said

Abdel-Aziz

Elhaliem

et al. 2020

Egyptian Journal of Histology

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Section: Discussionsupporting

confidence: 92%

“…Negative control experiments were made by omitting the primary antibody. Palatine tonsil specimens were the positive control [20] .…”

Section: Light Microscopic Studymentioning

confidence: 99%

A Comparative Study between Ginger and Echinacea Possible Effect on the Albino Rat Spleen of Experimentally Induced Diabetes

Said

Abdel-Aziz

Elhaliem

et al. 2020

Egyptian Journal of Histology

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“…Our results are in harmony with a previous study which showed that resveratrol could inhibit apoptosis [52]. Moreover, curcumin decreased the expression of caspase-3 in the spleen of diabetic rats [53]. This can be explained by the ability of both drugs to overcome the depletion of antioxidants and lipid peroxidation which prompt the apoptotic pathways [54,55].…”

Section: Discussionsupporting

confidence: 90%

Natural polyphenols target the TGF-β/caspase-3 signaling pathway in CCl4-induced liver fibrosis in rats

Abu-Baih¹,

Bayoumi

Ibrahim

et al. 2019

Journal of advanced Biomedical and Pharmaceutical Sciences

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Background/Aims: Liver fibrosis presents a worldwide problem. There is an increasing interest in the studying of natural compounds with free radicals scavenging capacity to protect against liver fibrosis. This study investigated the actions of resveratrol and curcumin as two natural polyphenols against CCl 4 -induced liver fibrosis. Materials and Methods: Animals were divided into four groups: Normal control group; CCl 4 only group; Res group (resveratrol + CCl 4 ); Cur group (curcumin + CCl 4 ). Serum ALT and AST were evaluated. Assessment of liver MDA, GSH and catalase was performed. Caspase-3 expression was evaluated by western blotting. Levels of TGF-β1 and TGF-β2 mRNA were determined using qRT-PCR. In addition, liver histopathological sections were examined. Results: We found that serum ALT and AST were elevated by CCl 4 , however, they were significantly reduced by resveratrol and curcumin. Furthermore, CCl 4 induced oxidative stress, stimulated fibrosis and apoptosis. Resveratrol and curcumin significantly attenuated the state of oxidative stress, fibrosis and apoptosis. Conclusion: We conclude that resveratrol and curcumin are natural polyphenols that can protect against liver fibrosis not only via antioxidant, but also via anti-fibrotic and anti-apoptotic potentials.

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“…Feulgen's reaction (B) (X400) Caspase -3 was over expressed in the diabetic control group compared with the normal control group, consistent with previous reports Hashish and Kamal [37] and suggests an increase in apoptotic index. In a study by Kamel et al [38], it was also found out that Caspase -3 level was significantly elevated in diabetic rat pancreas while treatment with curcumin resulted in a significant reduction of the enzyme.…”

Section: Plate 1 Photomicrograph Of Pancreas Of Normal Control Animals Stained With H and E (A) Andsupporting

confidence: 90%

Administration of Combined Methanolic Leaf Extracts of Vernonia amygdalina and Gongronema latifolium Enhanced Glut 2 Expression in the Pancreas and Downregulates Serum Caspase 3 Activity of Streptozotocin-Induced Diabetic Wistar Rats

Akpaso

Orie

Ebong

2019

JAMMR

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Aim: The study evaluated the effects of the combined extracts of Vernonia amygdalina (VA) and Gongronema latifolium (GL) on pancreatic GLUT 2 expression and caspase 3 activity in streptozotocin (STZ, 45 mg/Kg)-induced diabetic rats. Study Design: Fifteen Albino rats were used for the study and were placed in 3 groups of 5 rats each: A - normal control, B – Diabetic control and C – experimental group. Place and Duration of Study: The study was carried out in the department of Anatomy, University of Calabar. Duration: 6 months. Methodology: Half of the diabetic rats were treated with VA+GL (400mg/kg, ratio 1:1, DE group) for 28 days, while the other half was untreated and served as diabetic control (DC). Normal control (NC) rats were untreated. After 28 days, the rats were sacrificed and their blood glucose, serum GLUT 2 and caspase 3 activity were measured. Histochemical evaluation of the pancreas was also carried out. Results: Blood glucose concentrations for the 3 groups were 60.31±7.28, 257.00±4.43, and 116.60±10.11 mg/dl for NC, DC and DE respectively. This represented a 4-fold increase in the DC compared with NC and a significant amelioration in the extract-treated DE group compared with DC group. Serum GLUT 2 concentrations were 70 ng/ml in NC, dropped to 8 ng/ml (p<0.05) in the DC and recovered to 20 ng/ml in DE (p<0.05). Serum caspase was 3.2 ng/ml for NC, increased to 8.5 ng/ml in DC (p<0.05) and reduced to 1.8 ng/ml in DE (p<0.05). The histology of the pancreas showed distorted, degenerated and shrunken β-cells mass in DC compared with NC and DE groups. The DE group showed clear signs of regeneration of the islet cells which was corroborated by positive Feulgen’s reaction compared with the DC group. Conclusion: The data suggests that the combined VA+GL extract has the potential to effectively reverse pancreatic damage in diabetes.

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Effect of curcumin on the expression of Caspase-3 and Bcl-2 in the spleen of diabetic rats

Cited by 19 publications

References 20 publications

A Comparative Study between Ginger and Echinacea Possible Effect on the Albino Rat Spleen of Experimentally Induced Diabetes

A Comparative Study between Ginger and Echinacea Possible Effect on the Albino Rat Spleen of Experimentally Induced Diabetes

Natural polyphenols target the TGF-β/caspase-3 signaling pathway in CCl4-induced liver fibrosis in rats

Administration of Combined Methanolic Leaf Extracts of Vernonia amygdalina and Gongronema latifolium Enhanced Glut 2 Expression in the Pancreas and Downregulates Serum Caspase 3 Activity of Streptozotocin-Induced Diabetic Wistar Rats

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