Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium

Zaima, Nobuhiro; Kinoshita, Saori; Hieda, Nao; Kugo, Hirona; Narisawa, Kaori; Yamamoto, Ayami; Yanagimoto, Kenichi; Moriyama, Tatsuya

doi:10.1016/j.bbrep.2016.06.014

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…More fatty acids (FFA) were produced from an increased concentration of acetyl-CoA, which reacting with 3-glycerol, produced more phosphoglycerides and phophatidylcholine (Figure 2D), but switched off the glycerol shuttle with a decrease of NADH production (Figure 2D). This is in agreement with Zaima et al [26], who found that testosterone levels were upregulated in mice that received fish oil with high eicosapentaenoic acid content. In the testicular interstitial, eicosapentaenoic acid containing phosphatidylcholine was distributed, characteristically demonstrating the involvement of eicosapentaenoic acid in testosterone metabolism.…”

Section: Discussionsupporting

confidence: 92%

Plasma Metabolomics Profile of “Insulin Sensitive” Male Hypogonadism after Testosterone Replacement Therapy

Zolla

Ceci

2022

IJMS

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Male hypogonadism is a disorder characterized by low levels of testosterone, but patients can either show normal insulin (insulin-sensitive (IS)) or over time they can become insulin-resistant (IR). Since the two groups showed different altered metabolisms, testosterone replacement therapy (TRT) could achieve different results. In this paper, we analyzed plasma from 20 IS patients with low testosterone (<8 nmol/L) and HOMAi < 2.5. The samples, pre- and post-treatment with testosterone for 60 days, were analyzed by UHPLC and mass spectrometry. Glycolysis was significantly upregulated, suggesting an improved glucose utilization. Conversely, the pentose phosphate pathway was reduced, while the Krebs cycle was not used. Branched amino acids and carnosine metabolism were positively influenced, while β-oxidation of fatty acids (FFA) was not activated. Cholesterol, HDL, and lipid metabolism did not show any improvements at 60 days but did so later in the experimental period. Finally, both malate and glycerol shuttle were reduced. As a result, both NADH and ATP were significantly lower. Interestingly, a significant production of lactate was observed, which induced the activation of the Cori cycle between the liver and muscles, which became the main source of energy for these patients without involving alanine. Thus, the treatment must be integrated with chemicals which are not restored in order to reactivate energy production.

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Section: Discussionsupporting

confidence: 92%

Plasma Metabolomics Profile of “Insulin Sensitive” Male Hypogonadism after Testosterone Replacement Therapy

Zolla

Ceci

2022

IJMS

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“…53 Regarding the steroidogenic functions of the testis, ω-3 treatment induced significant elevation of testicular testosterone levels, AR expression, and concomitant elevation of 17β-HSD gene expression pointing to the beneficial effects on steroidogenesis and Leydig cell functions. Zaima et al, 54 reported the beneficial effects of fish oil supplementation on testicular testosterone in intact mice; they indicated their crucial role in testosterone metabolism in Leydig cells. A remarkable decrease in MDA and elevation of SOD activity indicates the role of ω-3 in restoring the oxidantantioxidant balance in the testis of T2DM rats through modulation of oxidative stress leading to decreased inflammatory status.…”

