Effect of diet on carboxylesterase activity of tadpoles ( Rhinella arenarum ) exposed to chlorpyrifos

Attademo, Andrés M.; Sánchez-Hernández, Juan C.; Peltzer, Paola M.; Junges, Celina María

doi:10.1016/j.ecoenv.2016.09.012

Cited by 14 publications

(3 citation statements)

References 50 publications

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“…At the 48 th , 72 nd , and 96 th hours, swimming alterations were only observed at concentrations of 1 and 2 µg/L and were significantly different from the control (p < 0.05) because most of the tadpoles at concentrations of 4 and 8 µg/L had not survived (Table 4). Similar results were found in P. gracilis [36], R. arenarum [48,54], and Lysapsus limellium [54] exposed to chlorpyrifos, but the swimming alterations in F. limnocharis tadpoles even occurred at lower concentrations of chlorpyrifos. This condition is thought to be caused by a decrease in AChE activity because organophos-phate pesticides exposure such as chlorpyrifos triggers the accumulation of acetylcholine in the synaptic cleft which causes continuous contraction of muscle fibers thereby injuring the tissue and changes in motoric function [36,45].…”

Section: Swimming Activitysupporting

confidence: 83%

Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae)

Ramadani

Marhendra

Wiadnya

et al. 2022

J.Trop.Life.Science

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Organophosphate pesticides, especially chlorpyrifos, are one of the most widely used insecticides in agriculture, but their toxicity and potential sensitivity effects on Anura, especially Fejervarya limnocharis are still unknown. The purpose of this investigation is to study F. limnocharis (Anura: Dicroglossidae) tadpole sensitivity to lethal (survivability) and sublethal effects (morphological alterations and swimming activity) of chlorpyrifos in Dursban 200EC commercial formula under acute exposure. An acute toxicity test was carried out on ten tadpoles (Gosner 25) in each repetition. The sample was obtained from artificial reproduction by injecting the Trial Batch 2000 IU hCG by Kings Lab. The acute toxicity testing consisted of three replicates with a chlorpyrifos concentration of 0; 0.4; 0.8; 1; 2; 4; and 8 µg/L. Physico-chemical parameters, mortality, morphological, and swimming alterations of each concentration were observed at 24th, 48th, 72nd, and 96th hours. The LC50 of chlorpyrifos for F. limnocharis tadpoles was 2.86 µg/L. The percentage of survivability F. limnocharis tadpoles decreased after exposure to chlorpyrifos above 1µg/L, while morphological alterations were observed in 2 µg/L and 4 µg/L after 48th hours exposure, and the swimming alterations have occurred at 24th hours in 1; 2; 4 and 8 µg/L. Morphological alterations were observed including asymmetrical body shape, edema, and abnormal tail shape. Based on the LC50 value, commercial chlorpyrifos has high-level toxicity on F. limnocharis tadpoles.Keywords: Acute, Chlorpyrifos, Dursban 200 EC, Fejervarya limnocharis, Tadpoles

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Section: Swimming Activitysupporting

confidence: 83%

Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae)

Ramadani

Marhendra

Wiadnya

et al. 2022

J.Trop.Life.Science

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“…A substantial number of studies suggest an important role for carboxylesterases in the stoichiometric inactivation of organophosphorus compounds and thus the expression of organophosphorus compound toxicity in mammals and amphibians (Caballero de Castro et al ; Attademo et al , ). In the present study, carboxylesterase activity was inhibited in mouse brain, whereas carboxylesterase activity was undetectable in toad brain (Table ).…”

Section: Discussionmentioning

confidence: 99%

“…Several lines of evidence suggest that amphibians may be less sensitive to organophosphorus insecticides because of more effective detoxification (Edery and Schatzberg-Porath 1960;Kerby et al 2010;Crane et al 2016). Carboxylesterases may be important in the relatively lower sensitivity to organophosphorus toxicants exhibited by some amphibians (Robles-Mendoza et al 2011;Attademo et al 2017). Toxicodynamic factors may also play a role.…”

Section: Introductionmentioning

confidence: 99%

Comparative in vitro and in vivo effects of chlorpyrifos oxon in the outbred CD‐1 mouse (Mus musculus) and great plains toad (Anaxyrus cognatus)

Anderson

Liu

McMurry

et al. 2018

Enviro Toxic and Chemistry

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We compared biochemical, functional, and behavioral responses to the organophosphorus anticholinesterase chlorpyrifos oxon (CPO) in mice (Mus musculus, CD-1) and toads (Anaxyrus cognatus, Great Plains toad). Toads were substantially less sensitive to acute lethality of CPO based on the maximum tolerated (nonlethal) dose (toads, 77 mg/kg; mice, 5.9 mg/kg). Sublethal exposures led to classical signs of toxicity (increased involuntary movements, autonomic secretions) in mice but hypoactivity in toads. Motor performance in an inclined plane test was not affected by CPO in mice but was altered at the highest dosage in toads. Acetylcholinesterase (AChE), butyrylcholinesterase, monoacylglycerol lipase, and fatty acid amide hydrolase activities in brain were inhibited in mice but not in toads, and fatty acid amide hydrolase activity in the liver was inhibited in both species. Toad brain AChE was less sensitive to in vitro inhibition by CPO (50% inhibitory concentration [IC50; 20 min, 37 °C], 101 vs 7.8 nM; IC50 [20 min, 26 °C], 149 vs 6.2 nM), and studies of inhibitor kinetics indicated substantially lower anticholinesterase potency of CPO against the toad brain enzyme. Using an in vitro indirect inhibition assay, preincubation of CPO with toad brain homogenate was more effective than an equivalent mouse brain homogenate at reducing CPO potency. These data suggest that the relatively low sensitivity of toads to cholinergic toxicity is based on the low sensitivity of brain AChE, which in turn may be attributable to more effective target-site detoxification. Environ Toxicol Chem 2018;37:1898-1906. © 2018 SETAC.

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Effect of microplastics on the activity of carboxylesterase and phosphatase enzymes in Scinax squalirostris tadpoles

Attademo

Boccioni

Peltzer

et al. 2022

Environ Monit Assess

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Effect of diet on carboxylesterase activity of tadpoles ( Rhinella arenarum ) exposed to chlorpyrifos

Cited by 14 publications

References 50 publications

Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae)

Effect of Acute Toxicity of Commercial Organophosphate Insecticide Based on Chlorpyrifos on Fejervarya limnocharis Tadpoles (Anura: Dicroglossidae)

Comparative in vitro and in vivo effects of chlorpyrifos oxon in the outbred CD‐1 mouse (Mus musculus) and great plains toad (Anaxyrus cognatus)

Effect of microplastics on the activity of carboxylesterase and phosphatase enzymes in Scinax squalirostris tadpoles

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