Vegetation mapping provides important information for understanding ecological condition through calculation of vegetation density. It based on vegetation indices developed through algorithms of a mathematical model within the visible and near-infrared reflectance bands. The index is an estimate of either leaf density per species or vegetation types, respectively. This study aimed to evaluate those indices and find the best algorithm using Sentinel-2 satellite image. Twenty four algorithms of vegetation indices were analyzed for mangrove density mapping, i.e., BR, GNDVI BR, GR, SAVI, MSAVI, NDRE, NDVI, NDVI2, NDWI, NNIP, PSRI, RR, RVI, VIRE, SVI, VIRRE, MTV1, MTVI2, RDVI, VARI, VI green, MSR, and TVI. During pre-processing stage, a digital number of a Sentinel-2 image was converted into radiance and reflectance value. The analysis resulted in three algorithms that provide the highest accuracy, i.e., NDVI (normalized difference vegetation indices) with exponential regression approach, RVI (Ratio Vegetation indices) with the exponential approach and NDVI (normalized difference vegetation indices) with a polynomial approach. The mangrove biomass spatial modeling NDVI with exponential regression approach (RMSE = 89 kg) showed the average of each pixel (10x10m) was 0.97 ton / 100 m2. Total mangrove biomass for above ground and underground vegetation in the study area was 22,365.6 tons. Sentinel-2 satellite image may best use one of those three algorithms, especially applied for mangrove vegetation.

Abstract. Kurniawan A, Hariati AM, Kurniawan A, Wiadnya DGR. 2021. First genetic record and the phylogenetic relationship of Osteochilus spilurus (Cyprinidae: Labeoninae) originating from Bangka and Belitung Islands, Indonesia. Biodiversitas 22: 794-802. Osteochilus spilurus is a freshwater fish from Southeast Asia. Species identification for this fish in Indonesia is still solely limited to morphological characteristics. Therefore it is highly important to validate using short DNA sequences that have been proven to be fast, effective, and accurate. Fish specimens were collected from Lenggang river in Belitung island, and Lebak river in Bangka island, Indonesia. Mitochondrial cytochrome b gene (cyt b, 408 bp) was amplified and sequenced. We collected fish specimens from Lenggang River in Belitung Island and Lebak River in Bangka Island, Indonesia. The results suggested that individuals from both islands are clustered together with a strong bootstrap value (100%) in a monophyletic clade. The specimens deposited at NCBI as the first Indonesian genetic records. They have a sister lineage to individuals from Sabah, Malaysia, although supported by a low bootstrap value. While geographical connection during the Pleistocene period allowed wide distribution of the species, differences in the evolutionary processes of historical biogeography and environmental changes are the predicted cause of the low genetic similarity. These findings can be used as a sequence reference and basic information for conservation of this species.

The mitochondrial genome of a small freshwater fish Rasbora argyrotaenia from Java Island, Indonesia, was completely sequenced. This mitochondrial genome had 16,740 bp in length and consisted of 37 genes in the typical vertebrate mitochondrial gene arrangement. Phylogenetic analysis showed that R. argyrotaenia is more closely related to R. borapetensis than to other Javanese rasboras, R. aprotaenia and R. lateristriata.

Lobster fisheries play an important role in the development of Indonesian fisheries management. The tropical lobster genus Panulirus has high species diversity in the Indonesian Eastern Indian Ocean. The Indonesian government has designated lobster fisheries as one of the types of fisheries requiring special attention in 11 of the Fisheries Management Areas in the Republic of Indonesia (FMARI). Each management area has specific ecological characteristics and may have unique lobster species richness. Studies on the temporal and spatial distribution of lobster species during all life phases are necessary to determine the connectivity of lobster populations. This study aimed to identify the species of lobster larvae of the genus Panulirus in FMARI 573. Samples were collected from several locations, including Pacitan, Trenggalek, Tulungagung, Banyuwangi, and Lombok, in July-August 2018. Data were analysed descriptively through species identification based on morphological traits. Identification of the samples was carried out at the sampling site and in the laboratory. The study found six species of lobster larvae: Panulirus ornatus (local name udang mutiara), P. homarus (udang pasir), P. penicillatus (udang batu), P. versicolor (udang bambu), P. longipes (udang batik) and P. polyphagus (udang Pakistan). These species were unevenly distributed at the study sites. Further research is needed to analyse the distribution and connectivity of lobster populations by analysing species composition during different life phases (larva/puerulus, juvenile, adult) at different spatial and temporal scales.

Shrimp, Penaeus monodon (Fabricius) and Penaeus merguiensis (de Man), culture on East Java is described with special attention to the differences in production characteristics between areas and districts. Traditionally, tambak culture developed along the North coast of East Java where the majority of modern tambaks are still located. The practice never developed extensively on Madura's Island. With the exception of the latter, intensification, startingin 1984‐1987, touched all areas. In 1991, the combined production of P monodon and P. merguiensis reached 35 000 Mt, representing a value of US$ 220 × 106. The following year, a decline in total production was noted, mainly caused by a diminished production of intensive farms. Possible causes for this production drop are explored.