Effect of dehydrated carrot on the antioxidant enzymes and micronuclei formation in rats challenged with dimethylhydrazine

Khanum, Farhath; Sudarshanakrishna, K R; Viswanathan, K.; Santhanam, K.

doi:10.1016/s0271-5317(00)00165-2

Cited by 7 publications

(5 citation statements)

References 9 publications

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“…Aloe vera, a medicinal plant belonging to the Asphodelaceae family, has been demonstrated to be capable of combating oxidative stress (22). The natural products obtained from it are divided into two groups: gel and latex.…”

Section: Discussionmentioning

confidence: 99%

Effects of Methanolic Extract of Aloe vera on C-Reactive Protein and White Blood Cell Count of Wistar Rats With Inflammation

Anyiam,

Irondi,

Obi

et al. 2023

Avicenna J Med Biochem

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Background: It has been known for centuries that plants and plant-derived chemicals are useful in treating diseases, with Aloe vera being one of such biologically active plants. A significant role is played by the C-reactive Protein (CRP) in many inflammatory conditions, while leukocytes play an integral role in the immune system's response to infectious diseases. Objectives: This study explored the potential anti-inflammatory effects of Aloe vera methanolic extract on the total white blood cell (TWBC) count and CRP levels in wistar rats administered formalin. Methods: A total of 25 wistar rats were divided into five groups (A to E), with group A serving as the control and the other groups being administered formalin to induce inflammation, followed with increasing doses of Aloe vera extract. The CRP level was measured using an enzyme-linked immunosorbent assay (ELISA) kit, and the TWBC count was determined using a Sysmex Haematology Auto-analyzer. Results: Research findings showed that 200 mg of Aloe vera methanolic extract effectively reduced the C-reactive protein (0.056±0.0027) (p = 0.04), while 600 mg of the extract significantly increased the TWBC count (13.920±4.757) (p = 0.03). Conclusion: These findings suggest that Aloe vera extract may be an effective natural supplement for supporting the immune system function and reducing inflammation in the body. However, further research is needed to fully understand the underlying mechanisms for these effects and to determine the optimal dosages and potential side effects.

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Section: Discussionmentioning

confidence: 99%

Effects of Methanolic Extract of Aloe vera on C-Reactive Protein and White Blood Cell Count of Wistar Rats With Inflammation

Anyiam,

Irondi,

Obi

et al. 2023

Avicenna J Med Biochem

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“…In earlier studies, DMH tested negative but these single‐dose studies where either restricted by the top dose or the route of administration [Trzos et al, 1978; Albanese et al, 1988]. In comparison, a number of studies with DMH employing higher dose levels produced positive results by a variety of administrations [Wakata et al, 1998; Anilakumar et al, 2000; Khanum et al, 2000]. Multi‐dose studies at lower doses usually produced negative results [Zhurkov et al, 1996; Hamada et al, 2001a].…”

Section: Discussionmentioning

confidence: 99%

The rat gut micronucleus assay: A good choice for alternative in vivo genetic toxicology testing strategies

Coffing

Engel

Dickinson

et al. 2010

Environ and Mol Mutagen

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The in vivo bone marrow (BM) micronucleus assay is one of the three tests in the standard test battery to assess the genotoxic potential of a pharmaceutical candidate. In some cases, depending on results of in vitro studies, the route of administration or the degree of systemic exposure, in vivo assessment of genotoxicity in the BM alone may not be sufficient. Based on the potential for high gut exposures to orally administered compounds with low systemic exposures as well as the potential susceptibility of rapidly dividing cells of the intestinal tissues, we have developed a modified technique for evaluating micronuclei formation in both the duodenum and colon of rats based on earlier publications. Adult male Sprague Dawley rats were treated once daily for 2 days with either vehicle control or with the test articles acetyl salicylic acid (ASA), carbendazim (CAR), cyclophosphamide (CP), dimethylhydrazine (DMH), mitomycin C (MMC) or vinblastine sulfate (VIN). The duodenum, colon, and BM were harvested, processed, and analyzed for micronucleus induction. Results from these studies demonstrated differences in the susceptibility for different test compounds in the three tissues tested. When MMC and VIN were dosed by different routes at the same dose levels both compounds produced positive results in all three tissues by intraperitoneal injection but not oral administration. These studies suggest that overall the GI micronucleus assay might be a useful tool for clastogenic and aneugenic compounds that are expected to produce high sustained concentrations in the gastrointestinal tract with little systemic exposure.

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“…Proximal compounds were determined through the AOAC (Association of Official Analytical Chemists) methods [46], while the Kjeldahl method was used for protein determination [47].…”

Section: Proximate Compositionmentioning

confidence: 99%

Fresh-Cut Mangoes: How to Increase Shelf Life by Using Neem Oil Edible Coating

et al. 2022

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The mango is the most widely cultivated tropical fruit. Fresh-cut mango is very useful, but it is very perishable. The purpose of this study was to highlight the effects of neem oil on fresh-cut mango fruits kept for 9 days at 4 ± 1 °C and 80 ± 5% relative humidity. The neem plant (Azadirachta indica) has numerous antioxidant and antibacterial properties. Despite this, very few studies have been carried out on neem oil added to edible coatings (EC) to retard ripening processes. Two formulations were tested: EC1 (hydroxypropyl methylcellulose + CaCl2) and EC2 (hydroxypropyl methylcellulose + CaCl2 + neem oil), both compared with an untreated sample (control). Physicochemical, microbial, proximate and sensory analyses were carried out. Neem oil reduced loss of firmness and colour, while hydroxypropyl methylcellulose and CaCl2 reduced normal cell degradation (weight loss and soluble solids content). Microbiological investigation showed that the EC2 inhibited the development of the main spoilage bacteria during the entire storage period, prolonging the preservation of fresh-cut fruits. The sensory analysis showed a rapid degradation after 5 d in the control sample, while the EC2 was the best.

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Effect of dehydrated carrot on the antioxidant enzymes and micronuclei formation in rats challenged with dimethylhydrazine

Cited by 7 publications

References 9 publications

Effects of Methanolic Extract of Aloe vera on C-Reactive Protein and White Blood Cell Count of Wistar Rats With Inflammation

Effects of Methanolic Extract of Aloe vera on C-Reactive Protein and White Blood Cell Count of Wistar Rats With Inflammation

The rat gut micronucleus assay: A good choice for alternative in vivo genetic toxicology testing strategies

Fresh-Cut Mangoes: How to Increase Shelf Life by Using Neem Oil Edible Coating

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