Effect of dietary cellulose level on specific dynamic action and ammonia excretion of the prawn<i>Macrobrachium rosenbergii</i>(De Man 1879)

González‐Peña, Maria del Carmen; Moreira, Maria da Gloria Blumer Soares

doi:10.1046/j.1365-2109.2003.00887.x

Cited by 13 publications

(6 citation statements)

References 29 publications

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“…In addition, PmChi-2, similar to Pjchi-2, a molt-related chitinase isoform in M. japonicus, is highly expressed in gill especially during intermolt and down-regulated during post-molt. This fluctuating expression pattern during the molt cycle suggests PmChi-2 ′s direct role in molting [47], [48]. Thus, the repertoire of differentially regulated chitinase isoforms in gill and hepatopancreas are expected to be involved in chitin digestion for nutritional purpose, molting or other diversified roles of chitinase in M. rosenbergii.…”

Section: Resultsmentioning

confidence: 99%

In-Depth Tanscriptomic Analysis on Giant Freshwater Prawns

Mohd-Shamsudin

Zhao

et al. 2013

PLoS ONE

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Gene discovery in the Malaysian giant freshwater prawn (Macrobrachium rosenbergii) has been limited to small scale data collection, despite great interest in various research fields related to the commercial significance of this species. Next generation sequencing technologies that have been developed recently and enabled whole transcriptome sequencing (RNA-seq), have allowed generation of large scale functional genomics data sets in a shorter time than was previously possible. Using this technology, transcriptome sequencing of three tissue types: hepatopancreas, gill and muscle, has been undertaken to generate functional genomics data for M. rosenbergii at a massive scale. De novo assembly of 75-bp paired end Ilumina reads has generated 102,230 unigenes. Sequence homology search and in silico prediction have identified known and novel protein coding candidate genes (∼24%), non-coding RNA, and repetitive elements in the transcriptome. Potential markers consisting of simple sequence repeats associated with known protein coding genes have been successfully identified. Using KEGG pathway enrichment, differentially expressed genes in different tissues were systematically represented. The functions of gill and hepatopancreas in the context of neuroactive regulation, metabolism, reproduction, environmental stress and disease responses are described and support relevant experimental studies conducted previously in M. rosenbergii and other crustaceans. This large scale gene discovery represents the most extensive transcriptome data for freshwater prawn. Comparison with model organisms has paved the path to address the possible conserved biological entities shared between vertebrates and crustaceans. The functional genomics resources generated from this study provide the basis for constructing hypotheses for future molecular research in the freshwater shrimp.

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Section: Resultsmentioning

confidence: 99%

In-Depth Tanscriptomic Analysis on Giant Freshwater Prawns

Mohd-Shamsudin

Zhao

et al. 2013

PLoS ONE

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“…Several factors may influence the use of the three different energy substrates (protein, carbohydrates, and lipids) by crustaceans, including diet (Pascual et al, 2004;Hayd et al, 2010;Rocha et al, 2013). In the diets of M. rosenbergii containing different levels of fibre, the O:N ratio varied from 10 (use of carbohydrates) to 49 (use of lipids) (González-Peña and Moreira, 2003). Chen and Nan (1994) observed that different species of marine shrimps fed the same commercial food used different energy substrates: Penaeus japonicus (Bate, 1888) and M. ensis (de Haan, 1844) used proteins, whereas P. monodon (Fabricius, 1798), P. penicillatus (Alcock, 1905), and P. chinensis (Osbeck, 1765) used lipids.…”

Section: Ingestion Rate and Defecationmentioning

confidence: 99%

Are there any physiological differences between the male morphotypes of the freshwater shrimp Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae)?

