Effect of diet on the replenishment of intramyocellular lipids after exercise

Décombaz, Jacques; Fleith, Mathilde; Hoppeler, Hans; Kreis, Roland; Boesch, Chris

doi:10.1007/s003940070002

Cited by 51 publications

(52 citation statements)

References 13 publications

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“…While the absolute level of IMCL concentrations is muscle-specific, a similar qualitative replenishment could be found (17). In a combined study of 1 H-and 13 C-MRS, Décombaz et al (36) were able to follow the replenishment of the two major energy supplies in skeletal muscle, IMCL and glycogen, under the influence of different diets. A well-controlled type of diet has been investigated in studies with the infusion of lipids and free fatty acids (54,(56)(57)(58).…”

Section: Various Other Influences Upon Imcl Levelsmentioning

confidence: 77%

“…Boesch et al (2) revealed a decrease of IMCL in one subject performing a mountain bike ride over 3 h at about 70% of VO 2max , for the first time using 1 H-MRS to demonstrate that IMCL are metabolized during this form of exercise. In the following years, numerous reports on reduced IMCL levels during exercise were published and reviewed (17,20,(34)(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49). It became obvious that both exercise duration and intensity are important factors in determining the reduction of IMCL (39).…”

Section: Various Other Influences Upon Imcl Levelsmentioning

confidence: 99%

“…This observation led to many studies that investigated the effect of short-term diet on IMCL concentrations (17,20,36,(51)(52)(53)(54)(55). It could be shown that the amount of fat in the diet strongly influences the replenishment of IMCL (17).…”

Section: Various Other Influences Upon Imcl Levelsmentioning

confidence: 99%

“…Several publications have used 13 C-MR spectroscopy to observe muscular glycogen and 1 H-MRS for an evaluation of IMCL (20,36,37,65). This allows the evaluation of fat and carbohydrate metabolism during exercise and diet.…”

Section: Combination Of 1 H-mrs Of Imcl and Other Mrs Techniquesmentioning

confidence: 99%

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Role of proton MR for the study of muscle lipid metabolism

et al. 2006

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1 H-MR spectroscopy (MRS) of intramyocellular lipids (IMCL) became particularly important when it was recognized that IMCL levels are related to insulin sensitivity. While this relation is rather complex and depends on the training status of the subjects, various other influences such as exercise and diet also influence IMCL concentrations. This may open insight into many metabolic interactions; however, it also requires careful planning of studies in order to control all these confounding influences. This review summarizes various historical, methodological, and practical aspects of 1 H-MR spectroscopy (MRS) of muscular lipids. That includes a differentiation of bulk magnetic susceptibility effects and residual dipolar coupling that can both be observed in MRS of skeletal muscle, yet affecting different metabolites in a specific way. Fitting of the intra-(IMCL) and extramyocellular (EMCL) signals with complex line shapes and the transformation into absolute concentrations is discussed. Since the determination of IMCL in muscle groups with oblique fiber orientation or in obese subjects is still difficult, potential improvement with high-resolution spectroscopic imaging or at higher field strength is considered. Fat selective imaging is presented as a possible alternative to MRS and the potential of multinuclear MRS is discussed. 1 H-MRS of muscle lipids allows non-invasive and repeated studies of muscle metabolism that lead to highly relevant findings in clinics and patho-physiology.

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Section: Various Other Influences Upon Imcl Levelsmentioning

confidence: 77%

Section: Various Other Influences Upon Imcl Levelsmentioning

confidence: 99%

Section: Various Other Influences Upon Imcl Levelsmentioning

confidence: 99%

Section: Combination Of 1 H-mrs Of Imcl and Other Mrs Techniquesmentioning

confidence: 99%

See 2 more Smart Citations

Role of proton MR for the study of muscle lipid metabolism

et al. 2006

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“…Using this technique, it has been found that IMCL levels are extremely variable in response to physical exercise 8,11,15,16 and diet. 8,17,18 It has also been shown that different muscle groups contain different levels of IMCL. 7,19,20 The role of IMCL in diabetes is not fully understood, however, an inverse correlation of IMCL levels and insulin sensitivity has been observed by several groups.…”

