Effect of degumming methods on structural characteristics and properties of regenerated silk

Kim, Hyun Ju; Kim, Moo Kon; Lee, Ki Hoon; Nho, Si Kab; Han, Myung Sae; Um, In Chul

doi:10.1016/j.ijbiomac.2017.06.019

Cited by 78 publications

(66 citation statements)

References 51 publications

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“…Longer degumming times result in broader molecular weight distributions, without affecting the ß sheet content (Nultsch & Germershaus, 2017). Differences in molecular weight distributions result in varying mechanical properties (H. H. Kim et al, 2016;H. J. Kim, Kim, et al, 2017) and degradation times (Nultsch & Germershaus, 2017).…”

Section: Silk Fibroin Extractionmentioning

confidence: 99%

“…Degumming is most commonly done in a dilute sodium carbonate solution (Nultsch & Germershaus, ; Sofia, McCarthy, Gronowicz, & Kaplan, ); however, other degumming reagents have been studied such as urea, citric acid, and sodium oleate (H. J. Kim et al, ). Silk fibers have a higher maximum load, fracture energy, and maximum tensile strain when degummed by urea (Wang & Zhang, ).…”

Section: Introductionmentioning

confidence: 99%

“…Longer degumming times result in broader molecular weight distributions, without affecting the ß sheet content (Nultsch & Germershaus, ). Differences in molecular weight distributions result in varying mechanical properties (H. H. Kim et al, ; H. J. Kim, Kim, et al, ) and degradation times (Nultsch & Germershaus, ). For drug delivery approaches, it was found that an increase in degumming time resulted in an increase in the release of neutral charged reagents (Nultsch & Germershaus, ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microscopic considerations for optimizing silk biomaterials

DeBari

Abbott

2018

WIREs Nanomed Nanobiotechnol

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Silk is an especially appealing biomaterial due to its adaptable mechanical properties, allowing it to be used in a wide range of tissue engineering applications. However, processing conditions play a critical role in determining silk's mechanical properties, biodegradability, and biocompatibility. While bulk properties of silk have been widely explored, focusing on microscopic features is becoming increasingly important, as modifications at this scale largely affect the resulting regenerative properties of the biomaterial. Structural changes caused by the silk source, extraction, and processing should be carefully considered, as they will affect the biocompatibility and degradability of silk fibroin. Processing techniques and physical properties of silk that make it an ideal material for many biomedical applications will be explored. This article is categorized under: Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement Implantable Materials and Surgical Technologies > Nanomaterials and Implants.

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Section: Silk Fibroin Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Microscopic considerations for optimizing silk biomaterials

DeBari

Abbott

2018

WIREs Nanomed Nanobiotechnol

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“…In general, a hot aqueous medium is used as extracting medium, preferably alkaline, in which SS is soluble and thus is separated from SF fibers, which remain essentially insoluble under these conditions. Several degumming methods have been reviewed recently showing the effect of this critical processing step on the structure and properties of SF for multiple applications [15][16][17][18][19][20][21][22][23][24]. Among the different methods, the use of an autoclave in different configurations is frequently mentioned: in an aqueous solution subjected to 121 °C for 20 minutes [15] or using water vapor generated in the autoclave [17].…”

Section: Introductionmentioning

confidence: 99%

“…Several degumming methods have been reviewed recently showing the effect of this critical processing step on the structure and properties of SF for multiple applications [15][16][17][18][19][20][21][22][23][24]. Among the different methods, the use of an autoclave in different configurations is frequently mentioned: in an aqueous solution subjected to 121 °C for 20 minutes [15] or using water vapor generated in the autoclave [17]. Although this method presents a lower yield than the protocols that use alkaline boiling, it has the advantage of lower production costs while maintaining acceptable the mechanical characteristics of SF fibers and is very useful when trying to recover salt-free SS by spray-drying [25,26].…”

Section: Introductionmentioning

confidence: 99%

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Carissimi¹,

Lozano-Pérez²,

Montalbán³

et al. 2019

Preprint

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In recent years, numerous research studies have shown the excellent characteristics of silk fibroin nanoparticles as a vehicle for drugs delivery and it is foreseeable that their production could reach industrial scale in the coming years. For this reason, it is essential to know all the parameters that affect the formation of nanoparticles in order to standardize the process. Several studies have stated that the process used for sericin removal (degumming) from silk cocoons has a strong impact in the silk fibroin integrity and their mechanical properties after processing it into biomaterials. In this work, silk cocoons were degummed following four standard methods: autoclaving, short alkaline (Na2CO3) boiling, long alkaline (Na2CO3) boiling and ultrasounds. The resultant silk fibroin fibers were dissolved in the ionic liquid 1-ethyl-3-methylimidazolium acetate and used for nanoparticle synthesis by rapid desolvation in polar organic solvents. The relative efficiencies of the degumming processes and the integrity of the resulting fibroin fibers obtained were analyzed by weight loss, optical microscopy, thermogravimetric analysis, infrared spectroscopy and SDS-PAGE. Particle sizes and morphology were analyzed by Dynamic Light Scattering and Field Emission Scanning Electronic Microscopy. The results showed that the different treatments had a remarkable impact on the integrity of the silk fibroin chains, as confirmed by gel electrophoresis which can be correlated with particle mean size and size distribution changes. The study confirm that all the parameters of the process must be controlled in order to reach an optimum reproducibility of the nanoparticle production.

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Stretchable and Heat‐Resistant Protein‐Based Electronic Skin for Human Thermoregulation

Huang

Zhang

Qiu

et al. 2020

Adv Funct Materials

125

116

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Silk protein is one of the a promising materials for on-skin and implantable electronic devices due to its biodegradability and biocompatibility. However, its intrinsic brittleness as well as poor thermal stability limits its applications. In this work, robust and heat-resistant silk fibroin composite membranes (SFCMs) are synthesized by mesoscopic doping of regenerated silk fibroin (SF) via the strong interactions between SF and polyurethane. Surprisingly, the obtained SFCMs can endure the tensile test (>200%) and thermal treatment (up to 160 °C). Attributed to these advantages, traditional micromachining techniques, such as inkjet printing, can be carried out to print flexible circuits on such protein substrate. Based on this, Ag nanofibers (NFs) and Pt NFs networks are successfully constructed on both sides of the SFCMs to function as heaters and temperature sensors, respectively. Furthermore, the integrated protein-based electronic skin (PBES) exhibits high thermal stability and temperature sensitivity (0.205% °C −1 ). Heating and temperature distribution detection are realized by array-type PBES, contributing to potential applications in dredging of the blood vessel for alleviating arthritis. This PBES is also inflammation-free and air-permeable so that it can directly be laminated onto human skin for long-term thermal management.

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Effect of degumming methods on structural characteristics and properties of regenerated silk

Cited by 78 publications

References 51 publications

Microscopic considerations for optimizing silk biomaterials

Microscopic considerations for optimizing silk biomaterials

Revealing the Influence of the Degumming Process in the Properties of Silk Fibroin Nanoparticles

Stretchable and Heat‐Resistant Protein‐Based Electronic Skin for Human Thermoregulation

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