Effect of demographic location on Phlebodiumdecumanum (Willd.) J. Sm. for its phytoconstituents and establishment of antioxidant and novel anthelmintic activity of its aqueous and methanolic leaf extracts

Abstract

A fern, Phlebodium decumanum (Willd.) J.Sm. (PD) belongs to the family Polypodiaceae. It is a creeping, densely hairy or scaly rhizome bearing fronds at intervals along its length. It is native to tropical and subtropical regions of the America. The present study was investigated to establish the presence of phytoconstituents in leaf samples collected from three different geographical zones (Bengaluru, Nasik, and Munnar) and their impact on antioxidant and anthelmintic activity. Aqueous and methanol leaf extracts were used as solvent for extraction (conventional Soxhlet method) and results were compared to established novel application of fern species. Results revealed presence of alakloids, polyphenols, terpenoids, flavonoids, etc., upon various chemical tests. Thereafter, antioxidant study was established with DPPH and FRAP assay method. The IC 50 value for methanol extract showed higher (210.24 µg/ml) for Munnar sample than the other two samples. An anthelmintic activity was determined by compared with Albendazole standard (25 mg/ml). Results revealed significant a nthelmintic activity (p<0.01 ) for methanol extra ct of Munnar sample in terms of the time taken for paralysis and death study.

Effect of plant hormones and zinc sulphate on rooting and callus induction in in vitro propagated Coscinium fenestratum (Gaertn.) Colebr. stem and their role in estimation of secondary metabolites

“…Further, it is also revealed that percentage yield of extract also higher in methanol extract than others (Arawwawala and Wickramaarachchi, 2012;Akowuah et al, 2014) and the same trend followed in the present study where methanol extract of callus showed higher percentage of yield than aqueous extract. It is also proved that content of active constituents are also depends on the percentage of yield (Dent et al, 2013;Das et al, 2016;Das et al, 2017). Our present study is also reported the similar results that influence direct correlation with the amount content of the active constituents.…”

In vitro antioxidant and free-radical scavenging activities of polar leaf extracts of Vernonia amygdalina

“…Hence, plants containing useful phytochemicals may supplement the of the human body by acting as natural antioxidants. These phytoconstituents depend on the geographical location where the plant material collected [ 29 ] and the season as well as the maturity of leaves [ 30 ]. The chemical profile and antioxidant activities of V. amygdalina grown in the study area is not reported yet.…”

Phytochemical screening, metallic ion content and from its impact on Anti psoriasis activity of Calendula officinalis and Phlebodium decumanum aqueous leaf extracts in animal experiment model

“…But the main concern is to discovery of new drugs from the plant extracts which is more potent than the extracts against any kind of human health hazards. The main reason behind the selection of these two plants leaf because both the plants enhanced the immunity and act strongly against any infections due to their high antioxidant activities 36,10 . It was revealed that selected plant extracts showed significant antipsoriasis activity due to the presence of important secondary metabolites (discussed earlier in introduction part).…”

“…Scientifically leaves are also claimed as having antimicrobial activity 7 , hepatoprotective activity 8 and wound healing activity 9 . Thereafter Phlebodium decumanum (PD) (family Polypodiaceae), commonly known as ornamental fern, is abundantly available in damp region of many places in India 10 . The leaves contains many chemicals viz.…”

“…Thereafter literature survey revealed the presence of anti-inflammatory activity 12 , antioxidant 13 , wound healing, improve immune system 14 , antimicrobial, anthelmintic 10 etc. Henceforth therapeutic activities of plant constituents are greatly depend on the soil fertility, climatic conditions and content of metal ions in the accumulated plant parts [15][16][17][18][19] .…”