Effect of dexamethasone on polypeptides synthesised in polymorphonuclear leucocytes

Blowers, L. E.; Jayson, M. I. V.; Jasani, M K

doi:10.1016/0014-5793(85)80293-3

Cited by 8 publications

(2 citation statements)

References 14 publications

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“…It is also consistent with the effectiveness of relatively short exposures to corticosteroids to cause complete suppression Of 02 production (e.g., 30 min). The de novo synthesis of proteins that has also been suggested as a mechanism of corticosteroid-induced PMN suppression occurs after relatively prolonged times of exposure (2,20). It also provides additional evidence that the suppressive effects of corticosteroids may be due to a direct downregulatory action on specific surface receptors and subsequently on cytoplasmic messengers.…”

Section: Discussionmentioning

confidence: 81%

Prevention of corticosteroid-induced suppression of human polymorphonuclear leukocyte-induced damage of Aspergillus fumigatus hyphae by granulocyte colony-stimulating factor and gamma interferon

et al. 1993

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Neutrophils (PMNs) are a critical line of defense against Aspergil1usfinmigatus infection. Increased frequency of invasive aspergillosis has been observed in patients receiving corticosteroids, suggesting a deleterious effect of these compounds on PMN antifungal function. To investigate this hypothesis and to determine the potential preventive utility of granulocyte colony-stimulating factor (G-CSF) and gamma interferon (IFN-y), the effects of hydrocortisone (HCS) and dexamethasone (DXS) on PMN-induced damage of Aspergilusfumigatus hyphae were studied with or without pretreatment of PMNs with G-CSF and IFN-y. PMNs treated with HCS (23,000 ,uM) or DXS (210 ,uM) during a 2-h colorimetric tetrazolium metabolic assay (using methylthiotetrazolium) showed suppressed percentage of hyphal damage (P < 0.02). In addition, both HCS (.30 ,M) and DXS (>1 ,M) significantly suppressed oxidative burst measured as superoxide anion release by PMNs in response to opsonized and nonopsonized hyphae as well as to N-formylmethionyl leucyl phenylalanine.

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Section: Discussionmentioning

confidence: 81%

Prevention of corticosteroid-induced suppression of human polymorphonuclear leukocyte-induced damage of Aspergillus fumigatus hyphae by granulocyte colony-stimulating factor and gamma interferon

et al. 1993

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“…Under these conditions, potential roles for collagenase autoactivation, substrate-dependent activation, or membrane-associated collagenase activity would not have been assessed, but do deserve consideration [6,7]. In addition, neutrophils are generally considered to be devoid of any biosynthetic activity, but they are able to synthesize a number of proteins including plasminogen activator [43][44][45]. This serine proteinase can cleave the plasma protein plasminogen to form plasmin.…”

Section: Could Other Factors Play a Role In Collagenase Activation?mentioning

confidence: 99%

Collagenolytic metalloenzymes of the human neutrophil

Weiss

Peppin

1986

Biochemical Pharmacology

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Factors Secreted by Untreated and Hydrocortisone-Treated Monocytes That Modulate Neutrophil Function

Frank

Roth

1986

Journal of Leukocyte Biology

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When incubated for 1.0 hr with neutrophils isolated from bovine peripheral blood, hydrocortisone (0.05, 0.5, or 5.0 micrograms/ml) had no significant effect on their ability to ingest Staphylococcus aureus, to reduce nitroblue tetrazolium (NBT) or iodinate protein, or to mediate antibody-dependent cell-mediated cytotoxicity (ADCC) against chicken erythrocytes. Random migration was significantly enhanced by the highest concentration of hydrocortisone (HC) but was unaffected by the lower concentrations. Resting bovine monocytes were incubated for 24 hr in medium with or without added hydrocortisone (0.05, 0.5, or 5.0 micrograms/ml). Purified bovine neutrophils were then incubated for 1.0 hr with the resulting monocyte supernatant (MS). The MS from untreated monocytes significantly enhanced neutrophil ADCC but did not affect the other neutrophil functions evaluated. Supernatants from monocytes incubated with 0.5 or 5.0 micrograms/ml hydrocortisone (HC-treated MS) failed to enhance neutrophil ADCC but did enhance neutrophil random migration under agarose. The other parameters of neutrophil function were unaffected. Production of the factors in MS was reduced by inhibition of protein synthesis in monocytes. Their activity was reduced by exposure of MS to proteolytic enzymes, suggesting that the monocyte factors were polypeptides or proteins. Protein synthesis by the neutrophil was not necessary for its response to the monocyte-produced ADCC-enhancing factor but was necessary for its response to the HC-induced monocyte factor. The results suggest that the antiinflammatory effects of glucocorticoids may be partially mediated by factors released by monocytes.

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Effect of dexamethasone on polypeptides synthesised in polymorphonuclear leucocytes

Cited by 8 publications

References 14 publications

Prevention of corticosteroid-induced suppression of human polymorphonuclear leukocyte-induced damage of Aspergillus fumigatus hyphae by granulocyte colony-stimulating factor and gamma interferon

Prevention of corticosteroid-induced suppression of human polymorphonuclear leukocyte-induced damage of Aspergillus fumigatus hyphae by granulocyte colony-stimulating factor and gamma interferon

Collagenolytic metalloenzymes of the human neutrophil

Factors Secreted by Untreated and Hydrocortisone-Treated Monocytes That Modulate Neutrophil Function

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