Effect of Diabetes on the Metabolism of Chenodeoxycholic Acid in Isolated Perfused Rat Liver

Ogura, Yoshio; Ayaki, Yoshikazu

doi:10.1515/bchm3.1987.368.2.813

Cited by 8 publications

(1 citation statement)

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“…A previous paper reported a similarly decreased proportion of β-muricholic acid in the bile of alloxan-induced diabetic mice ( 25 ) . In a study on isolated rat livers, the 6β-hydroxylation of chenodeoxycholic acid to α-muricholic acid and further epimerisation to β-muricholic acid were approximately halved in the liver of streptozotocin-induced diabetic rats ( 26 ) . This effect was counteracted by insulin treatment, suggesting that the lack of insulin signalling leads to decreased synthesis of β-muricholic acid.…”

Section: Discussionmentioning

confidence: 99%

Genetically obese mice do not show increased gut permeability or faecal bile acid hydrophobicity

et al. 2013

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Gut barrier dysfunction may lead to metabolic endotoxaemia and low-grade inflammation. Recent publications have demonstrated gut barrier dysfunction in obesity induced by a diet high in fat, and a pathogenetic role for luminal bile acids has been proposed. We aimed to investigate whether genetically obese mice develop increased gut permeability and alterations in luminal bile acids on a diet with a regular fat content. We used seven obese male ob/ob mice of C57BL/6J background and ten male wild-type (WT) mice of the same strain. Faeces were collected for bile acid analysis. Intestinal permeability was measured in an Ussing chamber upon euthanasia, using 4 kDa fluorescein isothiocyanate dextran, as per mille (‰, 1/1000) of translocated dextran. We analysed the liver expression of lipopolysaccharide-binding protein (LBP), as well as serum LBP (ELISA). Intestinal permeability was not affected by genetic obesity (jejunum: 0·234 (SEM 0·04) ‰ for obese v. 0·225 (SEM 0·03) ‰ for WT, P¼ 0·93; colon: 0·222 (SEM 0·06) ‰ for obese v. 0·184 (SEM 0·03) ‰ for WT, P¼ 0·86), nor was liver LBP expression (relative expression: 0·55 (SEM 0·08) for obese v. 0·55 (SEM 0·13) for WT, P¼ 0·70). Serum LBP was 2·5-fold higher in obese than in WT mice (P¼0·001). Obese mice had increased daily excretion of total bile acids, but their faecal bile acid hydrophobicity was unchanged. In conclusion, genetic obesity did not impair gut barrier function in mice on a regular chow diet, nor was faecal bile acid hydrophobicity affected.

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Section: Discussionmentioning

confidence: 99%