Effect of dietary fish oil on enhanced inflammation and disturbed lipophagy in white adipose tissue caused by a high fat diet

Saitoh, Kanae; Yoshimura, Tomohiro; Sun, Luchuanyang; Yang, Min; Wang, Yao; Taniyama, Shigeto; Hara, Kenji; Murayama, Fumihito; Nikawa, Takeshi; Tachibana, Kunihide; Hirasaka, Katsuya

doi:10.1007/s12562-019-01374-4

Cited by 3 publications

(3 citation statements)

References 38 publications

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“…Previous studies demonstrated that fish oil supplements given to obese human subjects decreased expression of inflammasome-associated IL-18 and IL-1β and circulating IL-18 levels, and other adipose inflammatory genes ( 16 ). Saitoh et al ( 17 ) have reported that supplementation with fish oil reduced macrophage infiltration and cytokine levels in mice white adipose tissue contributing to inhibit the insulin resistance caused by a high-fat diet through an anti-inflammatory effect. However, other studies have shown that chronic supplementation of fish oil might alter lipid signaling in obese adipose tissue suggesting dysregulation of adipose tissue expansion and inflammatory signaling ( 18 ).…”

Section: Introductionmentioning

confidence: 99%

Fish Oil Improves Pathway-Oriented Profiling of Lipid Mediators for Maintaining Metabolic Homeostasis in Adipose Tissue of Prediabetic Rats

et al. 2021

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Adipose tissue is now recognized as an active organ with an important homeostatic function in glucose and lipid metabolism and the development of insulin resistance. The present research investigates the role of lipid mediators and lipid profiling for controlling inflammation and the metabolic normal function of white adipose tissue from rats suffering from diet-induced prediabetes. Additionally, the contribution to the adipose lipidome induced by the consumption of marine ω-3 PUFAs as potential regulators of inflammation is addressed. For that, the effects on the inflammatory response triggered by high-fat high-sucrose (HFHS) diets were studied in male Sprague-Dawley rats. Using SPE-LC-MS/MS-based metabolo-lipidomics, a range of eicosanoids, docosanoids and specialized pro-resolving mediators (SPMs) were measured in white adipose tissue. The inflammatory response occurring in prediabetic adipose tissue was associated with the decomposition of ARA epoxides to ARA-dihydroxides, the reduction of oxo-derivatives and the formation of prostaglandins (PGs). In an attempt to control the inflammatory response initiated, LOX and non-enzymatic oxidation shifted toward the production of the less pro-inflammatory EPA and DHA metabolites rather than the high pro-inflammatory ARA hydroxides. Additionally, the change in LOX activity induced the production of intermediate hydroxides precursors of SPMs as protectins (PDs), resolvins (Rvs) and maresins (MaRs). This compensatory mechanism to achieve the restoration of tissue homeostasis was significantly strengthened through supplementation with fish oils. Increasing proportions of ω-3 PUFAs in adipose tissue significantly stimulated the formation of DHA-epoxides by cytochrome P450, the production of non-enzymatic EPA-metabolites and prompted the activity of 12LOX. Finally, protectin PDX was significantly reduced in the adipose tissue of prediabetic rats and highly enhanced through ω-3 PUFAs supplementation. Taken together, these actively coordinated modifications constitute key mechanisms to restore adipose tissue homeostasis with an important role of lipid mediators. This compensatory mechanism is reinforced through the supplementation of the diet with fish oils with high and balanced contents of EPA and DHA. The study highlights new insides on the targets for effective treatment of incipient diet-induced diabetes and the mechanism underlying the potential anti-inflammatory action of marine lipids.

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Section: Introductionmentioning

confidence: 99%

Fish Oil Improves Pathway-Oriented Profiling of Lipid Mediators for Maintaining Metabolic Homeostasis in Adipose Tissue of Prediabetic Rats

et al. 2021

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“…In addition, some active substances were reported to regulate HFD-induced lipophagy dysfunction. Saitoh et al revealed that dietary fish oil could alleviate HFD-enhanced inflammation and disturbed lipophagy in the white adipose tissue . Moreover, creatine ameliorated HFD-induced obesity by regulating lipolysis and lipophagy in the brown adipose tissue and liver …”

Section: Introductionmentioning

confidence: 99%

“…Saitoh et al revealed that dietary fish oil could alleviate HFDenhanced inflammation and disturbed lipophagy in the white adipose tissue. 12 Moreover, creatine ameliorated HFD-induced obesity by regulating lipolysis and lipophagy in the brown adipose tissue and liver. 13 Enhancing lipophagy by adding dietary nutrient components can alleviate lipid accumulation without affecting the quality of life.…”

Section: Introductionmentioning

confidence: 99%

Sea Cucumber Plasmalogen Regulates the Lipid Profile in High-Fat Diet Mouse Liver via Lipophagy

Wang,

Liu

et al. 2024

J. Agric. Food Chem.

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The sea cucumber plasmalogen PlsEtn has been shown to be associated with various chronic diseases related to lipid metabolism. However, the mechanism is unclear. Therefore, the present study used the sea cucumber plasmanylcholine PakCho as a structural contrast to PlsEtn and assessed its effect in 8 week high-fat diet (HFD)-fed mice. The lipidomic approach based on highresolution mass spectrometry combined with molecular biology techniques was used to evaluate the mechanism of PlsEtn. The results showed that both PlsEtn and PakCho significantly inhibited an increase in mouse body weight and liver total triglyceride and total cholesterol levels caused by HFD. In addition, oil red O staining demonstrated that lipid droplets stored in the liver were degraded. Meanwhile, untargeted lipidomic experiments revealed that total lipids (increased by 42.8 mmol/mg prot; p < 0.05), triglycerides (increased by 38.9 mmol/mg prot; p < 0.01), sphingolipids (increased by 1.5 mmol/mg prot; p < 0.0001), and phospholipids (increased by 2.5 mmol/mg prot; p < 0.05) were all significantly elevated under HFD. PlsEtn resolved lipid metabolism disorders by alleviating the abnormal expression of lipid subclasses. In addition, five lipid molecular species, PE (18:1/ 20:4), PE (18:1/20:3), PE (18:1/18:3), TG (16:0/16:0/17:0), and TG (15:0/16:0/18:1), were identified as the biomarkers of HFD-induced lipid metabolism disorders. Finally, lipophagy-associated protein expression analysis showed that HFD abnormally activated lipophagy via ULK1 phosphorylation and PlsEtn alleviated lipophagy disorder through lysosomal function promotion. In addition, PlsEtn performed better than PakCho. Taken together, the current study results unraveled the mechanism of PlsEtn in alleviating lipid metabolism disorder and offered a new theoretical foundation for the high-value development of sea cucumber.

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Dietary ω-3 fatty acids and their influence on inflammation via Toll-like receptor pathways

Jalili

Hekmatdoost

2021

Nutrition

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Effect of dietary fish oil on enhanced inflammation and disturbed lipophagy in white adipose tissue caused by a high fat diet

Cited by 3 publications

References 38 publications

Fish Oil Improves Pathway-Oriented Profiling of Lipid Mediators for Maintaining Metabolic Homeostasis in Adipose Tissue of Prediabetic Rats

Fish Oil Improves Pathway-Oriented Profiling of Lipid Mediators for Maintaining Metabolic Homeostasis in Adipose Tissue of Prediabetic Rats

Sea Cucumber Plasmalogen Regulates the Lipid Profile in High-Fat Diet Mouse Liver via Lipophagy

Dietary ω-3 fatty acids and their influence on inflammation via Toll-like receptor pathways

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