Effect of Dietary Astaxanthin on Free Radical Scavenging Capacity and Nitrite Stress Tolerance of Postlarvae Shrimp, Pleoticus muelleri

Abstract

The aim of this study was to investigate the effect of astaxanthin feed supplementation and environmental nitrite stress in postlarvae of Pleoticus muelleri (15 ± 0.004 mg initial weight) under culture conditions. Diets containing three levels of astaxanthin, 0 mg kg(-1) of diet (C0), 100 mg kg(-1) of diet (C(100)), and 300 mg kg(-1) of diet (C(300)), were used. Postlarvae fed with each diet were exposed to different concentrations of nitrite (NO(2)Na) (0-200 mg L(-1)). The 96 h median lethal concentration (LC50) values of nitrite N were 76.3, 89.7, and 157 mg L(-1) for shrimps fed to C0, C(100), and C(300). The scavenging properties were evaluated against the stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical by electron resonance spectroscopy (EPR). For all feed treatments, the extracts exhibited strong DPPH scavenging activity; however, shrimp fed with C(100) and C(300) showed the greatest activity to quench DPPH (62 and 59%, respectively) with respect to C0 (43%). It can be concluded that astaxanthin acts as a protector of nitrite stress in P. muelleri.

Dietary Photoprotective Compounds Ameliorate UV Tolerance in Shrimp (Pleoticus muelleri) through Induction of Antioxidant Activity

“…The potential antioxidant activity of postlarval extracts was determined on the basis of the scavenging activity of the stable 1,1‐diphenyl‐2‐picryhydrazyl (DPPH) free radical, using Electron Paramagnetic Resonance (EPR) with a Bruker ELEXSYS E500T spectrometer. Amplitude signals were transformed into free radical concentrations by comparing the area under the EPR absorption spectrum of the sample to that of a chloroform solution of the concentration standard, DPPH (Díaz et al ). Approximately 25 mg of lyophilized tissue from individuals sampled from each treatment were mixed with 1 mL of chloroform under an argon atmosphere.…”

Carotenoid feed supplementation in Argentine penaeoid shrimp broodstock

“…Aliquots of 50 µL with 50 µL of DPPH solution (1.53×10 -5 M) were analyzed at a constant room temperature of 19.85°C and at fixed time intervals for 60 min. Control or blank preparations containing 50 µL of DPPH in chloroform solvent remained unaltered after an extended period, DPPH proved to be a very stable free radical (Díaz et al, 2014); the intensity of the signal decays with time and represents the radicaltissue reaction. Amplitude signals were transformed in radical concentrations by comparing the area under the EPR absorption spectrum of the sample to a chloroform solution of the concentration standard, DPPH.…”

CAROTENOID-ENRICHED DIET FOR PREMATURATION STAGE OF POND- REARED TIGER SHRIMP, Penaeus monodon: Part II. EFFECT ON GONADAL MATURATION AND BIOCHEMICAL PROFILES OF OOCYTES, SPERMATOPHORES AND HEPATOPANCREAS

“…A high concentration of total carotenoid in oocytes indicated that this micronutrient is importantly required for ovary maturation as many previous studies reported. The important function of astaxanthin in the yolk sac is probably related to its function to improve antioxidant enzyme activities which further increase the tolerance against environmental stress including salinity, pH, oxygen depletion and nitrite stress (Flores et al, 2007;Diaz et al, 2014;Wang et al, 2018) which may happen during the earlier stage of larval development.…”