Effect of different glucogenic to lipogenic nutrient ratios on rumen fermentation and bacterial community
            <i>in vitro</i>

Hua, Dengke; Zhao, Yiguang; Nan, Xuemei; Xue, Fuguang; Wang, Y.; Jiang, Linshu; Xiong, Benhai

doi:10.1111/jam.14873

Cited by 8 publications

(5 citation statements)

References 78 publications

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“…Ruminobacter , an amylolyic bacterium able to degrade proteins, was also found at a higher level in the rumen with dietary supplementation with A. nodosum or L. digitata [ 67 ]. A previous study showed that DMD and gas production were positively correlated with Ruminobacter [ 68 ], which was confirmed by the rumen fermentation data of this study. The relative abundance of Fibrobacter , another main cellulose-degrading bacterium in the rumen [ 69 ], was also significantly increased by the addition of PT.…”

Section: Discussionsupporting

confidence: 90%

Effects of Phlorotannins from Sargassum on In Vitro Rumen Fermentation, Microbiota and Fatty Acid Profile

Huang,

Chen,

Wang

et al. 2023

Animals

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The fatty acid profiles of ruminant-derived products are closely associated with human health. Ruminal microbiota play a vital role in modulating rumen biohydrogenation (BH). The aim of this study was to assess the influence of dietary supplementation with phlorotannins (PTs) extracted from Sargassum on rumen fermentation, fatty acid composition and bacterial communities by an in vitro culture study. The inclusion of PTs in the diet increased dry matter digestibility and gas production, and reduced ammonia-N concentration and pH. PT extract inhibited rumen BH, increasing the content of trans-9 C18:1, cis-9 C18:1, trans-9 and trans-12 C18:2 and reducing C18:0 concentration. 16S rRNA sequencing revealed that PTs caused an obvious change in rumen bacterial communities. The presence of Prevotella decreased while carbohydrate-utilizing bacteria such as Prevotellaceae_UCG-001, Ruminococcus, Selenomonas, Ruminobacter and Fibrobacter increased. Correlation analysis between rumen FA composition and the bacterial microbiome revealed that Prevotellaceae_UCG-001, Anaerovorax, Ruminococcus, Ruminobacter, Fibrobacter, Lachnospiraceae_AC2044_group and Clostridia_UCG-014 might have been involved in the BH process. In conclusion, the results suggest that the inclusion of PTs in the diet improved rumen fermentation and FA composition through modulating the rumen bacterial community.

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Section: Discussionsupporting

confidence: 90%

Effects of Phlorotannins from Sargassum on In Vitro Rumen Fermentation, Microbiota and Fatty Acid Profile

Huang,

Chen,

Wang

et al. 2023

Animals

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“…NH 3 -N concentrations are associated with the rate of nitrogen use by rumen microorganisms. Some functional rumen bacteria, such as Prevotella and Quinella , can improve the digestibility of dietary protein and metabolism of amino acids, resulting in the production of high concentrations of NH 3 -N ( 65 , 66 ). This result is consistent with the greater abundance of Prevotella and the higher concentrations of NH 3 -N in the Tibetan sheep of the OHF group.…”

Section: Discussionmentioning

confidence: 99%

Shift of Feeding Strategies from Grazing to Different Forage Feeds Reshapes the Rumen Microbiota To Improve the Ability of Tibetan Sheep (Ovis aries) To Adapt to the Cold Season

et al. 2023

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During the cold season, like other high-altitude mammals, Tibetan sheep have to adapt their physiological and nutritional strategies, as well as the structure and function of their rumen microbial community, to the seasonal variation of lower food availability and quality. This study focused on the changes and adaptability in the rumen microbiota of Tibetan sheep when they adapted from grazing to a high-efficiency feeding strategy during the cold season by analyzing the rumen microbiota of Tibetan sheep raised under the different management systems, and it shows the linkages among the rumen core and pan-bacteriomes, nutrient utilization, and rumen short-chain fatty acids.

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“…The principal coordinates analysis (PCoA) was analyzed with the method of unweighted UniFrac distance to compare the interrelationships of bacterial communities between diets using the R software (3.4.4). The community richness and diversity were analyzed by the alpha diversity indexes including the OTU, Chao 1, ACE, Shannon, and Simpson (Hua et al, 2021).…”

Section: Illumina Miseq Sequencing and Analysismentioning

confidence: 99%

Glucogenic and lipogenic diets affect in vitro ruminal microbiota and metabolites differently

Hua

Hendriks²,

Zhao³

et al. 2022

Front. Microbiol.

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This study was conducted to evaluate the effects of two glucogenic diets (C: ground corn and corn silage; S: steam-flaked corn and corn silage) and a lipogenic diet (L: sugar beet pulp and alfalfa silage) on the ruminal bacterial and archaeal structures, the metabolomic products, and gas production after 48 h in vitro fermentation with rumen fluid of dairy cows. Compared to the C and S diets, the L dietary treatment leaded to a lower dry matter digestibility (DMD), lower propionate production and ammonia-nitrogen concentration. The two glucogenic diets performed worse in controlling methane and lactic acid production compared to the L diet. The S diet produced the greatest cumulative gas volume at any time points during incubation compared to the C and L diet. The metabolomics analysis revealed that the lipid digestion especially the fatty acid metabolism was improved, but the amino acid digestion was weakened in the L treatment than in other treatments. Differences in rumen fermentation characteristics were associated with (or resulting from) changes in the relative abundance of bacterial and archaeal genera. The rumen fluid fermented with L diet had a significantly higher number of cellulolytic bacteria, including the genera of Ruminococcus, Butyrivibrio, Eubacterium, Lachnospira, unclassified Lachnospiraceae, and unclassified Ruminococcaceae. The relative abundances of amylolytic bacteria genera including Selenomonas_1, Ruminobacter, and Succinivibrionaceae_UCG-002 were higher in samples for diets C and S. The results indicated that the two glucogenic diets leaded to a higher relative abundance of bacteria which functions in succinate pathway resulting in a higher propionate production. The steam-flaked corn diet had a higher gas production and lower level of metabolites in fatty acids and amino acids. Most highly abundant bacteria were observed to be not sensitive to dietary alterations of starch and fiber, except for several amylolytic bacteria and cellulolytic bacteria. These finding offered new insights on the digesting preference of ruminal bacteria, which can assist to improve the rumen functioning.

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Effect of different glucogenic to lipogenic nutrient ratios on rumen fermentation and bacterial community in vitro

Cited by 8 publications

References 78 publications

Effects of Phlorotannins from Sargassum on In Vitro Rumen Fermentation, Microbiota and Fatty Acid Profile

Effects of Phlorotannins from Sargassum on In Vitro Rumen Fermentation, Microbiota and Fatty Acid Profile

Shift of Feeding Strategies from Grazing to Different Forage Feeds Reshapes the Rumen Microbiota To Improve the Ability of Tibetan Sheep (Ovis aries) To Adapt to the Cold Season

Glucogenic and lipogenic diets affect in vitro ruminal microbiota and metabolites differently

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