Effect of Diet upon the in vitro Metabolism of Rat Epididymal Adipose Tissue

Giorgio, John Di; Bonanno, Rosemary A.; Hegsted, D. M.

doi:10.1093/jn/78.4.384

Cited by 89 publications

(5 citation statements)

References 18 publications

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“…Hausberger & Milstein (1955) showed a decrease in the conversion in vitro of [14C]glucose into fatty acids in adipose tissue of rats fed on diets containing 13-60% of fat. Di Giorgio, Bonanno & Hegsted (1962), by measuring carbon dioxide evolution from epididymal fat pads in vitro, found lower lipogenesis on diets containing 25% as compared with 5% of fat. They also observed differences in the magnitude of the effect depending on the type of dietary fat given.…”

Section: Resultsmentioning

confidence: 98%

Studies on lipogenesis in vivo. Effect of dietary fat or starvation on conversion of [14C]glucose into fat and turnover of newly synthesized fat

Jansen¹,

Hutchon²,

Zanetti³

1966

Biochemical Journal

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1. Lipogenesis was studied in vivo by giving mice 250mg. meals of [U-(14)C]glucose and measuring the disposition and incorporation of label. About 48% of the (14)C dose was eliminated as (14)CO(2) in the first 2hr. At 60min. after administration, 1.0, 1.9 and 11.9% of the dose was recovered as liver glycogen, liver fatty acid and carcass fatty acid respectively. Of the [(14)C]glucose converted into fat in the epididymal pads about 90% was present as glyceride fatty acid and 10% as glyceride glycerol. 2. Hepatic synthesis of fatty acid was depressed by dietary fat to a much greater extent than was synthesis outside the liver. Both feeding with fat and starvation decreased the proportion of the label taken up by adipose tissue present as fat (triglyceride) and increased the proportion of triglyceride label present as glyceride glycerol. These results are consistent with the hypothesis that the primary action of both these conditions in decreasing fat synthesis is to inhibit synthesis of fatty acids. 3. Turnover of body fat labelled in vivo from [U-(14)C]glucose was estimated from the decline in radioactivity measured over the first 24hr. of the experiment. The half-life of liver and extrahepatic fatty acids (excluding epididymal fat) was 16hr. and 3 days respectively. In contrast, no measurable decrease in radioactivity of the fatty acids of epididymal fat was observed for 7 days after administration of the [U-(14)C]glucose.

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Section: Resultsmentioning

confidence: 98%

Studies on lipogenesis in vivo. Effect of dietary fat or starvation on conversion of [14C]glucose into fat and turnover of newly synthesized fat

Jansen¹,

Hutchon²,

Zanetti³

1966

Biochemical Journal

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“…Fatty acid biosynthesis appears to be regulated :in part by the intake of dietary fat, since incorporation of labelled acetate into fatty acids by animal tissues is depressed by increasing the intake of dietary fat (96)(97)(98). Fatty acid biosynthesis appears to be regulated :in part by the intake of dietary fat, since incorporation of labelled acetate into fatty acids by animal tissues is depressed by increasing the intake of dietary fat (96)(97)(98).…”

Section: Dietary Fat As a Sourcementioning

confidence: 99%

Dietary fat and the fatty acid composition of tissue lipids

Carroll

1965

J Americ Oil Chem Soc

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Some characteristics of the fatty acid conlposition of animal tissue lipids are described and the origins of tissue fatty acids are discussed briefly. The effect of dietary fat on composition of tissue lipids is discussed. Types of dietary fatty acids for which experimental work is described include polyunsaturated fatty acids, short-chain fatty acids, fatty acids with chain length greater than Cts, trans unsaturated fatty acids, fatty acids

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“…Feedback inhibition of fatty acid synthesis is greater during ingestion of unsaturated than saturated fatty acids (Reiser et al, 1963;Hill et al, I960;Di Giorgio et al, 1962;Bottino et al, 1965;Chung et al, 1970).…”

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Oil, Cholesterol, and Soysterols on the Lipid Concentration and Fatty Acid Composition of Egg Yolk, Liver and Serum of Laying Hens

Sim

Bragg

1978

Poultry Science

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Effect of dietary lipid factors (saturated and unsaturated oil, zoo and phytosterols) on the lipid concentration and fatty acid composition of egg yolk, liver and serum of the laying hen were studied. Single Comb White Leghorn laying hens, at 30 weeks of age, were fed two basal diets containing 8.0% hydrogenated coconut oil (HCO) or safflower oil (SFO), with or without supplemental cholesterol (1.0%), soysterols (2.0%) or combination of both. When HCO basal diet was fed, both liver weight and lipid content were significantly (P less than .01) increased as compared to hens fed the SFO diet. Cholesterol feeding increased total lipid content in liver and serum, whereas soysterol feeding reduce or diminish lipid accumulation caused by the cholesterol treatment. Both dietary cholesterol and soysterols alter the fatty acid composition of liver, serum and egg yolk lipids by increasing oleic acid and decreasing palmitic and/or stearic acids. These changes were significantly greater upon feeding cholesterol than soysterols. However, the simultaneous feeding of cholesterol with soysterols exerted the least effect on the fatty acid composition.

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Effect of Diet upon the in vitro Metabolism of Rat Epididymal Adipose Tissue

Cited by 89 publications

References 18 publications

Studies on lipogenesis in vivo. Effect of dietary fat or starvation on conversion of [14C]glucose into fat and turnover of newly synthesized fat

Studies on lipogenesis in vivo. Effect of dietary fat or starvation on conversion of [14C]glucose into fat and turnover of newly synthesized fat

Dietary fat and the fatty acid composition of tissue lipids

Effect of Dietary Oil, Cholesterol, and Soysterols on the Lipid Concentration and Fatty Acid Composition of Egg Yolk, Liver and Serum of Laying Hens

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