Effect of diabetes on exosomal miRNA profile in patients with obesity

Kim, Hyoshik; Bae, Yun-Ui; Lee, Haekyung; Jeon, Jin Seok; Noh, Hyunjin; Han, Dong Cheol; Byun, Dong Won; Kim, Sang Hyun; Park, Hyeong Kyu; Ryu, Seongho; Kwon, Soon Hyo

doi:10.1136/bmjdrc-2020-001403

Cited by 32 publications

(37 citation statements)

References 44 publications

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“…Furthermore, a recent prospective study demonstrated alteration of expression of 25 exosomal miRNA levels between patients with obesity with DM and patients with obesity without DM, highlighting affection of exosomal miRNAs by dysregulated glucose metabolism in patients with obesity. This supports their use as potential biomarker and therapeutic target in patients with obesity with or without DM (H. Kim et al, 2020). On the contrary, human MSC-derived exosomes has been found to be rich in glucose metabolism-associated proteins that promote glucose transporter 4 (GLUT4) expression, increase glycogen storage in liver to maintain glucose homeostasis, inhibit β-cell apoptosis, and improve peripheral insulin sensitivity in type 2 DM (Y. .…”

Section: Exosomes In Covid-19mentioning

confidence: 84%

Exosomes: Isolation, characterization, and biomedical applications

Alzhrani

Alanazi

Alsharif

et al. 2021

Cell Biology International

116

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Exosomes are nano‐sized bioactive vesicles of 30–150 nm in diameter. They are secreted by exocytosis of nearly all type of cells in to the extracellular fluid. Thereby, they can be found in many biological fluids. Exosomes regulate intracellular communication between cells via delivery of their cargo which include lipids, proteins, and nucleic acid. Many desirable features of exosomes made them promising candidates in several therapeutic applications. In this review, we discuss the use of exosomes as diagnostic tools and their possible biomedical applications. Additionally, current techniques used for isolation, purification, and characterization of exosomes from both biological fluids and in vitro cell cultures were discussed.

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Section: Exosomes In Covid-19mentioning

confidence: 84%

Exosomes: Isolation, characterization, and biomedical applications

Alzhrani

Alanazi

Alsharif

et al. 2021

Cell Biology International

116

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“…These exosomal miRNAs can be taken up by recipient cells, and in turn, they subsequently modulate recipient cells (Zhang et al, 2015). Exosomal miRNAs have a significant influence on a variety of diseases including cancer, diabetes, and cardiovascular disease (Chen et al, 2014; Emanueli et al, 2015; Kim et al, 2020). For instance, exosomal miR‐21 has been significantly correlated to liver metastasis and prognosis of colorectal cancer (Zhu, Huang, et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Exosomes from human umbilical cord mesenchymal stem cells inhibit ROS production and cell apoptosis in human articular chondrocytes via the miR‐100‐5p/NOX4 axis

Wang

Cai

et al. 2021

Cell Biology International

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Cyclic strain‐induced chondrocyte damage is actively involved in the pathogenesis of osteoarthritis and arthritis. MicroRNAs (miRNAs) carried by exosomes have been implicated in various diseases. However, the role of miR‐100‐5p in cyclic strain‐induced chondrocyte damage remains to be elucidated. miR‐100‐5p and NADPH oxidase 4 (NOX4) were silenced or overexpressed in human primary articular chondrocytes. PKH‐67 Dye was used to trace exosome endocytosis. Reactive oxygen species (ROS) production was monitored using DCFH‐DA. Cell apoptosis was measured using a flow cytometer. Quantitative RT‐PCR and Western blots were used to evaluate gene expression. Cyclic strain promoted ROS production and apoptosis in primary articular chondrocytes in a time‐dependent manner. HucMSCs‐derived exosomal miR‐100‐5p inhibited cyclic strain‐induced ROS production and apoptosis in primary articular chondrocytes. miR‐100‐5p directly targeted NOX4. Overexpressing NOX4 attenuated hucMSCs‐derived exosomes‐mediated protective effects in primary articular chondrocytes. Cyclic strain promotes ROS production and apoptosis in primary articular chondrocytes, which was abolished by hucMSCs‐derived exosomal miR‐100‐5p through its target NOX4. The findings highlight the importance of miR‐100‐5p/NOX4 axis in primary articular chondrocytes injury and provide new insights into therapeutic strategies for articular chondrocytes injury and osteoarthritis.

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“…Further investigations are needed to explain the role of miRNAs in the RAGE–DIAPH1 signalling axis in DM and its neurological complications. The profile of expressed miRNAs and exosomal miRNAs are different in patients with diabetes compared with a control group (Gan et al, 2020; Kim et al, 2020). miRNAs therefore may be also excellent biomarkers for the detection of the onset of diabetic peripheral (length‐dependent) neuropathy (Feng et al, 2016; Hagiwara et al, 2013; Ma et al, 2017; Piperi et al, 2015).…”

Section: The Rage Diaph1 and Another Rage Ligands In Neurological Complications Of Dmmentioning

confidence: 99%

The cross‐talk between RAGE and DIAPH1 in neurological complications of diabetes: A review

Zglejc-Waszak

Mukherjee

Juranek

2021

Eur J of Neuroscience

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Neuropathy, or dysfunction of peripheral nerve, is one of the most common neurological manifestation in patients with diabetes mellitus (DM). DM is typically associated with a hyperglycaemic milieu, which promotes non‐enzymatic glycation of proteins. Proteins with advanced glycation are known to engage a cell‐surface receptor called the receptor for advanced glycation end products (RAGE). Thus, it is reasonable to assume that RAGE and its associated molecule‐mediated cellular signalling may contribute to DM‐induced symmetrical axonal (length‐dependent) neuropathy. Of particular interest is diaphanous related formin 1 (DIAPH1), a cytoskeletal organizing molecule, which interacts with the cytosolic domain of RAGE and whose dysfunction may precipitate axonopathy/neuropathy. Indeed, it has been demonstrated that both RAGE and DIAPH1 are expressed in the motor and sensory fibres of nerve harvested from DM animal models. Although the detailed molecular role of RAGE and DIAPH1 in diabetic neurological complications remains unclear, here we will discuss available evidence of their involvement in peripheral diabetic neuropathy. Specifically, we will discuss how a hyperglycaemic environment is not only likely to elevate advanced glycation end products (ligands of RAGE) and induce a pro‐inflammatory environment but also alter signalling via RAGE and DIAPH1. Further, hyperglycaemia may regulate epigenetic mechanisms that interacts with RAGE signalling. We suggest the cumulative effect of hyperglycaemia on RAGE–DIAPH1‐mediated signalling may be disruptive to axonal cytoskeletal organization and transport and is therefore likely to play a key role in pathogenesis of diabetic symmetrical axonal neuropathy.

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Effect of diabetes on exosomal miRNA profile in patients with obesity

Cited by 32 publications

References 44 publications

Exosomes: Isolation, characterization, and biomedical applications

Exosomes: Isolation, characterization, and biomedical applications

Exosomes from human umbilical cord mesenchymal stem cells inhibit ROS production and cell apoptosis in human articular chondrocytes via the miR‐100‐5p/NOX4 axis

The cross‐talk between RAGE and DIAPH1 in neurological complications of diabetes: A review

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