Effect of dietary supplementation of inulin and W. cibaria on haemato-immunological parameters of hybrid surubim (Pseudoplatystoma sp)

Abstract

The dietary supplementation of prebiotics, probiotics and symbiotic in hybrid surubins (a Pseudoplatystoma corruscans and P. fasciatum cross) was evaluated for the effects on their autochthonous intestinal microbiota and on haematological and immunological parameters. A total of 160 fish were divided into four treatment groups with four replicates each. The treatment groups were fed with the following diets for 15 days: control diet without supplementation; 0.5% inulin (prebiotic) supplementation; Weissella cibaria (CPQBA 001-10 DRM 02) (7.87 ± 0.2 log CFU g )1 ) supplementation; or 0.5% inulin and W. cibaria supplementation (symbiotic group). The midgut intestines of the fish with the symbiotic diet supplementation had higher concentrations of lactic acid bacteria (7.07 ± 1.11 log CFU g )1 ) and low levels of Vibrio spp (1.90 ± 0.60 log CFU g )1 ) and Pseudomonas spp (2.23 ± 1.48 log CFU g )1 ). In addition, increased erythrocytes and reduced circulating neutrophils were observed in this group. No differences in blood glucose, serum protein or lysozyme levels were detected between treatment groups. However, a higher concentration of total immunoglobulin was observed in fish fed with the probiotic and symbiotic diets. The addition of 0.5% inulin (prebiotic) thus W. cibaria (probiotic) to the diet of Pseudoplatystoma hybrid surubins reduce the number of pathogenic bacteria and stimulate the beneficial intestinal microbiota and may possibly alter their immune defence system.

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“…The increased number of lactic acid bacteria observed in the gut of juvenile fish fed with a diet containing inulin, is in agreement with results of Akrami, Hajimoradloo, Matinfar and Abedian Kinari (2009) investigated the effects of inulin on the intestinal microflora of Beluga (H. huso) juvenile and Mourino, Nascimento, Vieria, Jatoba, Silva, Jesus, Seiffert and Martins (2012) studied the effects of inulin and Weissella cibaria on hybrid surubium (Pseudoplatystoma sp). In gut, the increase in shortchain fatty acids and lactic acid from the fermentation of inulin leads to a decrease in pH.…”

Selection of autochtone probiotic for Astyanax bimaculatus

“…For diseases of bacterial origin, these compounds are widely used, but their indiscriminate use in various activities, including aquaculture, has resulted in the emergence of resistant bacterial strains (Bloch et al, 2013). As an alternative, the use of probiotics has emerged (Balcázar et al, 2008;Newaj-fyzul et al, 2014;Raja e Jithendran, 2015). Probiotics may be defined as live microorganisms that colonize the digestive tract of aquaculture animals in order to improve their health (Gatesoupe, 1999), and in the aquaculture industry, they are mainly used as a prophylactic measure (Ringø et al, 2014).…”

Growth and intestinal morphology of juvenile pacu Piaractus mesopotamicus (Holmberg 1887) fed dietary prebiotics (mannanoligosaccharides - MOS)

“…Hybrid surubim Pseudoplatystoma sp. fed 0.5 and 1.0% dietary inulin showed no effect on pathogenic bacteria population numbers when compared to fish fed control diet (Mouriño et al 2012). In addition, Reza et al (2009) The present study was performed in controlled laboratory hearing conditions.…”