Effect of Different Chromosome Numbers of Tobacco Pollen on Plant Formation by Anther Culture and Fertilization

Genetic control of chromosomal chimerism found in a regenerate from tobacco callus.

“…Limiting citations to studies on the genus Nicotiana, a number of investigations have been made on cytological chimeras in cultured tissues (Fox 1963;Cooper et al 1964;Niizeki 1974;Novak and Vyskot and others), and in regenerated plants (Sacristan and Melchers 1969;Collins et al 1972;Niizeki and Kita 1975;Ogura 1975aOgura , 1976 and others) as well as in intact plants or hybrids (Kostoff 1930;Yang 1965;Gerstel and Burns 1966 and others). Ogura (1975) reported that a plant regenerated by a combination of a morphactin, chlorflurenol (CFM) and kinetin (KIN) exhibited variation in its chromosome number, even in the second selfed generation.…”

The Cytological Chimeras in Original Regenerates From Tobacco Tissue Cultures and in Their Offsprings

“…The variation of somatic chromosome number in tobacco regenerates has also been reported by Sacristan and Melchers (1969), Ogura (1975a) and Niizeki and Kita (1975). Tabata et al (1968) and Zagorska et al (1974) have reported their results on cytological analyses of PMC's at the first meiotic metaphase in the original regenerates.…”

“…Limiting to the studies on the genus Nicotiana, a great number of investigations have been made on cytological chimeras in cultured tissues (Fox 1963;Cooper et al 1964;Shimada and Tabata 1967;Murashige and Nakano 1967) and in the regenerated plants (Sacristan and Melchers 1969;Ogura 1975;Niizeki and Kita 1975), as well as in intact plants or hybrids (Moav 1957;Yang 1964Yang , 1965Gerstel and Burns 1966). However, only a few literatures dealing with the progeny plants of regenerates derived from tissue cultures have been elaborated.…”

Androgenesis in Vitro in Tobacco

“…Pre-treatments and growing of the donor plants It was found that the pre-treatment of the excised flower buds or anthers increase the frequency of androgenesis (94, 130). Numerous methods have been tested including water stress, ABA, anaerobic treatment (38, 59, 60, 61), application of antiauxins or auxin-synthesis inhibitors (34) but most effective appeared to be chilling for 2-3 days at 4°C (36,53,90,111,133,134,145). Some authors claim that this treatment switches the pollen development to embryogenesis (127).…”