Effect of dentinal fluid on enamel permeability under simulated pulpal pressure

Chinajitphan, Narinee; Chunhacheevachaloke, Ekachai; Ajcharanukul, Orapin

doi:10.1016/j.archoralbio.2018.12.010

Cited by 8 publications

(7 citation statements)

References 36 publications

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“…Thus, we first investigated the key changes that occur following the IP treatment of natural WSL by using our in situ model of generating enamel fluid under simulating pulpal pressure (Tanapitchpong et al 2020). A previous study showed that dentinal fluid could enhance the involvement of enamel fluid in the process of enamel remineralization, particularly after etching (Chinajitphan et al 2019). Therefore, Ringer's solution used in this study functioned as tissue fluid, providing minerals such as Ca and P necessary for creating subsurface remineralization during the inward flow of F following the IP.…”

Section: Discussionmentioning

confidence: 99%

“…Due to differences in the experimental designs, most data revealed some positive results, whereas some recent findings demonstrated indifferent outcomes (Kim et al 2009; Lee et al 2010). Regarding the studies of enamel permeability, previous research demonstrated that enamel fluid in vivo was ultrafiltrated partly from the dentinal fluid, whose presence plays a role in enamel remineralization (Chinajitphan et al 2019; Tanapitchpong et al 2020). Therefore, the in vitro study of enamel remineralization should be conducted under simulated pulpal pressure.…”

Section: Introductionmentioning

confidence: 99%

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Enamel Remineralization and Crystallization after Fluoride Iontophoresis

et al. 2022

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Early caries lesions consist of noncavitated subsurface demineralization caused by the dissolution of hydroxyapatite from the surface to the subsurface area of the enamel. Such lesions cannot be remineralized effectively by the conventional treatment. Thus, there is a need for a noninvasive technique capable of delivering the remineralizing agent to subsurface sites. For this purpose, fluoride iontophoresis (IP) using weak currents has been investigated with some conflicting results and no information on the crystal structure and composition. Because enamel remineralization involves the role of fluid from dentin, the presence of enamel fluid is necessary to determine the repair associated with the physiological condition. This study aimed to investigate structural and compositional characteristics, including the remineralizing effect of 5% sodium fluoride (NaF) IP with different polarities, cathodal iontophoresis (CIP), and anodal iontophoresis (AIP) for the treatment of natural enamel caries under simulated pulpal pressure. A bulk measurement of the crystal structure inside the lesion was first determined using calcium (Ca) K-edge X-ray absorption spectroscopy. IP with both polarities significantly promoted subsurface remineralization. The CIP generated a significant increase in the Ca/phosphorus ratio, and fluoride at the surface lesion significantly correlated with higher mineral density (MD) and more strengthening crystal structure of the lesion volume, while the lesion’s MD and other impurities at the lesion surface, mostly the carbonate ions, affected the significant increase in MD with the unchanged structure of the lesion volume after AIP. The CIP of NaF is an ideal method for rapid enamel remineralization and recrystallization of fluoroapatite/fluorohydroxyapatite.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enamel Remineralization and Crystallization after Fluoride Iontophoresis

et al. 2022

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“…Then, the APC base was screwed to seal the system. A hydrostatic pressure at 20 cm/H 2 O (i.e., 14.7 mm Hg), representing normal pulp tissue fluid pressure (Roberts and Pashley 2012; Chinajitphan et al 2019), was applied and the system was incubated at 37°C and 5% CO 2 for 12 h. After that, the base was removed, the culture medium was changed, and the inferior opening was covered with a filter cap (polystyrene, 0.45 μm; Corning). The cells were then incubated for an additional 12 h with no pressure to allow cell oxygenation.…”