Augusto

Valenti

2016

Journal of Crustacean Biology

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A comparison was made of the metabolism, nitrogenous excretion, growth, oxidized energy substrate, ingestion rate, and fecal production of the males of three morphotypes (CC, GC1, and GC2) of the palaemonid shrimp Macrobrachium amazonicum (Heller, 1862). The proportion of these morphotypes in the population is fixed, and individuals can change from one type to another (TC to CC, CC to GC1, and GC1 to GC2) to maintain this proportion. The three morphotypes were evaluated for 30 days, during which individuals were fed daily with commercial food. Food debris, feces, and exuviae were collected daily. Oxygen consumption was measured in a closed respirometer, and ammonia excretion was measured by colorimetry. The atomic ratio O:N was used to indicate the predominant energy substrate oxidized. Mass gain (% WW i) was higher in morphotypes CC and GCI (12.7 ± 3.2 and 16.0 ± 3.7%, respectively) than in GC2, in which it was nearly zero (1.9 ± 1.5%). Lost exuviae contained approximately 40% of the energy content of the individuals, and males did not cease feeding as post-molts as reported in some crustaceans. Despite the elevated growth of CC and GC1, the ingestion rates were similar in all morphotypes and corresponded to 3% of the total biomass. It is possible that CC and GC1 channel a higher percentage of ingested energy and nutrients into growth, whereas GC2 channels more energy into other pathways such as reproduction. Whereas the morphotypes CC and GC1 mainly use carbohydrates as their energy substrate, GC2 uses proteins. Given the elevated growth rate of CC and GC1, they appear to preferentially use amino acids in tissue building, whereas GC2 uses these substrates as an energy source. The feces eliminated by the morphotypes were always proportional to the ingestion rate (approximately 2%), suggesting utilization in terms of nutrient absorption was similar for them. Oxygen consumption in specific mass was similar for all three morphotypes (approximately 1.8 μg (mg dw) −1 h −1), and ammonia excretion was approximately 180% higher in GC2 than in the other two morphotypes. These results might reflect the pattern of growth, activity, function in the population, and differences in reproductive behaviour in the morphotypes and could be evidence of a preparation for the subsequent morphotype.

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“…Ammonia is the most common environmental pollutant in aquaculture systems; in fact, it is the main limiting factor in aquaculture practice [ 32 ]. Many studies have shown that ammonia is toxic to aquatic animals, including crustaceans, and it affects survival and the moulting process in decapod species [ 4 , 33 ]. Almost all previous studies reported that ammonia reduces survival in decapod species; however, its effects on moulting appear to be opposite in different species [ 8 , 9 , 34 ].…”

Section: Discussionmentioning

confidence: 99%

Ammonia Stress Disturbs Moult Signaling in Juvenile Swimming Crab Portunus trituberculatus

Wang

Liu

Shang

et al. 2023

Biology

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Ammonia is a significant concern during hatchery culture in brachyuran species, and its accumulation may lead to abortive moulting and large-scale deaths of the early juveniles. To date, the underlying mechanism for ammonia-induced alteration of the moulting process is still unknown. In this study, we aimed to investigate the effects of ammonia on the moulting as well as the potential mechanisms in early juveniles of the swimming crab Portunus trituberculatus, an important aquaculture species in China. We evaluated the survival rate and moulting rate of the juvenile crabs (C2) and analyzed the expression pattern of the genes in key components of molt signaling during a complete moulting cycle under different concentrations of ammonia nitrogen (the control group: <0.1 mg/L; the LA group: 5 mg/L; and the HA group: 20 mg/L). The results showed that: (1) the survival rate in the LA and HA groups was lower than that in the control group at the end of the experiment, and moulting death syndrome (MDS) was only observed in the HA group; (2) the moulting rate was higher in the LA group and lower in the HA group compared to the control group; (3) consistent with the results of the moulting experiment, MIH showed decreased expression, and genes related to ecdysteroid synthesis, ecdysteroid receptors, and responsive effectors exhibited increased expression in the LA group compared to the control group; and (4) although MIH expression was upregulated, increased expression of the genes associated with ecdysteroid synthesis, ecdysteroid receptors and downstream effectors still observed in the HA group. Our results indicated that low levels of ammonia can promote moulting in juvenile swimming crabs by inhibiting the expression of MIH and activating moult signaling, whereas high levels of ammonia inhibit moulting and lead to MDS through impairing moult signaling.

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Effect of dietary cellulose level on specific dynamic action and ammonia excretion of the prawnMacrobrachium rosenbergii(De Man 1879)

Cited by 13 publications

References 29 publications

In-Depth Tanscriptomic Analysis on Giant Freshwater Prawns

In-Depth Tanscriptomic Analysis on Giant Freshwater Prawns

Are there any physiological differences between the male morphotypes of the freshwater shrimp Macrobrachium amazonicum (Heller, 1862) (Caridea: Palaemonidae)?

Ammonia Stress Disturbs Moult Signaling in Juvenile Swimming Crab Portunus trituberculatus

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