Effect of Diallyl Trisulfide on Human Ovarian Cancer SKOV-3/DDP Cell Apoptosis

Wan, Huifang; Yu, Lushan; Wu, Jin-Lan; Tu, Shuo; Zhu, Wie-Feng; Zhang, Xia-Li; Wan, Fang

doi:10.7314/apjcp.2013.14.12.7197

Cited by 9 publications

(10 citation statements)

References 21 publications

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“…2 ). Previous studies indicate downregulation of Bcl-2 in the presence of DATS in human pancreatic (Ma et al 2014 ), epithelial ovarian (Wan et al 2013 ), leukemia (Choi and Park 2012 ), lung (Li et al 2012 ), breast (Malki et al 2009 ), and prostate (Kim et al 2007 ) cancer cell lines. Bcl-2 protein contains four Bcl-2 homology domains with two cysteine residues at position 158 in the α5 domain and position 229 in the carboxyl-terminal membrane anchor domain (Cys-158 and Cys-229) (Luanpitpong et al 2013 ).…”

Section: Discussionmentioning

confidence: 96%

“…Diallyl trisulfide inhibits the growth of human cancer cells by inducing apoptosis in association with downregulation of Bcl-2 expression (Ma et al 2014 ; Shin et al 2014 ; Zhou et al 2009 ; Li et al 2012 ; Wan et al 2013 ; Malki et al 2009 ; Choi and Park 2012 ; Kim et al 2007 ), induction of caspases and regulation of PI3 K/Akt and JNK pathways (Shin et al 2014 ; Zhou et al 2009 ; Choi and Park 2012 ; Seki et al 2008 ; Borkowska et al 2012 ). DATS-induced apoptosis of human pancreatic cancer cells is correlated with downregulation of Akt and cyclin D1 protein levels, and up-regulation of Bax, Fas, p53 and cyclin B protein levels (Ma et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

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A possible mechanism of inhibition of U87MG and SH-SY5Y cancer cell proliferation by diallyl trisulfide and other aspects of its activity

et al. 2017

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The study was conducted to elucidate the mechanism of antiproliferative and antioxidative action of diallyl trisulfide (DATS), a garlic-derived organosulfur compound. Changes in the l-cysteine desulfuration, and the levels of cystathionine and non-protein thiols in DATS-treated human glioblastoma (U87MG) and neuroblastoma (SH-SY5Y) cells were investigated. The inhibition of proliferation of the investigated cells by DATS was correlated with an increase in the inactivated form of Bcl-2. In U87MG cells, an increased level of sulfane sulfur and an increased activity of 3-mercaptopyruvate sulfurtransferase (MPST) and rhodanese, the enzymes involved in sulfane sulfur generation and transfer, suggest that DATS can function as a donor of sulfane sulfur atom, transferred by sulfurtransferases, to sulfhydryl groups of cysteine residues of Bcl-2 and in this way lower the level of active form of Bcl-2 by S-sulfuration. Diallyl trisulfide antioxidative effects result from an increased level of cystathionine, a precursor of cysteine, and an increased glutathione level. MPST and rhodanese, the level of which is increased in the presence of DATS, can serve as antioxidant proteins.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

A possible mechanism of inhibition of U87MG and SH-SY5Y cancer cell proliferation by diallyl trisulfide and other aspects of its activity

et al. 2017

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“…Recently some of the target genes activated by NF-ĸB (cyclin D1, Bcl-2, Bcl-XL, matrix metallo-proteinases (MMPs) and VEGF) which are very important for cellular transformation, cell proliferation, invasion, migration and metastasis (Sambantham et al, 2013). The other report explains either up-regulation of Bax or decrease in the Bcl-2 protein level after treatment with various cancer drugs resulting in mitochondrial mediated apoptosis (Wan et al, 2013). Together, the present results have demonstrated that fungal taxol could inhibit breast cancer cell line through regulation of various apoptotic signalling proteins.…”

Section: Discussionmentioning

confidence: 99%

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Raj

Shanmugam²,

Manikanadan³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro-as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an IC 50 value of 2.5 μM/ml after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-ĸB, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.

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“…Downstream targets of p53 include members of the B-cell lymphoma 2 (Bcl-2) family of proteins involved in regulating intrinsic apoptosis [ 79 ]. Thus, DATS treatment is known to increase apoptosis by regulating expression of pro-apoptotic Bcl-2-like protein 4 (Bax) [ 38 , 41 , 44 , 45 , 46 , 50 , 61 , 66 , 67 , 71 , 72 , 78 , 80 ], Bcl-2 homologous antagonist (Bak) [ 38 , 50 , 70 , 72 ], BH3 interacting domain death agonist (Bid) [ 38 ], Bcl-2-associated death promoter (Bad) [ 57 ], p53 upregulated modulator of apoptosis (PUMA) [ 50 , 80 ], and phorbol-12-myristate-13-acetate-induced protein 1 (NOXA) [ 50 ] as well as anti-apoptotic Bcl-2 [ 37 , 38 , 41 , 44 , 45 , 50 , 57 , 61 , 66 , 67 , 70 , 71 , 76 , 78 , 80 , 81 , 82 ] and B-cell lymphoma-extra large (Bcl-XL) [ 38 , 45 , 50 , 70 , 78 , 82 ]. Moreover, the pro-apoptotic protein Bcl-2-like protein 11 (Bim), which is a downstream target of JNK, displayed increased phosphorylation following DATS incubation thus providing one connection between DATS treatment, ROS generation, and induction of apoptosis […”

Section: Mechanisms Of Actionmentioning

confidence: 99%

Dietary Bioactive Diallyl Trisulfide in Cancer Prevention and Treatment

Puccinelli

Stan

2017

IJMS

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Bioactive dietary agents have been shown to regulate multiple cancer hallmark pathways. Epidemiologic studies have linked consumption of Allium vegetables, such as garlic and onions, to decreased incidence of cancer. Diallyl trisulfide (DATS), a bioactive compound derived from Allium vegetables, has been investigated as an anti-cancer and chemopreventive agent. Preclinical studies provide ample evidence that DATS regulates multiple cancer hallmark pathways including cell cycle, apoptosis, angiogenesis, invasion, and metastasis. DATS has been shown to arrest cancer cells at multiple stages of the cell cycle with the G2/M arrest being the most widely reported. Additionally, increased pro-apoptotic capacity as a result of regulating intrinsic and extrinsic apoptotic pathway components has been widely reported following DATS treatment. Invasion, migration, and angiogenesis represent emerging targets of DATS and support its anti-cancer properties. This review summarizes DATS mechanisms of action as an anti-cancer and chemopreventive agent. These studies provide rationale for future investigation into its use as a cancer chemopreventive agent.

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Effect of Diallyl Trisulfide on Human Ovarian Cancer SKOV-3/DDP Cell Apoptosis

Cited by 9 publications

References 21 publications

A possible mechanism of inhibition of U87MG and SH-SY5Y cancer cell proliferation by diallyl trisulfide and other aspects of its activity

A possible mechanism of inhibition of U87MG and SH-SY5Y cancer cell proliferation by diallyl trisulfide and other aspects of its activity

Fungal Taxol Extracted from Cladosporium oxysporum Induces Apoptosis in T47D Human Breast Cancer Cell Line

Dietary Bioactive Diallyl Trisulfide in Cancer Prevention and Treatment

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