Effect of Dietary Fiber on Pancreatic Enzyme Activity In Vitro

Isaksson, G; Lundquist, Ingmar; Ihse, Ingemar

doi:10.1016/s0016-5085(82)80256-4

Cited by 166 publications

(32 citation statements)

References 11 publications

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“…If the in uitro observations reported here occur in oiuo then the effects on the colonic flora and environment would be complex. Viscosities in the upper intestine after fasting are low, around 30 mPa.s for duodenal juice (Isaksson et a/. 1982).…”

Section: Discussionmentioning

confidence: 97%

Response of populations of human faecal bacteria to viscosity in vitro

Wyatt¹,

Archer²

1988

J Appl Microbiol

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Section: Discussionmentioning

confidence: 97%

Response of populations of human faecal bacteria to viscosity in vitro

Wyatt¹,

Archer²

1988

J Appl Microbiol

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“…Although some studies have already reported the possible interference of dietary fibres in pancreatic lipase-catalysed triacylglycerol lipolysis [15][16][17][18], the mechanisms involved have not been investigated in detail and no data are available on gastric lipase which begins hydrolysis of dietary fat in the stomach [1][2][3][4][5][6]. Two pieces of information have been obtained in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The effects of dietary fibres on digestion, gut physiology and lipid metabolism have been extensively studied but the various mechanisms involved are still under investigation [13,14]. Several studies have reported that some sources of dietary fibre can lower the activity of pancreatic lipase in itro [15][16][17][18], fat lipolysis in the stomach and small intestine [19] and lipid absorption in i o [20]. Moreover, viscous fibres have been reported to increase the viscosity of the digestive contents [21][22][23], regulate digestive organ motility [24], delay stomach emptying, delay and\or reduce the absorption of nutrients and alter postprandial response [19][20][21][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Emulsification and lipolysis of triacylglycerols are altered by viscous soluble dietary fibres in acidic gastric medium in vitro

Pasquier

Armand

Castelain

et al. 1996

Biochemical Journal

107

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This in vitro study was designed to test the hypothesis that soluble dietary fibres can alter the process of intragastric lipid emulsification and possibly subsequent triacylglycerol lipolysis. Three guar gums, two pectins and gum arabic were dissolved in acidic gastric medium in the concentration range 0.3-2.0% (w/v). Viscosities of fibre solutions were measured and apparent viscosities varied over a wide range (0.7-77 mPa/s). Emulsification of a lipid mixture (triolein/phosphatidylcholine/cholesterol) was performed under mild conditions in the presence of increasing concentrations of soluble fibres. The amount of emulsified lipid was not affected whereas the size of the emulsified droplets was increased by raising the concentration of viscous fibres only. The droplet size (r=0.75, P=0.006) and overall droplet surface area (r=-0.69, P=0.009) were strongly correlated with the medium viscosity in the range 0-20 mPa/s. The addition of solutions of viscous fibres to a preformed standard emulsion did not change the initial velocity of human gastric lipase reaction. Conversely, when emulsions prepared in the presence of fibres (i.e. with different droplet sizes) were incubated with excess gastric enzyme for 2 h, the high-viscosity guar gum significantly reduced the extent of triacylglycerol lipolysis, as compared with control and low- or medium-viscosity fibres. In conclusion, the data obtained show that reducing emulsification of dietary lipids in the mildly acid medium found in the stomach is a mechanism by which soluble viscous fibres can alter lipid assimilation.

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“…Pectin, a fiber found in fruits, has been shown to increase chymotrypsin, whereas wheat and oat brans have been shown to decrease amylase activity [19]. Pectin has been shown to reduce the activity of amylase in duodenal fluid in vitro [23]. Furthermore, non-soluble dietary fibers may have direct molecular interaction and adsorb enzymes which also alters pancreatic enzyme activity [19,24].…”

Section: Discussionmentioning

confidence: 99%

Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach

et al. 2020

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Although the gut microbiome has been associated with dietary patterns linked to health, microbial metabolism is not well characterized. This ancillary study was a proof of principle analysis for a novel application of metaproteomics to study microbial protein expression in a controlled dietary intervention. We measured the response of the microbiome to diet in a randomized crossover dietary intervention of a whole-grain, low glycemic load diet (WG) and a refined-grain, high glycemic load diet (RG). Total proteins in stools from 9 participants at the end of each diet period (n = 18) were analyzed by LC MS/MS and proteins were identified using the Human Microbiome Project (HMP) human gut microbiome database and UniProt human protein databases. T-tests, controlling for false discovery rate (FDR) <10%, were used to compare the Gene Ontology (GO) biological processes and bacterial enzymes between the two interventions. Using shotgun proteomics, more than 53,000 unique peptides were identified including microbial (89%) and human peptides (11%). Forty-eight bacterial enzymes were statistically different between the diets, including those implicated in SCFA production and degradation of fatty acids. Enzymes associated with degradation of human mucin were significantly enriched in the RG diet. These results illustrate that the metaproteomic approach is a valuable tool to study the microbial metabolism of diets that may influence host health.

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Effect of Dietary Fiber on Pancreatic Enzyme Activity In Vitro

Cited by 166 publications

References 11 publications

Response of populations of human faecal bacteria to viscosity in vitro

Response of populations of human faecal bacteria to viscosity in vitro

Emulsification and lipolysis of triacylglycerols are altered by viscous soluble dietary fibres in acidic gastric medium in vitro

Gut Microbial Protein Expression in Response to Dietary Patterns in a Controlled Feeding Study: A Metaproteomic Approach

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