The Cytological Chimeras in Original Regenerates From Tobacco Tissue Cultures and in Their Offsprings

Ogura, Hisakazu

doi:10.1266/jjg.51.161

Cited by 47 publications

(7 citation statements)

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“…In terms of the chromosomal constitution of the regenerated plants from callus cultures, the results of cytological observations are classified into the following two types; the eudiploid or polyploid constitution of original regenerates (Mitra et al 1960;Blakely and Steward 1964;Venketeswaran and Spiess 1964;Singh et al 1972;Novak and Vyskot 1975 and others) and the aneuploid constitution of the regenerates (Tabata et al 1968;Niizeki and Grant 1971;Melchers and Labib 1974;Smith et al 1976 and others) as well as the somatic chromosomal chimerism of the regenerates (mainly in the Nicotiana and Saccharum species) (Sacristan and Melchers 1969;Heinz et al 1969;Heinz and Mee 1971;Collins et al 1972;Ogura 1975aOgura , 1976Liu and Chen 1976 and others) . The different results may be due to nature of the species used, culture conditions and other unknown causes.…”

Section: Discussionmentioning

confidence: 99%

“…Limiting citations to studies on the genus Nicotiana, a number of investigations have been made on cytological chimeras in cultured tissues (Fox 1963;Cooper et al 1964;Niizeki 1974;Novak and Vyskot and others), and in regenerated plants (Sacristan and Melchers 1969;Collins et al 1972;Niizeki and Kita 1975;Ogura 1975aOgura , 1976 and others) as well as in intact plants or hybrids (Kostoff 1930;Yang 1965;Gerstel and Burns 1966 and others). Ogura (1975) reported that a plant regenerated by a combination of a morphactin, chlorflurenol (CFM) and kinetin (KIN) exhibited variation in its chromosome number, even in the second selfed generation.…”

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Genetic control of chromosomal chimerism found in a regenerate from tobacco callus.

Ogura

1978

The Japanese journal of genetics

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Genetic control of chromosomal chimerism found in a regenerate from tobacco callus.

Ogura

1978

The Japanese journal of genetics

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“…D'autre part, la totipotence de la cellule végétale a pu être démontrée de manière irréfuta-ble grâce à la culture de cellules isolées de Tabac (Vasil et Hildebrandt, 1965 ;Lutz, 1966 Lutz (1966), Butenko et coll. (1967), Lutz (1967), Sacristan (1967), Lutz (1969), Sacristan et Melchers (1969), Lutz (1970), Sacristan et Lutz (1970), Mousseau (1970), Lutz (1971), Syono et Furuya (1972), Ogura (1976), Lutz (1977), Barbier et Dulieu (1980), de Boucaud (1982, 1983.…”

Section: A) Un Matériel De Choix :Le Tabacunclassified

L' Expression de la variabilité morphogénétique après régénération dans les cultures de tissus et de cellules

Lutz

1985

Bulletin de la Société Botanique de France. Actualités Botaniqu

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Résumé.-Les cultures de tissus et de cellules subissent au cours de leur développement in vitro des variations plus ou moins importantes. La variabilité morphogénétique chez les organes et les plantes issus c!e ces cultures est fonction de l'importance de ce phénomène et, parfois même, de l'état initial des cellules. Elle touche généralement la morphologie, le port de la plante, la forme des feuilles, des inflorescences, des fleurs et sc répercute sur la fertilité, mais on rencontre aussi d'autres anomalies. Elle prend très souvent de l'ampleur avec l'âge des souches tissulaires et peut conduire à la production de véritables monstres. Au sein des exemples choisis pour l'illustrer, le Tabac a été pris comme exemple type. Des cas particuliers de variations sont également décrits. L'article sc termine par quelques réflexions sur cette variabilité et les espoirs qu'elle suscite.Summary.-More or less important variations occur during the development of tissues and cells cultures. The morphogenetic variability in organs and plants derived from these cultures is depending upon the importance of this phenomenon and even sometimes upon the initial state of the cells. Variations observed generally include changes in plant morphology and habit, leaves, inflorescences and flowers shape, and plant fertility. Variability increascs very often with the age of the cultures and may lead to the production of real monsters. Among the various plants used to illustra te this variability, tobacco has been choscn as sample plant. Particular cases of variations are also described. Sorne comments about variability and their resulting perspectives for plant impro· vement are presented.

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“…In tobacco, Nicotiana tabacum L., somaclonal variant plants with normal, aneuploid, polyploid, and mixoploid chromosome numbers have been reported [16]. Gill et al [7] recently reported variant potato, Solanum tuberosum L., protoclones with normal chromosome numbers, but structural aberrations including a translocation, inversion, small deficiencies, and a possible duplication.…”

Section: Introductionmentioning

confidence: 99%

Morphologic, cytogenetic, and enzymatic variation in tissue culture regenerated plants of apomictic old-world bluestem grasses (Bothriochloa sp.)

Taliaferro

Dabo

Mitchell

et al. 1989

Plant Cell Tiss Organ Cult

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Somaclonal variant plants may be of use in broadening the germplasm base of plant species and providing useful stocks for cytogenetic investigations. This study was conducted to compare morphologic, cytogenetic and enzymatic characteristics of 21 R~ (initial regenerate) bluestem, Bothrioehloa sp., plants, visibly identified in a field-grown population of 522 plants as probable variants, with their respective R 0 (explant donor) progenitor. An R, seedling population was grown to ascertain the transmission of the variant R~ phenotypes. All R~ plants differed from their respective Ro progenitors in one or more morphological characters. Foliage colour was the most pronounced difference in most cases. Four of the plants, three of which were dwarfed, produced no inflorescences. The R, plants tended to be shorter than R 0 progenitors and had corresponding decreases in lengths on inflorescences and lowest racemes. All R, plants of accessions 8911C and 8793 had an increase in chromosome number from 2n = 4x = 40 to 2n = 5x = 50. Three dwarfed Rh plants, derived from accession 8873B, were aneuploids, two having 2n = 48 chromosomes and the third being a probable mixoploid with 55-58 chromosomes. Other plants of accession 8873B had the R0 chromosome number. Fertility, as estimated by pollen stainability and seed set, generally was reduced in R, plants relative to the R 0. This reduction was not drastic, however, with all flowering plants having 45% or higher seed set. Apomixis apparently maintained fertility in all R~ plants, including those with a pentaploid chromosome number. All R t plants differed from their respective R 0 plants in peroxidase and esterase banding patterns. All R t plants of accessions 891 IC, and 8793, respectively, had identical peroxidase and esterase bands. For both enzyme systems two banding patterns were present in R~ plants of accession 8873B, with 12 of 13 plants exhibiting common patterns. Examination of R 2 progeny plants confirmed the genetic transmission of the variant phenotypes and, by virtue of uniformity, indicated apomictic reproduction in the R~ plants. The results demonstrate the production of potentially useful genetic and cytogenetic variant plants via tissue culture in these apomictic species.

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The Cytological Chimeras in Original Regenerates From Tobacco Tissue Cultures and in Their Offsprings

Cited by 47 publications

References 23 publications

Genetic control of chromosomal chimerism found in a regenerate from tobacco callus.

Genetic control of chromosomal chimerism found in a regenerate from tobacco callus.

L' Expression de la variabilité morphogénétique après régénération dans les cultures de tissus et de cellules

Morphologic, cytogenetic, and enzymatic variation in tissue culture regenerated plants of apomictic old-world bluestem grasses (Bothriochloa sp.)

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