Section: Methodsmentioning

confidence: 99%

Chitosan-Calcium-Simvastatin Scaffold as an Inductive Cell-Free Platform

Soares¹,

Bordini

Bronze‐Uhle³

et al. 2021

J Dent Res

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The development of biomaterials based on the combination of biopolymers with bioactive compounds to develop delivery systems capable of modulating dentin regeneration mediated by resident cells is the goal of current biology-based strategies for regenerative dentistry. In this article, the bioactive potential of a simvastatin (SV)–releasing chitosan-calcium-hydroxide (CH-Ca) scaffold was assessed. After the incorporation of SV into CH-Ca, characterization of the scaffold was performed. Dental pulp cells (DPCs) were seeded onto scaffolds for the assessment of cytocompatibility, and odontoblastic differentiation was evaluated in a microenvironment surrounded by dentin. Thereafter, the cell-free scaffold was adapted to dentin discs positioned in artificial pulp chambers in direct contact with a 3-dimensional (3D) culture of DPCs, and the system was sealed to simulate internal pressure at 20 cm/H2O. In vivo experiments with cell-free scaffolds were performed in rats’ calvaria defects. Fourier-transform infrared spectroscopy spectra proved incorporation of Ca and SV into the scaffold structure. Ca and SV were released upon immersion in a neutral environment. Viable DPCs were able to spread and proliferate on the scaffold over 14 d. Odontoblastic differentiation occurred in the DPC/scaffold constructs in contact with dentin, in which SV supplementation promoted odontoblastic marker overexpression and enhanced mineralized matrix deposition. The chemoattractant potential of the CH-Ca scaffold was improved by SV, with numerous viable and dentin sialoprotein–positive cells from the 3D culture being observed on its surface. Cells at 3D culture featured increased gene expression of odontoblastic markers in contact with the SV-enriched CH-Ca scaffold. CH-Ca-SV led to intense mineralization in vivo, presenting mineralization foci inside its structure. In conclusion, the CH-Ca-SV scaffold induces differentiation of DPCs into a highly mineralizing phenotype in the presence of dentin, creating a microenvironment capable of attracting pulp cells to its surface and inducing the overexpression of odontoblastic markers in a cell-homing strategy.

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“…The pulp chamber was then irrigated with water from a triple syringe for 10 min and filled with 2.5 N sodium hydroxide for 3 days to remove any remaining tissue and odontoblasts [4] . The in vitro experiment was conducted by using the model for fluid flow measurement [5] . After the pressure in the pulp cavity was set at 0, +20, +100, and +200 mm Hg, the replica was made from the enamel surface of the crown at 5 min following each of the applied pressure by using the same procedure as that in vivo.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

“…For the fluid flow measurement group, a recently extracted tooth was prepared as above. A similar setting for the measurement of the fluid flow rate through the tooth crown was performed following the applications of the same series of positive pulpal pressures [5] . However, four teeth of 3 subjects were arranged for the recording of fluid conductance, while the replica impression at the mid buccal surface was also taken simultaneously under each of the applied pulpal pressure.…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Effect of the pulpal hydrostatic pressure on the morphological data of the fluid droplets emerging from dental enamel in human teeth

2020

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Enamel fluid in human teeth plays an important role in the permeability and mechanical property of the enamel. It can be invetigated as fluid droplets at the enamel surface by using the replica technique. The experiments were done on 17 premolars of 10 subjects (aged 10–25 yrs) to be extracted during orthodontic treatment. Teeth were divided into 2 groups. In the first group ( n = 11), the fluid accumulated on the mid buccal of the enamel surface was recorded with the impression material both in vivo and in vitro. In vitro replicas were obtained when the pressure in the pulp cavity held at 0, 20, 100, and 200 mmHg above atmospheric. They were examined by a scanning electron microscope. For the other group ( n = 10), each tooth was prepared for fluid flow measurement during which the same set of pulpal pressures was applied as mentioned in the other study [1] . However, four teeth of 3 subjects were arranged for the recording of fluid conductance, while the replica impression at the mid buccal surface was also taken simultaneously under each of the applied pulpal pressure. This dataset describes subjects’ baseline characteristics, including their corresponding records of the droplets’ observations both in vivo and in vitro . Also, data of in vitro fluid flow measurements were detailed according to the applied pulpal pressure. The patterns of changes of the fluid flow rates and the droplets’ numbers provided in this dataset can be used to validate tests of agents affecting the structure and permeability of the enamel.

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Effect of dentinal fluid on enamel permeability under simulated pulpal pressure

Cited by 8 publications

References 36 publications

Enamel Remineralization and Crystallization after Fluoride Iontophoresis

Enamel Remineralization and Crystallization after Fluoride Iontophoresis

Chitosan-Calcium-Simvastatin Scaffold as an Inductive Cell-Free Platform

Effect of the pulpal hydrostatic pressure on the morphological data of the fluid droplets emerging from dental enamel in human teeth

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