Effect of dietary fat on metabolism and DNA adduct formation after acute oral exposure of F-344 rats to fluoranthene☆

Walker, Stormy A.; Addai, Amma B.; Mathis, Malcolm; Ramesh, Aramandla

doi:10.1016/j.jnutbio.2006.04.001

Cited by 23 publications

(16 citation statements)

References 43 publications

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“…Additionally, lipids have been reported to modify the cell membrane structure and function, cell signaling pathways and gene expression and modulate the immune system function (168). Studies conducted by our research group revealed that fluoranthene, a PAH compound damages DNA in several target tissues when administered through saturated fat in F-344 rats (169). Therefore, the question that arises quite naturally is whether the animal models used for PAH carcinogenesis studies can develop colon tumors when they are given unsaturated and saturated fat via oral gavage in the absence of exogenous PAHs.…”

Section: Modifying Factorsmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons and Digestive Tract Cancers: A Perspective

Diggs

Huderson

Harris

et al. 2011

Journal of Environmental Science and Health, Part C

241

107

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Cancers of the colon are most common in the Western world. In majority of these cases, there is no familial history and sporadic gene damage seems to play an important role in the development of tumors in the colon. Studies have shown that environmental factors, especially diet, play an important role in susceptibility to GI tract cancers. Consequently, environmental chemicals that contaminate food or diet during its preparation becomes important in the development of GI cancers. Polycyclic aromatic hydrocarbons (PAHs) are one such family of ubiquitous environmental toxicants. These pollutants enter the human body through consumption of contaminated food, drinking water, inhalation of cigarette smoke, automobile exhausts, and contaminated air from occupational settings. Among these pathways, dietary intake of PAHs constitutes a major source of exposure in humans. Although many reviews and books on PAHs and their ability to cause toxicity and breast or lung cancer have been published, aspects on contribution of diet, smoking and other factors towards development of digestive tract cancers and strategies to assess risk from exposure to PAHs have received much less attention. This review, therefore, focuses on dietary intake of PAHs in humans, animal models, and cell cultures used for GI cancer studies along with epidemiological findings. Bioavailability and biotransformation processes, which influence the disposition of PAHs in body and the underlying causative mechanisms of GI cancers, are also discussed. The existing data gaps and scope for future studies is also emphasized. This information is expected to stimulate research on mechanisms of sporadic GI cancers caused by exposure to environmental carcinogens.

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Section: Modifying Factorsmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons and Digestive Tract Cancers: A Perspective

Diggs

Huderson

Harris

et al. 2011

Journal of Environmental Science and Health, Part C

241

107

View full text Add to dashboard Cite

show abstract

“…Our current findings suggest that olive oil is more than likely assisting with an increase in bioavailability of B(a)P due to its monounsaturated nature. Our earlier studies have shown that polyunsaturated fats (a class to which olive oil belongs) favor increased bioavailability of PAHs [66,67]. Laher et al [68] first demonstrated that olive oil facilitates the lymphatic transport of B(a)P through rapid absorption in the enterocytes.…”

Section: 0 Discussionmentioning

confidence: 99%

Olive oil prevents benzo(a)pyrene [B(a)P]-induced colon carcinogenesis through altered B(a)P metabolism and decreased oxidative damage in ApcMin mouse model

Banks

Amoah

Niaz

et al. 2016

The Journal of Nutritional Biochemistry

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Colon cancer ranks third in cancer related mortalities in the United States. Many studies have investigated factors that contribute to colon cancer in which dietary and environmental factors have been shown to play an integral role in the etiology of this disease. Specifically, human dietary intake of environmental carcinogens such as polycyclic aromatic hydrocarbons (PAHs) has generated interest in looking at how it exerts its effects in gastrointestinal carcinogenesis. Therefore, the objective of this study was to investigate the preventative effects of olive oil on benzo(a)pyrene [B(a)P]-induced colon carcinogenesis in adult ApcMin mice. Mice were assigned to a control (n =8) or treatment group (n =8) consisting of 25, 50 and 100 μg B(a)P/kg body weight (bw) dissolved in tricaprylin [B(a)P-only group] or olive oil daily via oral gavage for sixty days. Our studies showed that ApcMin mice exposed to B(a)P developed a significantly higher number (p< 0.05) of larger dysplastic adenomas compared to those exposed to B(a)P + olive oil. Treatment of mice with B(a)P and olive oil significantly altered (p< 0.05) the expression of drug metabolizing enzymes in both the colon and liver tissues. However, only GST activity was significantly higher (p< 0.05) in the liver of mice treated with 50 and 100 μg B(a)P/kg bw + olive oil. Lastly, olive oil promoted rapid detoxification of B(a)P by decreasing its organic metabolite concentrations and also decreasing the extent of DNA damage to colon and liver tissues (p< 0.05). These results suggest that olive oil has a protective effect against B(a)P-induced colon tumors.

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“…In addition to lipids, chloroformmethanol can successfully retrieve PAHs from other binding sites such as proteins [18]. It needs to be mentioned that chloroform-methanol mixture has also been found to be effective in extracting BaP and fluoranthene (another PAH compound) from colon and liver tissues of mice and rats respectively in our laboratory [19][20][21].…”

Section: Methodsmentioning

confidence: 99%

Comparative Evaluation of Different Cell Lysis and Extraction Methods for Studying Benzo(a)pyrene Metabolism in HT-29 Colon Cancer Cell Cultures

Myers

Rekhadevi

Ramesh

2011

Cell Physiol Biochem

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Lysis and extraction of cells are essential sample processing steps for investigations pertaining to metabolism of xenobiotics in cell culture studies. Of particular importance to these procedures are maintaining high lysis efficiency and analyte integrity as they influence the qualitative and quantitative distribution of drug and toxicant metabolites in the intra- and extracellular milieus. In this study we have compared the efficiency of different procedures viz. homogenization, sonication, bead beating, and molecular grinding resin treatment for disruption of HT-29 colon cells exposed to benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH) compound and a suspected colon carcinogen. Also, we have evaluated the efficiency of various procedures for extracting BaP parent compound/metabolites from colon cells and culture media prior to High Performance Liquid Chromatography (HPLC) analyses. The extraction procedures include solid phase extraction, solid-supported liquid- liquid extraction, liquid-liquid extraction, and homogeneous liquid- liquid extraction. Our findings showed that bead-beating in combination with detergent treatment of cell pellet coupled with liquid-liquid extraction yielded greater concentrations of BaP metabolites compared to the other methods employed. Our method optimization strategy revealed that disruption of HT-29 colon cells by a combination of mechanical and chemical lysis followed by liquid-liquid extraction is efficient and robust enough for analyzing BaP metabolites from cell culture studies.

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Effect of dietary fat on metabolism and DNA adduct formation after acute oral exposure of F-344 rats to fluoranthene☆

Cited by 23 publications

References 43 publications

Polycyclic Aromatic Hydrocarbons and Digestive Tract Cancers: A Perspective

Polycyclic Aromatic Hydrocarbons and Digestive Tract Cancers: A Perspective

Olive oil prevents benzo(a)pyrene [B(a)P]-induced colon carcinogenesis through altered B(a)P metabolism and decreased oxidative damage in ApcMin mouse model

Comparative Evaluation of Different Cell Lysis and Extraction Methods for Studying Benzo(a)pyrene Metabolism in HT-29 Colon Cancer Cell Cultures

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