Effect of different benzyladenine time pulses on the endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in micropropagated explants of Actinidia deliciosa

Moncaleán, Paloma; Rodrı́guez, Ana; Fernández, Belén

doi:10.1016/s0981-9428(02)00023-2

Cited by 11 publications

(8 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Growth regulators (especially cytokinin) are one of the most important factors affecting shoot proliferation (Moncalean et al 2003;Norton and Norton 1985). In this study, growing shoots on MS medium supplemented with TDZ and high concentration (2.0 mg/L) of BA and Kin resulted in compact dark callus formation at the base of the shoots.…”

Section: Discussionmentioning

confidence: 72%

Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.

et al. 2016

View full text Add to dashboard Cite

Plant tissue culture can readily be applied to the field of medicinal plants and secondary metabolites production. In this study, in vitro propagation protocol of Rumex cyprius Murb. was established. The highest shoot multiplication rate was observed from explants grown on MS medium supplemented with 1.5 mg/L BA (7.8 shoots per explant). For rooting, the maximum root induction rate was observed on MS medium supplemented with 1.5 mg/L IBA or 1.0 mg/L NAA. The longest roots were observed for plants grown on MS medium supplemented with 1.5 mg/L IBA. For callus induction, leaves from in vitro grown plants were cultured in various levels of 2,4-D or different combinations of BA and NAA. The highest fresh weight (5.86 g) of calli was observed at 1.5 and 0.5 mg/L BA and NAA, respectively. The second part of this work investigated the effects of abiotic factors (NaCl and Mannitol) and biotic factors (Yeast extract and Chitosan) on secondary metabolites accumulation of in vitro grown R. cyprius. Phenolic compounds were extracted, and the individual phenolic compounds and antioxidant activity of the extracts were determined of in vitro grown R. cyprius. The total phenol content and antioxidant activity increased significantly by the pervious factors. Reversed high-pressure liquid chromatography (RP-HPLC) was used to measure the effect of these factors on the contents of individual phenolic compound. New phenolic compounds (Gallic acid and Chlorogenic acid) not found in R. cyprius plants grown on control media were produced in R. cyprius plants grown on media supplemented with elicitors. Results indicate that using elicitors in plant tissue culture could be used to produce/enhance secondary metabolites accumulation in R. cyprius.

show abstract

Section: Discussionmentioning

confidence: 72%

Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Root CKs that showed significant differences between treatments also showed significant relationships to root growth and root water variables, and with pH at both the aerial and root levels. DHZ type CKs, being the most stable of the CKs in oxidative environments (Moncaleán et al, 2003), seem to be principally responsible for this behavior. The lack of between-treatment differences in CK content at the shoot level seems to indicate that, during the analyzed period, these compounds do not act as a rootto-shoot drought signals.…”

Section: Discussionmentioning

confidence: 95%

Rapid responses of C14 clone of Eucalyptus globulus to root drought stress: Time-course of hormonal and physiological signaling

Granda¹,

Cuesta²,

Álvarez³

et al. 2011

Journal of Plant Physiology

Self Cite

View full text Add to dashboard Cite

“…The relative abundance of different CKs can vary greatly between plant species, tissues and developmental stages, and depends on the environmental conditions (Frébort et al ). Some studies have examined the effect of varying the culture atmosphere on endogenous CK levels in angiosperms (Feito et al , Moncaleán et al ) and gymnosperms (Moncaleán et al ). Recently, Zhang et al () studied the effect of BA application, to reinvigorate P. radiata buds, in some endogenous CK metabolites.…”

Section: Introductionmentioning

confidence: 99%

Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees

Montalbán

Novák

Rolčı́k

et al. 2012

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

In Pinus radiata D. Don, the transition from the juvenile to the mature phase is characterized by a reduction in the tree's organogenic potential, which is usually reverted in breeding programs by reinvigoration procedures to enable vegetative propagation. In this work, we have determined the best culture conditions for in vitro reinvigoration of radiata pine buds, tested different cytokinin (CK) types [N⁶-benzyladenine (BA), meta-topolin (mT) and trans-zeatin] and concentrations (25 and 50 µM), and studied the effect of culture conditions on endogenous CK and indole-3-acetic acid (IAA) levels at different stages of the organogenic process. To this end, the levels of 43 CKs and IAA were determined in P. radiata buds before and during the reinvigoration process. When BA or mT was applied to the induction medium, we did not observe any significant increase or decrease in endogenous isoprenoid CK content. We also report for the first time the presence of O-glucosides in non-treated P. radiata explants from the field and remark the importance of O-glucosides as storage forms.

show abstract

Effect of different benzyladenine time pulses on the endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in micropropagated explants of Actinidia deliciosa

Cited by 11 publications

References 33 publications

Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.

Enhancement of phenolic compounds production in in vitro grown Rumex cyprius Murb.

Rapid responses of C14 clone of Eucalyptus globulus to root drought stress: Time-course of hormonal and physiological signaling

Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees

Contact Info

Product

Resources

About