Effect of different concentrations of resveratrol on the quality and in vitro fertilizing ability of ram semen stored at 5 °C for up to 168 h

Al-Mutary, Mohsen Ghaleb; Al‐Ghadi, Muath Q.; Ammari, Aiman A.; Al-Himadi, Ahmed R.; Al-Jolimeed, Abdulaziz H.; Arafah, Mohammed Waleed; Amran, Ramzi A.; Aleissa, Mohammed S.; Swelum, Ayman A.

doi:10.1016/j.theriogenology.2020.05.001

Cited by 27 publications

(17 citation statements)

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“…Substantial improvements in preserved (liquid or freezing storage) semen quality traits and sperm fertilization [ 130 , 131 , 133 , 134 ], oocyte development and competence, and embryo development [ 15 , 16 , 139 , 140 ] have been reported. The other evidence that could be observed from in vitro studies is that supplementation of polyphenolic compounds during gametes or embryo in vitro manipulation might continue to in vivo animals.…”

Section: Polyphenols and In Vitro Assisted Reproduction Techniquesmentioning

confidence: 99%

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

2020

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Reproduction is a complex process that is substantially affected by environmental cues, specifically feed/diet and its components. Farm animals as herbivorous animals are exposed to a large amount of polyphenols present in their natural feeding system, in alternative feed resources (shrubs, trees, and agro-industrial byproducts), and in polyphenol-enriched additives. Such exposure has increased because of the well-known antioxidant properties of polyphenols. However, to date, the argumentation around the impacts of polyphenols on reproductive events is debatable. Accordingly, the intensive inclusion of polyphenols in the diets of breeding animals and in media for assisted reproductive techniques needs further investigation, avoiding any source of reproductive waste and achieving maximum benefits. This review illustrates recent findings connecting dietary polyphenols consumption from different sources (conventional and unconventional feeds) with the reproductive performance of farm animals, underpinned by the findings of in vitro studies in this field. This update will help in formulating proper diets, optimizing the introduction of new plant species, and feed additives for improving reproductive function, avoiding possible reproductive wastes and maximizing possible benefits.

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Section: Polyphenols and In Vitro Assisted Reproduction Techniquesmentioning

confidence: 99%

Polyphenols in Farm Animals: Source of Reproductive Gain or Waste?

2020

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“…Inc., Smithfield, UT, USA). Cumulus-oocyte complexes (COCs) with uniformly granulated cytoplasm and more than three layers of compact cumulus cells were chosen and evaluated (Al mutary et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

“…COCs with more than 3 layers of cumulus cells and uniform cytoplasm (n ¼ 2641) were matured for 24 h in TCM-199 Earle's salts supplemented with 10% fetal bovine serum (FBS), 0.3 mM sodium pyruvate, 1 mg/ml of estradiol-17b, 0.023 IU/ml of luteinizing hormone (LH), 0.02 IU/ml of follicular stimulating hormone (FSH) and 25 mg/ml of gentamycin sulfate at 38.5 °C and exposed to 5% carbon dioxide (CO2) in air at high humidity. The oocytes (10-15 per droplet) were cultured in 60 mm Petri dishes containing 10 drops, each 50 ml in size and were covered with embryotested mineral oil (Al mutary et al, 2020).…”

Section: Methodsmentioning

confidence: 99%

Morphometric Assessment of the Bovine Ovary for in vitro Matured Oocyte Quality to Determine Developmental Competence

Ammari

Amran

Ghadi

et al. 2022

IJAR

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Background: Embryo production in vitro requires three steps: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture (IVC). The first step in the in vitro maturation of oocytes is germinal vesicle breakdown (GVBD), followed by completion of the 1st meiotic division and formation of the 1st polar body. These parameters are critical during ovary and oocyte selection. This study aimed to evaluate bovine ovary and oocyte collection for IVM. Methods: Ovaries collected from cows in a 0.9% NaCl saline solution at the central slaughterhouse were divided into two normal and abnormal ovaries according to their morphological appearance. The morphometric dimensions of bovine ovaries, such as weight and deamination (length, width and volume), were recorded. The oocyte was extruded and its deamination occurred before and after culture in maturation medium. Result: The mean ovary weight, volume, length and width were not significantly different between the two ovary types. Additionally, the dominant and subordinate follicle diameters in both ovary types showed no significant differences. Furthermore, the oocyte number per ovary of the normal and abnormal oocytes showed no significant differences. The mean cumulus oocyte complex before maturation showed no significant difference (52.73±7.23 mm for the normal ovary vs. 43.015±5.41 mm for the abnormal ovary). However, after maturation, a highly significant difference was found (P less than 0.001) between the normal ovaries before and after maturation (178.10±15.36 µm) and abnormal ovaries (10.45±7.99 µm). Additionally, data analysis of oocytes with or without 1st polar bodies revealed a highly significant difference (P less than 0.001) between oocytes of the normal ovary with (32.15±4.19) and without (10.95±1.59) 1st polar bodies and oocytes of the abnormal ovary with (7.0±078) and without 1st polar bodies (3.3±0.32). Thus, the critical point at which the normal ovary produces better in vitro matured follicles with good oocyte quality and produces the 1st polar body determines developmental competence. Therefore, the best selection of normal ovaries will enhance in vitro maturation for subsequent experiments, such as in vitro fertilization or cloning and in vitro embryo development.

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“…Several studies have revealed the antioxidative properties of resveratrol (3,5,4-trihydroxystilbene, polyphenol found in some fruits like grapes) and its ability to reduce mitochondrial ROS production, scavenge superoxide radicals, decrease apoptosis, and inhibit lipid peroxidation as well as regulate the expression of antioxidant cofactors and enzymes [105] [106]. In a recent study, resveratrol supplementation (200, 400 mM) to Tryladyl ® extender have shown to protect and maintain ram sperm head morphology and enhance some motion characteristics during chilled storage at 5˚C up to 7 days likely via its antioxidant potential as indicated by MDA, SOD, and GSH indexes [107]. Moreover, the IVF test revealed that the highest cleavage and blastocyst rates were achieved with spermatozoa treated with 400 mM resveratrol at 5˚C for 72 h.…”

Section: Plant Extractsmentioning

confidence: 99%