Response of populations of human faecal bacteria to viscosity in vitro

Wyatt, G. M.; Archer, David B.

doi:10.1111/j.1365-2672.1988.tb04430.x

Cited by 2 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results showed that gut bacteria growth displayed different behavior regarding viscosity depending on bacteria species. This fact is in accordance with preliminary studies where it was reported that viscosity influences in viscotaxis (bacteria motility) (Petrino & Doetsch, 1978) and in microbial population composition (Wyatt & Archer, 1988).…”

Section: Resultssupporting

confidence: 93%

Influence of viscosity on the growth of human gut microbiota

et al. 2018

View full text Add to dashboard Cite

Numerous studies support the beneficial effects of dietary fibre. It is well known that fibre increases viscosity at intestinal level. Therefore, the effects of fibre on gut microbiota could be due not only by its intestinal bacteria fermentation but also to the increase in viscosity by itself. The aim of this study was to evaluate the effect of viscosity on the growth of gut microbiota at physiological conditions. For this purpose, four compartments from a gastrointestinal simulator (simgi®) were filled with Gut Nutrient Medium (GNM) plus different agar concentrations (0, 0.30, 0.45 and 0.60 %), inoculated with faecal microbiota, and incubated 48 h under anaerobic conditions. Samples were collected at three time points (0, 24 h and 48 h) for representative intestinal bacterial enumeration and rheological characterization. Incubation of GNM gels with faecal microbiota changed the medium viscosity over time, even with constant conditions (temperature and pH). In such way that, in absence of agar (low viscosity), viscosity slightly increased over time; however, in viscous mediums, viscosity decreased over time. In relation to the growth of gut microbiota, results showed that viscosity favoured the growth of total anaerobes and Clostridium spp.; in contrast, total number of aerobes and members of Enterococcus correlated negatively with viscosity increment. In conclusion, changes in intestinal viscosity seem to selectively modify microbiota composition. This is a pioneer work to understand the effect of food viscosity in the gastrointestinal system, showing that viscosity is an important factor itself to condition the growth of different bacteria's groups.

show abstract

Section: Resultssupporting

confidence: 93%

Influence of viscosity on the growth of human gut microbiota

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Nevertheless, not only can the type of medium have an effect on bacterial cultivability but also the viscosity impacts on the spectrum of micro-organisms isolated (Wyatt & Archer, 1988).…”

Section: Culture Mediamentioning

confidence: 99%

Optimizing culture methods for diagnosis of prosthetic joint infections: a summary of modifications and improvements reported since 1995

Larsen

Lange

et al. 2012

Journal of Medical Microbiology

View full text Add to dashboard Cite

Improving diagnosis of prosthetic joint infections (PJIs) has become an increasing challenge due to a steadily rising number of patients with prosthetic implants. Based on a systematic literature search we have ascertained the evidence base for improvement of culture diagnosis. We searched PubMed/MEDLINE using the medical subject heading (MeSH) 'prosthesis-related infections' 1995 through 2010 without further restrictions. An analogous search was conducted for ISI Web of Knowledge. A total of 1409 reports were screened for original results, obtained by methods described in sufficient detail to make replication possible. We gave priority to methods for sample preparation, culture media, culture methods and incubation time. Clinical sensitivity and specificity were calculated where possible. We found evidence to support superiority of cultures obtained from the diluent after sonication of prosthetic implants in comparison with culturing tissue biopsies. Sonication parameters and accessory steps have been studied extensively, and thresholds for significant growth have been defined. Conversely, methods for processing of soft tissue biopsies have been studied to a limited extent. Culture of synovial fluid in blood culture vials has been shown to be more sensitive (90-92 %) than intraoperative swab cultures (68-76 %) and tissue cultures (77-82 %). Formal evaluation of agar media for culturing PJI specimens seemed to be lacking. The polymicrobial nature of PJIs supports the routine use of an assortment of media suitable for recovery of fastidious, slow-growing, anaerobic and sublethally damaged bacteria. A number of studies supported an incubation period for up to 14 days. Although we identified evidence-based improvements of culture methods, there is a need for more studies especially with regard to tissue biopsies. Culturing remains an important means to identify and characterize pathogenic micro-organisms and supplements the increasing number of cultureindependent assays.

show abstract

Response of populations of human faecal bacteria to viscosity in vitro

Cited by 2 publications

References 19 publications

Influence of viscosity on the growth of human gut microbiota

Influence of viscosity on the growth of human gut microbiota

Optimizing culture methods for diagnosis of prosthetic joint infections: a summary of modifications and improvements reported since 1995

Contact Info

Product

Resources

About