Section: Gene's Expressions Levelsmentioning

confidence: 99%

Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats

Hasan

El‐Shal

Mackawy

et al. 2019

J of Cellular Biochemistry

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Background Chronic hyperglycemia is linked to either subfertility or infertility among diabetic males. Pioglitazone is one of the thiazolidinediones (TZDs) drugs that are selective peroxisome proliferator‐activated receptor (PPAR‐γ agonists used for treating type 2 diabetes mellitus (T2DM). Aim This study aims to explore the possible effect of low Pioglitazone dose and omega (ω‐3) on rat male reproductive function. Furthermore, we evaluated the add‐on effect of combined use of low Pioglitazone dose of and ω‐3 on reproductive functions in adult male T2DM rats. Methods Fifty adult male rats were included and subdivided into control and four test subgroups. T2DM was induced in test groups and subdivided into non‐treated T2DM, ω‐3 treated, 0.6 mg/kg Pioglitazone treated, and combined treated group (orally by gavage). Following 16 weeks, final body weight, testicular weight, fasting plasma glucose, and serum testosterone levels were measured. Semen analysis, testicular testosterone, malondialdehyde (MDA) concentrations, superoxide dismutase (SOD) activity, immunohistochemistry staining for apoptosis marker B‐cell lymphoma protein 2 (Bcl‐2), proliferation marker as proliferating cell nuclear antigen (PCNA), estrogen receptor α (ERα), androgen receptor (AR) were determined. Caspase‐3, nuclear factor‐kappa B (NF‐kB), glucose transporter 3 (GLUT3), 17β‐hydroxysteroid dehydrogenases (17β‐HSD) PPARγ, and PPARα genes expression were analyzed by real‐time polymerase chain reaction (RT‐PCR). Results Our findings revealed that treatment with low dose of Pioglitazone or ω‐3 significantly lowered fasting plasma glucose and MDA levels, ameliorated diabetes effects on histological damage, improved antioxidant activity (SOD), significantly improved anti‐apoptosis BCL‐2 and proliferation (PCNA), remarkably elevated ERα, AR, 17β‐HSD PPARγ, and PPARα expression with significant reduction in caspase‐3, NF‐kB genes expression and improved semen quality as well. Combined use of low dose of and ω‐3 has better effects on all measured parameters. Conclusion Small Pioglitazone dose and ω‐3 possess beneficial effects on spermatogenic and steroidogenic functions in adult diabetic rat; while combined use of both has an add‐on effect.

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“…They also have a protective role against oxidative damage of P 450 systems in Leydig cells [ 42 ]. The EPA of FO was found to have a master role in testosterone metabolism, combined into the testicular-interstitium cells plasma membrane in a distinctive way, also It was found to be arranged in Leydig cells with unknown manner [ 43 ]. Moreover, our results revealed that NCF co-treatment with CP significantly alleviated the reduction in serum testosterone concentration compared to the CP-untreated group for about 3.4-fold.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical and Biological Evaluation of a Newly Designed Nutraceutical Self-Nanoemulsifying Self-Nanosuspension for Protection and Treatment of Cisplatin Induced Testicular Toxicity in Male Rats

et al. 2021

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The incorporation of cisplatin (CP) as a cytotoxic antineoplastic agent in most chemotherapeutic protocols is a challenge due to its toxic effect on testicular tissues. Natural compounds present a promising trend in research, so a new nutraceutical formulation (NCF) was designed to diminish CP spermatotoxicity. A combination of three nutraceutical materials, 250 mg Spirulina platensis powder (SP), 25 mg Tribulus terrestris L. extract (TT), and 100 mg fish oil (FO) were formulated in self-nanoemulsifying self-nanosuspension (SNESNS). SP was loaded into the optimized self-nanoemulsifying system (30% FO, 50% span 80/cremophor EL and 20% isopropanol) and mixed with TT aqueous solution to form SNESNS. For the SP, phytochemical profiling revealed the presence of valuable amounts of fatty acids (FAs), amino acids, flavonoids, polyphenols, vitamins, and minerals. Transmission electron microscopy (TEM) and particle size analysis confirmed the formation of nanoemulsion-based nanosuspension upon dilution. Method validation of the phytochemical constituents in NCF has been developed. Furthermore, NCF was biologically evaluated on male Wistar rats and revealed the improvement of spermatozoa, histopathological features, and biochemical markers over the CP and each ingredient group. Our findings suggest the potential of NCF with SNESNS as a delivery system against CP-induced testicular toxicity in male rats.

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Effect of dietary fish oil on mouse testosterone level and the distribution of eicosapentaenoic acid-containing phosphatidylcholine in testicular interstitium

Cited by 9 publications

References 11 publications

Plasma Metabolomics Profile of “Insulin Sensitive” Male Hypogonadism after Testosterone Replacement Therapy

Plasma Metabolomics Profile of “Insulin Sensitive” Male Hypogonadism after Testosterone Replacement Therapy

Ameliorative effect of combined low dose of Pioglitazone and omega‐3 on spermatogenesis and steroidogenesis in diabetic rats

Phytochemical and Biological Evaluation of a Newly Designed Nutraceutical Self-Nanoemulsifying Self-Nanosuspension for Protection and Treatment of Cisplatin Induced Testicular Toxicity in Male Rats

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