Section: Susceptibility Effects: Separation Of Imcl and Emclmentioning

confidence: 99%

Dipolar coupling and ordering effects observed in magnetic resonance spectra of skeletal muscle

Boesch

Kreis

2001

NMR in Biomedicine

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Skeletal muscle is a biological structure with a high degree of organization at different spatial levels. This order influences magnetic resonance (MR) in vivo-in particular 1 H-spectra-by a series of effects that have very distinct physical sources and biomedical applications: (a) bulk fat (extramyocellular lipids, EMCL) along fasciae forms macroscopic plates, changing the susceptibility within these structures compared to the spherical droplets that contain intra-myocellular lipids (IMCL); this effect leads to a separation of the signals from EMCL and IMCL; (b) dipolar coupling effects due to anisotropic motional averaging have been shown for 1 H-resonances of creatine, taurine, and lactate; (c) aromatic protons of carnosine show orientation-dependent effects that can be explained by dipolar coupling, chemical shift anisotropy or by relaxation anisotropy; (d) limited rotational freedom and/or compartmentation may explain differences of 1 H-MR-visibility of the creatine/phosphocreatine resonances; (e) lactate 1 H-MR resonances are reported to reveal information on tissue compartmentation; (f) transverse relaxation of water and metabolites show multiple components, indicative of intra-, extracellular and/or macromolecular-bound pools, and in addition dipolar or J-coupling lead to a modulation of the signal decay, hindering straightforward interpretation; (g) diffusion weighted 31 P-MRS has shown restricted diffusion of phosphocreatine; (h) magnetization transfer (MT) indicates that there is a motionally restricted proton pool in spin-exchange with free creatine; reduced availability or restricted motion of creatine is particularly important for an estimation of ADP from 31 P-MR spectra, and in addition MT effects may alter the signal intensity of creatine 1 H-resonances following water-suppression pulses; (i) transcytolemmal water-exchange can be studied in 1 H-MRS by contrast-agents applied to the extracellular space; (k) transport of glucose across the cell membrane has been studied in diabetes patients using a combination of 13 C-and 31 P-MRS; and (l) residual quadrupolar interaction in 23 Na MR spectra from human skeletal muscle suggest that sodium ions are bound to ordered muscular structures.

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Lipid content in the musculature of the lower leg assessed by fat selective MRI: Intra‐ and interindividual differences and correlation with anthropometric and metabolic data

Machann

Bachmann

Brechtel

et al. 2003

Magnetic Resonance Imaging

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Purpose:To assess the muscular lipid content (LC) in different muscle groups of the lower leg by a magnetic resonance imaging technique working with chemical shift selective excitation, and comparison with anthropometric and metabolic data. Materials and Methods:Examinations were performed in 67 volunteers (54 male/13 female, age 29 Ϯ seven years) on a 1.5 T whole body imager, applying a highly selective spectral-spatial technique for fat selective MRI. LC was measured in six calf muscles and correlated with body mass index (BMI), percent body fat (PFAT), and insulin sensitivity (IS) of the subjects. . Insulin-resistant subjects showed slightly but not significantly increased LC compared to insulin-sensitive subjects in BMI-matched subgroups. Conclusion:The fat-selective MRI technique allows a reliable non-invasive measure of muscular lipids -even in muscle groups with inherent low LC -within a relatively short measurement time of about three minutes. The presented data reveal interesting interrelationships between LC and anthropometric and metabolic data, and therefore provide new insight into muscular fat metabolism.

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Effect of diet on the replenishment of intramyocellular lipids after exercise

Abstract: IMCL and glycogen can vary acutely in response to diet after exercise. Studies are needed to determine if such variations occur within the range of ordinary diets and to clarify the functional significance of IMCL in differently active individuals.

Cited by 51 publications

References 13 publications

Role of proton MR for the study of muscle lipid metabolism

Role of proton MR for the study of muscle lipid metabolism

Dipolar coupling and ordering effects observed in magnetic resonance spectra of skeletal muscle

Lipid content in the musculature of the lower leg assessed by fat selective MRI: Intra‐ and interindividual differences and correlation with anthropometric and metabolic data

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